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Biomedical subjects

I Watanabe

Publications and source records attributed to I Watanabe.

802 records · Page 45Linked to original sources
PubMedDate not supplied

Alkyllysophospholipid influenced melanoma cell morphology is associated with decreased attachment to basement membrane.

The alkyllysophospholipid analog 1-0-octadecyl-2-0-methyl-3-phosphorylcholine (ET-18-OCH3) was examined for possible anti attachment effects on B16-F10 murine melanoma cells in vitro. At sub-lethal lipid concentrations B16-F10 cells were inhibited from attaching to reconstituted basement membrane (Matrigel) during a 45 min assay. This type of inhibition was also imparted by the isoprenoid farnesol but not by egg lysophosphatidylcholine (LPC) at concentrations up to 10 micrograms/ml. Both lipids were toxic to B16-F10 cells in the absence of bovine serum albumin (BSA), BSA (0.1%) completely protected the cells from lysis except when both lipids were combined as a mixture. Light and electron microscopy, as well as electronic sizing of cells, gave evidence of alkyllysophospholipid induced reduction in cell size which correlated well with attachment inhibition. The results suggest that alkyllysophospholipid induced reduction of cell surface area leads to inhibition of cell attachment to basement membrane which 8 with our experimental conditions, was not permanent since cells eventually attach within 24 h after treatment. The enhanced lytic effect the lysophospholipid imparts on the alkyl compound, in conjunction with the anti-attachment properties should be important areas for future research.

Animals↗
PubMedDate not supplied

Function of the eustachian tube in an alternobaric environment. A study with special reference to the therapy of secretory otitis media in children.

The authors have obtained interesting data from the alternobaric treatment of secretory otitis media in children. Some functional differences of the Eustachian tube between adults and children were then studied by the combined use of sonotubometry and pressure loading. The results showed speculated that Eustachian tube function changes from infant type to adult type between the ages of 6 and 10 years.

Atmospheric Pressure↗
PubMedDate not supplied

Development of mouse submandibular gland studied by field emission scanning electron microscopy.

The ultrastructural organization of mouse submandibular gland from 15 days gestation to 180 days of age was studied by field emission scanning electron microscopy. At 15 days of gestation several groups of acini, intercalated and striated ducts were present. They consisted of numerous pyramidal and/or polyhedral cells with spherical or elongated nuclei and short microvilli. At 3 days after birth different shapes of acinar structures covered by a network of fine collagen bundles were observed. The acinar portions corresponded to the terminal tubules and showed the acinar cells containing immature secretory granules. Fractured specimens of ducts revealed a flat surface with large central nuclei. At 14 days after birth acinar terminal portions possessed a round shape; branches of either intercalated and striated ducts were also observed. Gap junctions and interdigitations were numerous at the base of acinar cells. At 30 days after birth the acinar terminal portions and striated ducts revealed a large number of secretory granules. Acinar cells showed a pyramidal shape and basal nuclei. Freeze-cracked surfaces of the striated ductal cells evidenced also a pyramidal shape. Secretory granules ranged from 0.3 to 1.2 microns in size and were clearly observed by infoldings of the basement membranes. At 90 days after birth the ultrastructural features were more differentiated when compared with the previous ages. The freeze-cracked specimens showed very numerous secretory granules in all acinar and striated ductal cells. The granules occupied almost all the apical region of the cells. The outer surface of the basement membrane of acinar and myoepithelial cells was constituted by a spongy-like material covered by fine collagen fibrils. At 180 days after birth numerous secretory granules were seen either in acinar and ductal cells. A morphofunctional polarization of the cell was finally clearly observed in that the cytoplasmic organelles were concentrated in the basal portion whereas the secretory granules were located in the apical region of the cells.

Animals↗
PubMedDate not supplied

A simple method for evaluating masticatory performance using a color-changeable chewing gum.

PURPOSE: This study investigated the usefulness of a newly developed color-changeable chewing gum for evaluating masticatory performance. MATERIALS AND METHODS: The color changes of the gum after chewing were measured using the L*a*b* color space defined by the Commission Internationale de l'Eclairage with a spectrophotometer. Numerical scores and a color scale were developed to easily evaluate color change. Numerical scores were assigned corresponding to the degree of color change, and a color scale was made by using the numerical scores. Four examiners graded the gum chewed by 18 fully dentate subjects by using the color scale. RESULTS: As the number of chews increased, the degree of color change increased significantly. The percentages of correct answers by examiners using the color scale method ranged from 67% to 83%. CONCLUSION: The results indicate that this simple method may be used to quantitatively evaluate masticatory performance and may be reliable enough to be used clinically.

Adult↗