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Biomedical subjects

I Tsuda

Publications and source records attributed to I Tsuda.

71 records · Page 4Linked to original sources
PubMed1985

Evaluation of a new blood cell counter with sheath flow system.

A blood cell counter with a sheath flow system has been developed to eliminate the drawback of the Coulter type blood cell counter, namely, signal distortion caused by the cells passing through the electric aperture gate. With the new system, signal distortion was low and a near-normal distribution curve for erythrocytes and resin particles was obtained. The counter has a computer program for determining the red cell distribution width, which represents an actual size difference at 20% of the relative frequency of the distribution curve. This is independent of mean cell volume, and is considered to be of clinical importance. We examined the values in 2,300 healthy subjects and patients with various hematological disorders.

Blood Cell Count↗
PubMed1985

Sickle cells show low expansibility and high resistance to saponin.

The expansibility under hypotonic condition and the susceptibility to saponin under hypotonic and deoxygenated conditions were determined. The experiments were carried out using a conventional blood cell counter and comparable volume changes were displayed on a chart as a graphic presentation. The sickle cells showed low expansibility and strong resistance to saponin, where the resultant curve of sickle cells showed prolonged hemolysis time and characteristic volume change. Thus, the methods would be useful for detecting sickle cell disease during routine laboratory procedures.

Anemia, Sickle Cell↗
PubMed1984

Involvement of contractile protein in hypotonic expansion of macrophages.

The volume measurement of macrophages from rabbit lungs was carried out using an automatic computerized cell counter. The size of freshly prepared macrophages was 669 +/- 62 fl at 4 degrees C. The cell volume increased gradually when the cells were suspended in an isotonic saline solution. Cytochalasin B initially enhanced the increase in cell volume but then caused a reduction. The reduction was considered to be due to structural changes in filament network of contractile proteins. Under hypotonic conditions, cytochalasin B-treated cells showed increased osmotic fragility and reduced expansibility. The increased fragility was considered to result from impairment of the network structure. These findings of the effect of cytochalasin B on the volume change suggest that contractile proteins play a protective role in the membrane against hypotonic expansion.

Animals↗
PubMed1984

Automated continuous measurement of erythrocyte volume under isotonic conditions.

The time course of volume changes of erythrocytes, suspended in isotonic solutions containing permeable substances, was studied using a cell monitoring system which was assembled on the basis of Coulter's principle. For comparison, the hematocrit method was employed. The data obtained demonstrated that urea, glycerol, ethanol, and methanol induced a rapid volume increase, whereas glucose and galactose induced a slow volume increase. These results correspond well to the data obtained by the hematocrit method. The present method, which requires only a minimum volume of blood, showed high accuracy and high reproducibility. Thus, the method was considered applicable to experimental and clinical studies of membrane permeability of erythrocytes.

Cell Membrane Permeability↗
PubMedDate not supplied

Maturation of Howell-Jolly bodies observed in a case of malignant histiocytosis.

The maturation of Howell-Jolly bodies was microscopically observed on the May-Grünwald-Giemsa-stained film of bone marrow obtained from a patient with malignant histiocytosis. The bodies separated from the nucleus at the polychromatophilic normoblast stage and condensed faster than the main nucleus before denucleation in the normoblast stage.

Adult↗