Search PubMed⌕ Search

Biomedical subjects

Ia Kh Turakulov

Publications and source records attributed to Ia Kh Turakulov.

At least 19 recordsLinked to original sources
PubMed1993

[The effect of thyroid hormones and 5-azacytidine on the gene transcription of malic enzyme and 6-phosphogluconate dehydrogenase].

The effect of thyroid hormones and the methylation inhibitor 5-azacytidine on the level of expression of thyroid hormone-responsive genes (malic enzyme and 6-phosphogluconate dehydrogenase genes) has been studied. DNA-RNA hybridization has shown there is an inverse correlation between the level of DNA methylation and gene expression of malic enzyme and 6-phosphogluconate dehydrogenase. Thus it suggests that the regulation of thyroid hormone gene expression can take place via DNA methylation blocking and that DNA demethylation is part of structural changes essential to the binding of thyroid hormones with DNA elements recognized by thyroid hormone receptors and to further induction of synthesis of specific mRNA.

Animals↗
PubMed1993

[The regulation of the nuclear RNA polymerase activity of the liver and brain in rats of different ages by a cytoplasmic modulator of thyroxine action].

The effect of physiological, concentration of thyroxine and cytoplasm thyroxine-binding protein (thyroxine action modulator, TAM4) on the RNA-polymerase activity in rat liver and bain cells in ontogenesis was studied. The physiological doses of the hormone were shown not to effect the RNA-polymerase of isolated liver and brain nuclei, while TAM4 caused the stimulation of enzymatic activity in these organs. ++TAM4 stimulating effect in liver correlated with increasing animal age, while the maximum RNA-polymerase activation by tAM was discovered in young rats under one month-At later stage the RNA-polymerase activity was same as in the controls. In is suggested that the cytoplasmic hormone-binding proteins take part in the formation of liver and brain sensitivity to the action of the thyroid gland hormones.

Aging↗
PubMed1991

[Membrane reception of thyroid hormones].

Comparative and competitive analyses of thyroxine (T4) and triiodothyronine (T3) binding to highly purified rat liver, brain and lung cell plasma membranes were carried out. The dependence of hormone binding on the time, temperature and concentration was studied. The effects of trypsin and partial delipidation on the binding parameters of thyroid hormones were investigated. Two thyroid hormone-binding sites were detected in cell plasma membranes of all tissues under study. The maximal binding of T4 to rat liver membranes and the maximal binding of T3 to rat brain membranes was observed in all experiments, the affinity for T3 being higher than that for T4. An important role of both protein and lipid components of plasma membranes in the membrane reception of thyroid hormones is proposed.

Animals↗
PubMed1989

[Mapping of the thyroglobulin gene in high molecular weight DNA from the human thyroid in normal conditions and in thyroid pathology].

A highly purified thyroglobulin (Tg) mRNA was isolated from human nodal euthyroid goiter. Ultracentrifugation in a 5-20% sucrose density gradient revealed that the protein has a sedimentation coefficient of 33S. cDNA was synthesized from the 33S RNA, using reverse transcriptase in the presence of a human placental ribonuclease inhibitor. In order to detect the polymorphism of restriction fragment length, screening of high molecular weight DNA from normal human thyroid gland and from nodal euthyroid and diffuse toxic goiters was carried out, using Tg cDNA probes and plasmid DNA containing a Tg cDNA fragment. After restriction with endonuclease Hind III, three Tg-containing fragments were identified both in the normal and nodal euthyroid goiters, whereas restriction with endonuclease Bam HI revealed two Tg-containing fragments in nodal euthyroid and diffuse toxic goiters.

Animals↗
PubMed1989

[The effect of cryopreservation and transplantation factors on the morphofunctional state of the thyroid in dogs].

Influence of transplantation of non-specific factors (denervation, delymphatization, surgical trauma, ischemia, changes in blood supply conditions) as well as the effect of cryopreservation (-196 degrees C) on morphological structure and ability for secretion of thyroid hormones and response to TS stimulation were studied using the model of extracorporal biological perfusion in isolated thyroid of dogs. The results of biochemical and morphological investigations of thyroid in different conditions of perfusion showed, that it had preserved its main functional and morphological characteristics under the action of transplantation of nonspecific factors and extremely low temperatures.

Animals↗
PubMed1986

[Extracorporeal biological perfusion of isolated canine thyroid glands].

A model of extracorporeal biological perfusion of the isolated thyroid gland in dogs has been developed. The experiments on dogs were performed to analyse the functional state of the perfused gland. It has been shown that the functional state parameters in 6-hour perfusion were comparable to the control values. It is concluded that the model suggested creates physiologic conditions and can be employed as a model for studying the influence of various factors on the isolated thyroid gland, as well as for investigating problems related to thyroid gland transplantation.

Animals↗
PubMed1985

[Electrogenic transport of bicarbonate ions through the internal mitochondrial membrane induced by cytoplasmic glycopeptide].

Cytoplasmatic glycopeptide-induced bicarbonate ion electrogenic transport through mitochondrial membrane has been studied. Glycopeptide, described earlier as a factor increasing inner mitochondrial membrane permeability for phosphate ions, has been also shown to induce bicarbonate ion electrogenic transport through inner mitochondrial membrane. HCO3- ion transport, induced by cytoplasm glycopeptide is realized by pH-dependent pore. The interrelation of cytoplasm glycopeptide with mitochondria is regulated by mitochondrial energization.

Animals↗
PubMed1984

[Induction of electrogenic phosphate transport through the mitochondrial membrane by a thermostable cytoplasmic factor in hyperthyroidism].

Addition of a thermostable cytoplasmic fraction leads to the uncoupling of oxidative phosphorylation of the mitochondria. In hyperthyrosis such an effect manifests itself more powerfully than in the control. Addition of the thermostable cytoplasmic fraction induces electrogenic phosphate transport via the mitochondrial membrane. In hyperthyrosis, the activity of the thermostable inducer of phosphate transport in the cytoplasm increases. The functioning of the phosphate cycle may be the cause of the uncoupling of oxidative phosphorylation of the mitochondria during the disease in question.

Animals↗
PubMed1983

[The role of insulin-dependent cytoplasmic regulator in carbohydrate metabolism during immobilization].

Immobilization decreased the activity of insulin-dependent cytoplasmatic regulator in the rat liver. Cessation of the immobilization resulted in increment of insulin--dependent cytoplasmatic regulator activity in liver and diaphragm. After the immobilization the glycogen content is considerably higher in the liver, which seems to be due to an increase in the regulator activity. The restoration after the immobilization leads to a decrease in hypoglycemic action of insulin in vivo and in insulin-dependent cytoplasmatic regulator action in vitro.

Animals↗
PubMed1983

[Regulation of pyruvate transport in mitochondria by thyroid hormones].

During thyroidectomy, the stimulating action of the catalytic amounts of a thermostable fraction of rat liver and diaphragm cytoplasm on Ca2+ transport in mitochondria, which indicates the decrease of the activity of an insulin-dependent cytoplasmic regulator (IDR) in insulin target organs. Thyroidectomized rats also manifested a decrease in blood insulin and glucose concentrations. Administration of the physiological doses of thyroxine produced an increase in both blood glucose concentration and IDR activity in the liver and diaphragm of thyroidectomized rats. Experiments with measuring the kinetics of the swelling of deenergized mitochondria in isoosmotic solution of ammonium pyruvate demonstrated the inhibition of liver mitochondrial swelling in thyroidectomized rats.

Animals↗
PubMed1983

[Relation between the changes in glycolipid levels and substrate oxidation in skeletal muscle in alloxan diabetes in rats].

Administration of alloxan to rats in a dose of 50-150 mg/kg raises blood sugar level from 200 +/- 14 to 800 +/- +/- 16 mg/100 ml. Under these conditions, the content of total gangliosides in the rat femoral muscle progressively decreases, namely from 137 +/- 9 micrograms sialic acid per g tissue to 63 +/- 4, which is linked with alloxan diabetes severity and hyperglycemia. At the same time there was a 3-4-fold inhibition of 14C-glucose oxidation intensity by tissue sections of the skeletal muscles in the presence of a 1.5-2-fold activation of 1-14C-palmitate. Fractionation of glycolipids on silica gel KSK demonstrated a 33% decrease in the content of monosialogangliosides, and a lesser diminution in the content of other fractions. Total muscle gangliosides, added to the incubation medium exogenously, produced a pathogenetic correcting action on disturbed oxidation of 14C-glucose in alloxan diabetes.

Animals↗