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Biomedical subjects

I Tasaki

Publications and source records attributed to I Tasaki.

At least 109 records · Page 6Linked to original sources
PubMed1971

Properties of squid axon membrane as revealed by a hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate.

A hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS), was introduced into the interior of a squid giant axon and fluorescene properties of the TNS-stained axon were examined under illumination with polarized ultraviolet light. A transient reduction in fluorescence was observed when the axon was stimulated electrically. The light waves contributing to this fluorescence change were found to be nearly completely polarized, indicating that the probe molecules in the membrane are highly oriented. The emission spectra of these TNS-stained axons were examined at rest and during nerve excitation. The results obtained are considered to support the view that the process of nerve excitation is accompanied by a transition of the axon membrane from a hydrophobic to a hydrophilic state.

Animals↗
PubMed1970

Changes in extrinsic fluorescence in squid axons during voltage-clamp.

Fluorescence changes in squid axons were examined after staining with rhodamine B, pyronin B, or 8-anilinonaphthalene-1-sulfonate by intracellular application. Gradual changes in fluorescence were detected during both hyperpolarizing and depolarizing voltage-clamp pulses. Abrupt changes were often observed at the onset and at the end of voltage-clamp. Possible sources of artifact in optical measurements of this type and some implications of the findings are discussed.

Animals↗
PubMed1969

Fluorescence changes during conduction in nerves stained with Acridine Orange.

Nerves from spider crabs anid squid fluoresce when staiined with Acridine Orange. The intenisity of fluorescence increases during nerse conduction. Prolongation of the electric response in the squid axon is associated with a fluorescence change of similar duration. These findings suggest that the physicochemical properties of the macromolecules around the dye molecules in the nerve membrane drastically change during the process of nerve conduction.

Acridines↗