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Biomedical subjects

I Tasaki

Publications and source records attributed to I Tasaki.

134 records · Page 8Linked to original sources
PubMed1965

Electrophysiological studies of chilean squid axons under internal perfusion with sodium-rich media.

Electrophysiological properties of giant axons of Chilean squid were examined by intracellular perfusion combined with various recording techniques. The amplitude of the action potential of the axons perfused with equal sodium concentrations (0.35M) on both sides of the membrane was approximately 75 millivolts (glutamate salts being used internally); the resting potential recorded with a calomel electrode was roughly 45 mv. These observations confirm and extend previous data, obtained from Chilean squid axons, which show that the amplitude of the action-potential overshoot does not agree with the Nernst equation applied to sodium ion. The discrepancy between these results and those obtained by others is attributed to differences in the procedures used to determine the resting potential.

Animals↗
PubMed1965

Effects of internal and external ionic environment on excitability of squid giant axon. A macromolecular approach.

The effects of ten cations and fifteen anions on the excitability of the squid giant axon were studied. The method of intracellular perfusion used in these investigations is described in detail. Empirical criteria were established for evaluating the relative favorability of any salt solution for maintaining the normal excitability of the membrane of the axon. It was found that both cations and anions could be ordered in sequences of relative favorability, which are directly related to the classic lyotropic sequences found for protein macromolecules and in colloid chemistry in general. The effects of concentration, salt mixtures, non-electrolyte carriers, enzymes, metabolic inhibitors, pH, and external media were also studied. The results are interpreted in terms of current concepts of the interactions between water structure, charged macromolecules, and their ionic environments. A macromolecular approach is given to the physicochemical nature of the "two stable states" of the excitable membrane, to describe the time-dependent potential changes observed.

Animals↗