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Biomedical subjects

I Stewart

Publications and source records attributed to I Stewart.

At least 73 records · Page 4Linked to original sources
PubMed1989

Renal metastases shown by 99mTc-methylene diphosphonate scintigram which failed to detect vertebral metastases.

A patient with breast metastases is presented showing increased nodular renal uptake of tracer on bone scintigraphy that also demonstrated rib metastases and a large metastasis in the pelvis. However, vertebral body metastases were not shown when clearly demonstrated on CT with even partial compression on a thoracic spine film. Most of the renal lesions were hypoechoic on sonography, showed hypoattenuation areas on plain CT and after contrast enhancement but no calcification could be demonstrated. This case once again illustrates the value of scrutinizing the soft tissues on scintigraphy but also the possibility of showing some metastases on CT not visible on scintigraphy.

Adult↗
PubMed1988

Migration of granulated metrial gland cells from cultured explants of mouse metrial gland tissue.

The migration of granulated metrial gland (GMG) cells from cultured explants of metrial gland tissue obtained from mice killed between days 10 and 16 of pregnancy has been studied. GMG cells migrated from all of the explants but more GMG cells were found around explants obtained from mice at day 10 of pregnancy than around explants obtained at later stages of pregnancy. The number of GMG cells found around each explant reached a peak at days 1, 2 or 3 of culture but only a few GMG cells were found around the explants by day 7 of culture.

Animals↗
PubMed1988

Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--II. Comparative aspects of 2-deoxy-D-glucose catabolism in opossum and human red cells in vitro.

1. G-6-P, F-6-P, F-1, 6-P, DHAP and GA-3-P in opossum erythrocytes were found at levels above those reported in human red cells. 2. About 1% of the radioactivity provided as [1-14C] DOG to red cells of both species was recovered as 14CO2 in 1 hr. 3. Unlike [1-14C] DOG, radiochromatography of extracts of cells incubated DOG revealed two diffusible radiolabelled compounds in the supernatant of cell suspensions. 4. The catabolism of DOG was quantitatively and qualitatively similar in opossum and human erythrocytes under the conditions of this study.

Animals↗
PubMed1988

Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--III. Metabolic depletion with 2-deoxyglucose markedly accelerates methemoglobin reduction in opossum but not in human erythrocytes.

1. Glucose-depleted, nitrite-treated erythrocytes reduce ferriheme in vitro in an environment 100 mM to 2-deoxy-D-glucose at a rate of 2.4 microM/ml cells/hr (opossum) and 0.37 microM/ml cells/hr (human). 2. During the process of methemoglobin reduction the breakdown of adenine ribonucleotides is more rapid in opossum (0.9 microM/g hg/hr) than in human (0.36 microM/g hg/hr) erythrocytes. 3. Radiolabelled ribose from [U-14C] ATP is catabolized exclusively to [14C] lactate in opossum, and to [14C] pyruvate and [14C] lactate in human red cells.

Adenosine Triphosphate↗
PubMed1988

Time course of the differentiation of granulated metrial gland cells in chimeric mice.

Granulated metrial gland (GMG) cell differentiation was examined in deciduomata in lethally irradiated mice which had been reconstituted with rat bone marrow. The time at which the bone marrow reconstitution was carried out was varied in relation to the time of initiating the decidual reaction. GMG cells were examined at various times after bone marrow transplantation to determine whether they had the morphology which characterised them as being derived from host or donor stem cells. Differentiation of donor type GMG cells was seen within the first week after transplantation and occurred even when the bone marrow was transplanted two days after initiating the decidual response.

Animals↗
PubMed1988

Granulated metrial gland cells in the non-traumatized regions of the uterus of ovariectomized mice with deciduomata maintained on progesterone.

A study has been made of granulated metrial gland (GMG) cells in the non-traumatized regions of the uterus of ovariectomized mice maintained on progesterone and with deciduomata in part of one uterine horn. GMG cells were found in the non-traumatized region of the uterine horns with deciduomata for at least 8 days after induction of the decidual reaction. GMG cells were not found in the contralateral horns without deciduomata. Activation of endometrial stromal cells is characterized by a high mitotic index and, in mice given colchicine, numerous stromal cells were arrested in metaphase in the non-traumatized region of the uterine horns with deciduomata for at least 3 days after induction of the deciduomata. In uterine horns without deciduomata, stromal metaphases were few in number over the same period. A correlation was therefore found between the presence of GMG cells and large numbers of stromal cells in mitosis. There was also a correlation between the absence of GMG cells and small numbers of stromal cells in mitosis. These results are compatible with the hypothesis that the differentiation and maintenance of GMG cells are under the control of endometrial stromal cells.

Animals↗
PubMed1988

The morphology of cultured cells from the mouse endometrium and decidua.

Single cell preparations were obtained from the endometrium of virgin and pseudopregnant mice as well as Day 5 and Day 8 deciduomata and were maintained in culture for periods of one to ten days. Three cell types, believed to be stromal cells, epithelial cells and macrophages, were identified in cultures prepared from virgin and Day 3 pseudopregnant uteri. Two further cell types, believed to represent mesometrial and antimesometrial decidual cells, were identified in cultures of cells from deciduomata. Granulated metrial gland cells were rarely seen, even in cultures of Day 8 deciduomata cells. Morphological changes in the cultured cells occurred with time and this was particularly evident in the cells believed to be derived from endometrial stromal cells and decidual cells. It is suggested that this represents in vitro growth of the cells which is independent of in vitro decidualization.

Animals↗
PubMed1987

The metrial gland.

The information available about the metrial gland of the pregnant rodent uterus with its content of granulated metrial gland (GMG) cells is reviewed. Recent research shows that GMG cells differentiate from bone marrow cells and supports the suggestion that GMG cells are involved in the immunological relationship between mother and foetus. There is probably a complex association between GMG cells and stromal cells of the metrial gland, and it is suggested that the association between GMG cells and the placental labyrinthine cells represents a functional interaction.

Animals↗
PubMed1987

Differentiation of granulated metrial gland cells in ovariectomized mice given ovarian hormones.

A study has been made of the differentiation of granulated metrial gland (GMG) cells in ovariectomized mice given progesterone and oestradiol at a time equivalent to the first 6 days of pregnancy. By varying the hormone regime, it was shown that progesterone is the ovarian hormone likely to be involved in stimulating GMG cell differentiation in the early pregnant uterus. Progesterone, however, failed to maintain, or allow further differentiation, of GMG cells during stages equivalent to the early post-implantation period, suggesting that the progesterone is not directly stimulating this cell population. The hypothesis that the differentiation and maintenance of GMG cells is under the control of activated stromal cells in the preimplantation period is supported by these results.

Animals↗
PubMed1986

Oestrogen and the differentiation of granulated metrial gland cells in chimeric mice.

The role of oestrogen in the recruitment of GMG cell precursors to the uterus has been investigated in radiation-induced chimeric mice reconstituted with rat bone marrow and maintained on a hormone regime simulating pseudopregnancy. The presence of rat GMG cells in the deciduomata in mice irradiated after oestrogen priming suggests that oestrogen priming is not an essential factor in recruiting GMG cell precursors. However, the results have led to the suggestion that in the normal, cycling mouse oestrogen may have a role in attracting GMG cell precursors to the uterus and preparing them for differentiation.

Animals↗
PubMed1985

Immunoglobulin G in the decidua basalis and metrial gland of the pregnant mouse uterus.

The distribution of immunoglobulin G (IgG) in the decidua basalis and metrial gland of the mouse uterus at days 10 and 14 of pregnancy has been investigated using immunohistochemical methods. IgG was found in the intercellular spaces but none was found in decidual cells, stromal cells of the metrial gland or granulated metrial gland cells. These results differ from those of other studies which have localised IgG in the cytoplasm of rat granulated metrial gland cells.

Animals↗
PubMed1985

Granulated metrial gland cells in the lungs of mice in pregnancy and pseudopregnancy.

A study has been made, using light microscopy, of GMG cells in the lungs of pregnant mice and pseudopregnant mice with deciduomata. GMG cells are found in the lungs from Day 8 to Day 17 of pregnancy and are mainly located in the interalveolar septa. The temporal relationship between GMG cells in the lungs and GMG cells in the blood vessels of the decidua basalis and metrial gland suggests that the GMG cells in the lungs are migrants from the uterus. The presence of GMG cells in the lungs is considered in relation to the possibility that a site of functional importance to this cell type is the labyrinthine placenta. GMG cells are found in the uterine blood vessels and lungs of pseudopregnant mice with deciduomata. This finding suggests that the migration of GMG cells is under the influence of the mother rather than under the control of factors derived from the fetal allograft.

Animals↗