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Biomedical subjects

I Walewska

Publications and source records attributed to I Walewska.

At least 19 recordsLinked to original sources
PubMed1991

[Value of using polyethylene glycol in the indirect antiglobulin test with erythrocytes].

A series of comparative investigations was carried out for establishing the value of the indirect antiglobulin test (IAT) in its first phase. The results showed that PEG, in relation to NaCl solution of low ionic strength, increased the reactivity of various antibodies of different specificity present in 72 studied sera. A significant superiority of IAT-PEG to IAT-BISS was noted in 45.8% of these sera. In search for antibodies in sera of 1160 patients IAT-PEG detected 7 antibodies not reacting in the IAT-LISS, and 5 of them had Rh system specificity, one was anti-K and one anti-Jka. Although the IAT-PEG was more sensitive than IAT-LISS in the detection of anti-Rh antibodies, it was less sensitive in this range than the enzymatic LEN test with which not only 5 of these antibodies were demonstrated but which detected also 8 antibodies not demonstrated in IAT-PEG or IAT-LISS. All 5 antibodies not detected by the IAT-PEG but demonstrated in the IAT-LISS were specific for the Lewis system. The per cent of reactions regarded as non-specific in the IAT-PEG was 1.3%. The IAT-PEG modification may be recommended as a sensitive, simple and specific method extending the possibilities of immunohematological diagnosis, especially in cases of post-transfusion reactions and in difficulties of interpretation connected with the occurrence of weakly positive reactions in the IAT-LISS.

Blood Group Antigens↗
PubMed1976

[Stimulation of secondary immune response to antigen D from the Rh system].

The authors report the results of investigations on stimulation of secondary immunological response in 30 subjects immunized with antigen D for obtaining highly active sera for production of anti-D immunoglobulin. The analysis of these results showed that application of frequent antigenic stimuli leads to a short-term rise in the antibody level but in 10 cases no expected effect was obtained. None of the immunized subjects produced serologically detectable antibodies reacting with platelets.

Blood Platelets↗
PubMedDate not supplied

Analysis of immune response to red blood cell antigens in multitransfused patients with different diseases.

The rate of alloimmunization to red blood cell antigens in 1502 multitransfused patients, mainly with blood disorders, was analyzed in a retrospective study. The overall incidence of alloantibodies was 5.7%. Three groups of patients were identified with different potential for antibody production. The lowest probability (1.8%) of alloimmunization was found in the group of patients with lymphoproliferative syndromes, acute myeloid leukaemia and burn disease. The highest probability (33.4%) of immune response to red blood cell antigens was found in patients with AIHA, liver cirrhosis and myelodysplastic syndrome. In the group of patients with chronic myeloid leukaemia, pancytopenias, anaemias of various origin and aplastic anaemia the probability of alloimmunization ranged from 5.7% to 13.6%. A possible role of genetic-factors and immune competence status in post-transfusion alloimmunization is briefly discussed.

Adolescent↗
PubMedDate not supplied

[Remarks on routine use of the enzyme test LEN (LISS Enzyme) in immunohematological studies].

The authors report one-year experience with the enzymatic test LEN (LISS-Enzym) replacing the one-step papain test in the compatibility testing, and in place of the two-step papain test in the detection and identification of red cells allo-antibodies. A wide range of investigations confirmed the usefulness of this test in the detection and antibodies specificity, mainly from the Rh system which are often undetectable by means of the indirect antiglobulin tests. It is concluded that in compatibility tests the LEN test should replace the low-sensitivity one-step papain test used as yet. It is stressed that in case of positive reactions only in the LEN test suggesting presence of autoantibodies the test should be repeated bringing to 37 degrees C the reacting components before their mixing. This makes possible elimination of clinically not significant cold autoantibodies.

Diagnostic Tests, Routine↗
PubMedDate not supplied

[Value of the LEN (LISS-enzyme) technic in detection of alloantibodies to erythrocytes].

The reported study was done for assessment of the value of the enzymatic technique LSE/LISS Spin Enzyme proposed by Odell et al. using a solution of NaCl of low ionic strength (LISS) for detection of incomplete alloantibodies to erythrocytes, especially those from the Rh system. The results of the study demonstrated that the LSE test, called by the authors LEN (LISS-Enzyme) is a sensitive and specific method comparable in its effectiveness to the two-step papain test (2-Pap) used widely in this country. In routine studies the LEN technique is simple, easy and rapid. Washing of papain treated erythrocytes is not necessary. The time from enzyme addition to erythrocytes to result reading is about 15 minutes, that is it is four times shorter than the time in the 2-Pap technique (60 minutes). A significant was the preparation of one type of erythrocyte suspension in LISS solution for two tests: enzymatic and antiglobulin, carried out in parallel. For these reasons the LEN test may be recommended in place of 2-Pap for detection and identification of antibodies. It is also a practical methods for matching test in place of the one-step papain test. The LEN test should be widely introduced in laboratories of transfusiological immunology in this country. Attention is called ti various technical details necessary for obtaining of unequivocal and reproducible results.

Erythrocytes↗