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Biomedical subjects

I V Golubeva

Publications and source records attributed to I V Golubeva.

At least 19 recordsLinked to original sources
PubMed1992

[The clinico-biological aspects of transsexualism].

A complex constitutional-endocrinological investigation of persons of both sexes with clinical diagnosis of transsexualism (TS) has been held. On the basis of original data some peculiarities of somato- and cerebrotypes of transsexuals have been revealed, the formation of these peculiarities is significantly connected with the action of sexual hormones. A disturbance in ratio of male and female sexual hormones in the direction of predominance of the hormones corresponding to the patient's sexual autoidentification despite their biological sex has been marked. It gives possibility to consider that TS pathogenesis is undoubtedly connected with inborn pathology of hormonal homeostasis. The results of multi-disciplinary approach to study of TS are being discussed. An assumption about the role of disturbance in the system of "hormones-brain" in the TS origin is given.

Acupuncture Points↗
PubMed1988

[The demand for the main cardiovascular preparations in the city of Frunze].

The structure of consumption and need in cardiovascular drugs were studied in a hospital and in the Kirghiz Scientific Research Institute of Cardiology according to a scheme developed in the USSR Cardiology Research Center. Use of cardiovascular drugs was studied with respect to optimal results of therapy in 361 patients with main cardiac pathology (ischemic heart disease, essential hypertension and their combination) who had received 1125 courses of drug treatment. The prescriptions were analysed according to their intensity, regularity and optimal effect. The authors assessed provision of cardiological patients of Frunze with drugs and gave recommendations on its improvement, in particular, satisfaction of need in some active drugs (prolonged nitrates, calcium antagonists, cardiac glycosides).

Cardiovascular Agents↗
PubMed1984

[Escherichia coli with the K1 antigen isolated in pyelonephritis in children].

The identification of K antigens in 113 E. coli strains isolated from the urine of 62 pyelonephritis patients was carried out. 84 strains were found to possess K antigens. The presence of K antigens was revealed in strains both in the S-form (67 cultures) and in the R-form (17 cultures). Among the strains with K antigens the presence of K1 antigen was revealed in 41.7 +/- 6.5% of cases. K1 antigen was found in combination with eight groups of O antigens (01, 02, 04, 017, 018, 050, 077, 0141) and with R antigens. The above-mentioned E. coli strains were isolated from 16 patients; of these, 3 patients had chronic pyelonephritis.

Adolescent↗
PubMed1983

[Determination of the formation of hydrogen sulfide in a dry differentiating medium of the Kligler agar type].

The indicator system for hydrogen sulfide detection in media analogous to Kligler's agar, intended for the primary identification of enterobacteria, has been studied. The indicator system under study, in contrast to the known formulas, contains sodium metabisulfite for improving the capacity for detecting hydrogen sulfide production in bacteria which produce it in small amounts from inorganic sulfur compounds.

Agar↗
PubMed1981

[Serologic groups of O-antigens of E. coli isolated from urinary tract infections within the boundaries of the USSR and East Germany].

A total of 373 E. coli strains, isolated in urinary tract infections from children and adults in Rostock (GDR) and Moscow (USSR), were found to belong to 63 O-groups. E. coli isolated in Rostock belonged to 36 O-groups, and in Moscow to 53 O-groups. The occurrence of E. coli serogroups in the dominating O-groups (more than 2% of the total number of strains) in Rostock (O1, O2, O4, O6, O7, O8, O9, O15, O21, O25, O75, O102) differed from that in Moscow (O1, O2, O4, O6, O8, O9, O15, O16, O17, O18, O25, O28, O73, O75) in composition and frequency, in Rostock the predominant E. coli groups being O2, O4, O6, O7, O9, O21, O75, O102, while in Moscow O1, O8, O15, O16, O17, O18, O25, O28, O73. The partial O-antigen composition of E. coli strains belonging to the same O-groups, isolated in Rostock and Moscow, was different in groups O1, O2, O125.

Antigens, Bacterial↗
PubMed1981

[Evaluation of a complex method of identifying enteropathogenic Escherichia using O- and H-sera and a rational combination of biochemical tests].

A complex method for the identification of enteropathogenic Escherichia by means of adsorbed monogroup and factor O-sera, H-sera and a rational combination of biochemical tests (using triple sugar iron agar or Kligler iron agar, Simmons citrate agar, phenylalanine-, urea-, sodium malonate-containing media, indole broth, medium for mobility determination) was evaluated. The use of adsorbed monogroup O-sera in the slide agglutination test reduced the time of the study and increased its accuracy by 20.0 +/- 2.5% (6.9 +/- 2.5% to 52.4 +/- 11.1% for Escherichia of different O-groups) in comparison with the generally accepted method. The use factor O-sera and H-sera and H-sera revealed the circulation of the same O-groups belonging to different serovars and differing in partial composition a O-antigen (of the type ab - ac) and in H-antigens, which should be taken into consideration for epidemiological purposes. The combination of biochemical tests ensured correct determination of the genus of the organisms under study. The tested method is recommended for practical use.

Escherichia↗
PubMed1978

[Isolation of bacteria of the Klebsiella genus in diarrhea in polar researchers].

Representatives of Klebsiellae tribe (K. pneumoniae, Ent. cloacae, Ent, aerogenes) were isolated in examination of pharyngeal discharge and feces of members of the polar expedition suffering from diarrhea and their contacts; the same microbes were revealed in the antarctic lake which served as the source of drinking water. The mentioned bacteria possessed typical cultural and morphological, as well as biochemical properties. K34 serovar was determined in serological identification of Kl. pneumonia. At the same time it was demonstrated that Klebsiella--representatives of Enterobacteriaceae could be facultative psychophils and persist in the external environment under low temperature conditions; entering human organism they could cause diarrhea, i.e. could act as pathogens.

Cold Climate↗
PubMed1977

Obtaining hyperimmune anti-coli (anti-Escherichia) human plasma.

Anticoli (anti-Escherichia) plasma has been obtained from donors immunized with viable Escherichia oral vaccines, prepared from Str-d mutants of E. coli. Anticoli plasma has an therapeutic effect against diseases of Escherichia etiology.

Antibodies, Bacterial↗
PubMed1976

[Temperature-dependent mutants of Hly plasmid and their use for the purpose of confirming the cytotoxic activity of E. coli hemolysin].

Two different mutants of Hly 212 plasmide induced by diethyl sulfate were isolated; one of these was characterized by temperature-dependent synthesis, and the other--by temperature-sensitivity of hemolysin produced. In difference from bacteria possessing wild parental plasmide, E. coli K12 bacteria which contained the mutants produced no cytotoxic action on the MK2 cell culture. This correlated with the absence of the hemolytic activity in growing them even at permissible temperature on medium No 199. After crossing the mutant plasmides there were obtained clones with completely restored wild phenotype of the hemolysin production and the cytotoxic activity. The data obtained pointed to the relationship between the cytotoxicity and hemolysin.

Cytotoxicity Tests, Immunologic↗
PubMed1976

[A new variant, for the USSR, of enteropathogenic escherichia 0151:K--, isolated from acute intestinal diseases under the conditional name "Crimea"].

A study was made of enteropathogenic escherichia "Krym" isolated in the USSR from patients with acute enteric diseases; their antigenic structure was unknown. Also cultures of new serological groups officially recorded as standard test-strains were investigated. By its antigenic structure "Krym" escherichia corresponded to the standard strain of the international set 0151:K--. Along with the H10 antigen described earlier "Krym" escherichia proved to possess H11 antigen; these two serological types of escherichia were revealed in the USSR, i.e. 0151:K--: H10 and 0151:K--: H11. The statement on the presence of a new H50 antigen described in escherichia 880-67 with an antigenic formula of 0151:K--: H50 should be revised due to its complex identity with the 0151: K--: H10 strain. For proper serological identification the conditioned name "Krym" should be replaced by "escherichia of serological group 0151:K--".

Acute Disease↗
PubMed1976

[Biological characteristics of enteropathogenic escherichia of serologic group 015:K].

A study was made of 239 strains of enteropathogenic escherichia 0151:K-- isolated in various regions of the USSR from patients with the clinical diagnosis of dysentery, gastroenteritis, intestinal coli-infection: a standard strain of the international collection of escherichia belonging to the given serological group was also studied. There was shown an increase in the role of these microorganisms among the enteropathogenic escherichia recorded at the territory of the USSR; they occupied the third place by the frequency of isolation after the serological group 0124:K72 and 0111:K58. There was established a common nature of the enzymatic characteristics of escherichia 0151:K--with shigellae by the absence of lactose, sucrose, inosite, adonite fermentation, the presence of gasless, immobile variants containing no lysin decarboxylase, and a possibility of rapid differentiation from shigellae in the use of acetate medium. Among the escherichia 0151:K--there was revealed the presence of 5 biotypes by the capacity to gas-formation in glucose, arabinose, sorbit, dulcit fermentation, and decarboxylation of lysin and ornithin; three biotypes are described for the first time. Industrial issue of the agglutinating serum 0151:K--is necessary to provide the diagnosis of these microorganisms at the territory of the USSR.

Escherichia coli↗
PubMed1975

[Data on the biological characteristics of serological group O4 Escherichia isolated in acute intestinal diseases].

A study was made of 155 strains of E. coli of the O4 serological group isolated from sick children and adults during group and sporadic acute intestinal diseases), from persons who came in contact with them, and also from healthy persons during prophylactic examination; three standard cultures were examined as well. Along with strains with a typical enzymatic activity there were strains which produced retarded lactose fermentation and also gas-free, immobile and lysin-negative strains resembling Shigellae. Eight biochemical types were determined among E. coli 04. A study of the antigenic structure by cross agglutinin adsorption indicated identicity of the strains by O-antigen and their difference by the K- and H-antigens. Circulation of E. coli of serological types O4: K12(L): H1,O4: K3(L): H5,O4: K3(L): H12,04: K12(L): H40,04: K52(L): H4, and O4: K12(L) HII was revealed; the first two serological types prevailed. Serological types of O4: K3(L):H12, O4: K12(L): HI and O4: K12(L): H40, isolated in cases of group and sporadic acute intestinal diseases were described for the first time.

Acute Disease↗