Biomedical subjects
Publications and source records attributed to I Takeda.
Metaplastic bony tissue along with hyperplastic mucosal epithelium showing no atypism was detected in biopsy materials from a Yamada type I gastric polyp. The tissue was metaplastic woven bone associated with calcification. Histogenesis of the bone formation is as yet unknown. This is the first reported case of the presence of metaplastic bone accompanied by hyperplastic gastric mucosa so far.
We have performed a double immunofluorescent staining of two monoclonal antibodies, obtained from gastric cancer patients, that were directed against lymphocyte surface antigens on the regional lymph nodes. Through this method we were able to identify the lymphoid cell subset in detail. As a result of our double staining, most OKT8+ lymphocytes were also found to be Leu 15- lymphocytes and these OKT8+ Leu 15- lymphocytes were regarded as being cytotoxic effector cells or precursors. A few OKT8+ Leu 15+ lymphocytes (suppressor T lymphocytes) were obtained. More Leu 3a+ Leu 8- lymphocytes (helper T lymphocytes) were observed than Leu 3a+ Leu 8- lymphocytes (suppressor inducer T lymphocytes). To investigate whether these T lymphocytes were activated, we used an OKT 9 monoclonal antibody directed against the transferrin receptor and an OKIa1 monoclonal antibody directed against the HLA-DR framework. Such double stained lymphocytes (activated T lymphocytes) were few.
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The index of the baroreceptor reflex sensitivity (BRS) was measured noninvasively by monitoring the arterial pressure (AP) of the finger and R-R interval on the ECG during a Valsalva maneuver. An indirect finger AP measurement system was newly designed on the basis of the so-called vascular unloading technique. The BRS index was determined as the slope of the linear regression equation between the R-R interval and the virtual brachial AP which was evaluated from the finger AP. The data on both parameters were taken from the second period of the fourth phase in the Valsalva maneuver. The measurement was carried out on 24 inpatients (29-65 years) with essential hypertension. The conventional invasive method using phenylephrine or nitroglycerine was also performed on the same subjects. The correlation coefficient between the invasive and noninvasive methods was 0.81 when phenylephrine was used and 0.90 with nitroglycerine (p less than 0.001). The present method was found useful in routine clinical examination because of its easy and repeatable noninvasive application to patients without causing stress.
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Human adult peripheral blood lymphocytes were successfully immortalized by co-cultivation with irradiated autologous and homologous T-cell lines harbouring human T-cell leukaemia virus-I (HTLV-I). The efficiency of transformation was the same in both cases. The participation of alloantigen stimulation in co-cultivation procedures is discussed, and it is stressed that factors other than alloantigen stimulation might be required for the efficient immortalization of human T-lymphocytes in vitro by HTLV.
The cross-reactivity of antibodies to adult T-cell leukemia (ATL)-associated antigens (ATLA) in human and monkey sera was investigated by indirect immunoperoxidase and immunoferritin methods using a human cell line (MT-2) carrying a type C virus (HTLV), two monkey cell lines (Si-1 and Si-3) carrying HTLV, and a monkey cell line (Si-2) carrying a type C virus isolated from an anti-ATLA-positive monkey. Anti-ATLA-positive but not-negative human and monkey sera gave positive immunoperoxidase reaction with all four virus-positive cell lines when studied by light microscopy. Electron microscopic findings revealed ferritin or peroxidase labeling of virus particles and plasma membranes of these four cell lines with antibody-positive but not-negative human and monkey sera. These results clearly indicate the cross-reactivity of anti-ATLA antibodies in human and monkey sera at light and electron microscopic levels, and the presence of antigenic determinants common to the surface of type C virus particles of human and monkey origin.
Annulate lamellae (AL) were observed in only three out of 40 cases of human prostatic carcinoma, but not in 20 cases of benign prostatic hyperplasia and 11 cases of presumably normal prostatic tissues. AL showed the continuity with the rough endoplasmic reticulum and seemingly the nuclear membrane consisting of lamellar or concentric arrangement of stacked membranes and occasionally annular structures. In addition, annuli were detected in the rough endoplasmic reticulum near the stacked membranes which were devoid of ribosomal attachment. These results disclosed that in human prostatic tissues, AL could be only rarely detected in actively dividing cancer cells, and were seemingly the temporary transitional structures transforming from the nuclear membrane to the rough endoplasmic reticulum.
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Rabbits were successfully infected with human T-cell leukemia virus type I (HTLV-I) and produced antibodies to adult T-cell leukemia-associated antigens (ATLA) on intravenous inoculation of the HTLV-producing human cord T-cell line MT-2, or autologous cell lines established by cocultivation with MT-2 cells. Lymphocytes taken from the rabbits between 4 and 14 days after the inoculation of MT-2 cells, but not lymphocytes obtained in earlier or later periods, could be immortalized in vitro and expressed ATLA and type C virus particles. However, lymphocytes harvested during an early culture period (5 to 8 days) were found to be negative for ATLA. The transformed cells had the karyotype of a normal male rabbit. Two rabbits inoculated with the autologous HTLV-producing cell lines did not allow their growth in vivo, but some peripheral blood lymphocytes could be immortalized in vitro. One of these transformed cell lines was examined for the integration of HTLV-I provirus genome. The transformed cells were found to contain the provirus genome and also to be monoclonal with respect to the integration site of the provirus genome, unlike the inoculated cells.
Fasting serum bile acid (FSBA) was serially measured by a fluorescent enzyme method in a follow-up study of 61 patients with chronic liver disease. In chronic inactive hepatitis, fluctuation of FSBA was within the normal range in both the exacerbated state and in remission. In chronic active hepatitis, FSBA was abnormally elevated in both states, but the difference was not significant. In chronic active hepatitis where FSBA was elevated in the remission state above its value in the exacerbated state, exacerbation of the disease occurred repeatedly during the follow-up period. In compensated liver cirrhosis progressing into the decompensated form, FSBA levels increased before a decrease in the serum values of albumin, cholesterol, and cholinesterase, and an elevation of bilirubin. In liver cirrhosis, FSBA levels increased above 100 microM, 1-4 months before the appearance of ascites.
A case of poorly differentiated stromal tumor occurring in the left testis of a 37-year-old man is reported. Microscopically, the tumor was composed of polygonal cells arranged in a sheet-like pattern. The ill-defined cytoplasm contained disease-resistant PAS positive granules. Ultrastructurally, peculiar crystalloid structures were found in the cytoplasm of the tumor cells appearing in tubular, beaded or lamellar form and connected with the nuclear membrane as well as with the rough endoplasmic reticulum. These are somewhat similar to annulate lamellae. Interdigitation of the plasma membranes and zonula adherens was observed. Basal laminae were recognized in parts. These findings suggest that this tumor is a case of poorly differentiated gonadal stromal tumor showing a tendency to differentiate into Sertoli's cells.
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With the advancement of dialysis techniques, rehabilitation and high morbidity are becoming serious problems for patients with chronic renal failure. The authors, with the purpose of investigating the carcinogenic state of such patients, studied the incidence of malignant neoplasms and their immunity both clinically and fundamentally. Out of 499 patients with chronic renal failure, malignant neoplasms were found in 17 cases (3.40%), which is obviously a high rate with a statistically significant difference from that of the ordinary healthy population. In the fundamental immunity study, OKM1+ cells of the patients were significantly lower than those of healthy people, and a decrease in NK and K cells was inferred. Incidentally, the lymphocytes of patients strongly inhibited the mitogen response of healthy subjects. This decline in cellular immunity is considered to be closely related to the carcinogenicity of patients with chronic renal failure.
To search for lymphocyte marker antigens on the surface of human T-cell leukemia virus (HTLV), an immunoelectron microscopic study was performed on a HTLV-producing human T-cell line, MT-2, using monoclonal antibodies, such as anti-Leu-1, -Leu-2b, -Leu-3a, -Leu-5, -Leu-10 and -HLA-DR and OKIal. The reactivity of each antibody with MT-2 cells was tested by the immunoperoxidase method at the light microscopic level. OKIal, anti-HLA-DR and -Leu-10 gave positive results. At the ultrastructural level, the surface of HTLV as well as the plasma membranes of MT-2 cells were labeled with ferritin by the monoclonal antibodies OKIal, anti-HLA-DR and -Leu-10, but not by anti-Leu-1 and -Leu-3a. These findings suggest that HLA-D region -associated antigens are common antigenic determinants shared by the surface of HTLV and the plasma membranes of MT-2 cells. These antigens on the virus surface are probably picked up selectively from the plasma membranes and may play an important role in the interaction of HTLV and target T-cells.