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Biomedical subjects

I Smid

Publications and source records attributed to I Smid.

At least 19 recordsLinked to original sources
PubMed1994

Mycobacterial identification by computer-aided gas-liquid chromatography.

The suitability of the Microbial Identification System (MIS) marketed by Microbial ID (Newark, DE, USA) for routine diagnosis of clinically important mycobacteria was investigated and assessed. Cellular fatty acids were extracted from 1077 stock and recent clinical isolates. They were analyzed using a gas-liquid chromatograph combined with MIS software. The MIS system finally identifies the isolates by comparing their fatty-acid compositions with a standard library for mycobacteria. As the library search usually results in more than one possible match, suitable identification criteria were determined. The stricter these criteria are, the more the percentage of false-positive identifications can be reduced, but at the cost of more cases that remain undecided and require additional analysis. Under conditions similarity index (SI) SI1 > or = 0.4 and SI1-SI2 > or = 0.1, 63% of all isolates were correctly and 6% incorrectly identified.

Chromatography, Gas↗
PubMed1992

The use of radiometric tests in the speciation of mycobacteria--a review.

Besides a more rapid detection and susceptibility testing, radiometry offers possibilities for the identification of mycobacteria. This paper gives a chronological summary of radiometric techniques used in the BACTEC system (Becton Dickinson) for the identification of mycobacterial complexes/species, employing chemical compounds as selective inhibitory agents or for testing biochemical activities. Thirteen tests are presented and discussed.

Mycobacterium↗
PubMed1990

Experience of gas-liquid chromatography in clinical microbiology.

In clinical microbiology, gas-liquid chromatography (GLC) is used mainly for the following applications: (1) demonstration of microbial metabolites in cultures: studies of short-chain fatty acids (chain length 2 to 6 carbon atoms) and of the non-volatile lactic and succinic acids are the basis of taxonomy of obligate anaerobes on the genus level. Demonstration of these compounds in spent culture media is also used for identification of anaerobic bacteria in the clinical laboratory. (2) Investigation of long-chain fatty acids of cell membranes and the cell wall: analysis of fatty acids with chain lengths of 9 to 20 carbon atoms are used primarily for taxonomic purposes. A practical application for use in clinical bacteriology is the Microbial Identification System (MIS) based on equipment built by Hewlett-Packard. Although this system is excellently suited for quantitative analysis of long chain fatty acids and gives valuable preliminary or confirmative information, it often requires additional testing for identification of bacteria to species level. (3) Direct rapid diagnosis of infection: demonstration of metabolic products in body fluids can be of value in infections with anaerobes (demonstration of fatty acids) and yeasts (sugars). GLC combined with mass spectrometry or frequency-pulsed electron capture GLC techniques have been applied to studies of sera, fecal specimens, and cerebrospinal fluids. These latter applications are still in an experimental stage. However, the rapid diagnosis of tuberculosis directly from cerebrospinal fluid and sputum is well advanced. The potential of direct diagnosis without the resort to cultures warrants continued research with these techniques.

Bacteria, Anaerobic↗
PubMed1986

Impact of pregnancy termination on subsequent fertility.

The effects of pregnancy termination on subsequent fertility was studied in a group of 845 patients within a WHO organized multicentre trial. After comparing the index (abortion) and control groups by using the cumulative pregnancy rates per 100 women it was found at 30 months after recruitment to be 96.9 and 98.7, respectively. The difference was not significant statistically.

Abortion, Induced↗
PubMed1985

[The coincidence of gonadal dysgenesis and hyperprolactinemia].

It was reported about 6 female patients aged from 3 to 31 years (average age 18 years) with gonadal dysgenesis and hyperprolactinemia. Beside one all these females were not treated with synthetic sexual steroids. In 5 of the 6 patients both hyperprolactinemic and hypergonadotropic serum levels were observed. In one case the gonadotropic serum levels were normal. In 4 patients a stimulation test with GRH-TRH or Arg-GRH-TRH, respectively, was performed. In patients with gonadal dysgenesis a change on the area of the hypothalamic-hypophyseal axis is supposed to cause the hyperprolactinemia.

Adolescent↗
PubMed1985

[Primary hyperprolactinemic amenorrhea. Report of 4 patients].

Report about 4 female patients with hyperprolactinemic primary amenorrhea. In two of these women there was a suspicion of adenoma of hypophysis. In the stimulation test with gonadotropin-releasing hormone and thyrotropin-releasing hormone in two females the very high basal PRL secretion could not have been stimulated in one case. In the other case the TRH-stimulated PRL serum levels increased. Biphasic menstrual cycles resulted by therapy in two females treated with bromoergocryptine.

Adenoma↗
PubMed1984

Migration of I-cells from ectoderm to endoderm in Hydra attenuata Pall (Cnidaria, Hydrozoa) and their subsequent differentiation.

The cellular composition of isolated ecto- and endoderm of the gastric column of Hydra attenuata Pall were recorded qualitatively and quantitatively. The endoderm contains a small population of I-cells ("basal cells") which give rise to the endodermal neurons. The recombination of live ecto- and endoderm, one of which had previously been [3H]thymidine labeled, revealed that the endodermal I-cells and their neural derivatives originate from ectodermal I-cells which migrate across the mesoglea. No other cell types were found to pass from one cell layer to the other. The experiments support the idea that the endodermal gland cells constitute an autoreproductive cell line independent of the pluripotent I-cells.

Animals↗
PubMed1980

[Treatment of Asherman's syndrome (intra-uterine synechia) (author's transl)].

The authors gave treatment to 126 patients with Asherman's syndrome. The principles of that treatment are described, and the results are evaluated with regard to aetiology, localisation, and extent of adhesions. Menstruation was completely settled in 44.4 per cent of the above cases and partially in 19.8 per cent. Forty per cent of the sterile patients then turned pregnant. Twenty-two patients had normal deliveries and 20 miscarriages. Placenta praevia and accreta occurred quite often due to defective implantation. The therapeutic results depended primarily on the dimensions of the adhesions and on the functionality of the remaining endometrium.

Abortion, Habitual↗
PubMed1978

[Curettage during puerperium and its late consequences].

The authors examined data of 160 cases of puerperal curettage. The histological examination proved placental residue in 15,61 per cent, decidual residue in 65,61 per cent of the patients. It was a very important finding that the histological examination proved the presence of acute (66 patients), subacute and chronic (23 patients) inflammation, but the patients had no symptoms that would indicate endometritis. 71,86 per cent of the curettage were performed in the 2nd to 4th week of puerperium. Follow-up examinations were made in 69 patients in a period up to 1--5 years after the post-partal curettage. Out of the 69 patients only 14 were free of complications, 32 of them had amenorrhoe or hypomenorrhoe, 20 suffered from Asherman's syndrome (intrauterine synechiae).--49 of the patients intended delivery, but 22 were infertile. The authors' own principles on the treatment and prevention of puerperal hemorrhage are also summed up.

Curettage↗
PubMed1976

[Diagnosis of Asherman's syndrome (intrauterine synechiae)].

On the basis of 120 cases, the authors discuss the diagnostic problems of the Asherman's syndrome. They propose the accomplishment of hysterography in all cases when amenorrhea, hypomenorrhea, sterility or infertility develops after curettage during gestation period and puerperium. They describe the technical difficulties of X-ray examination, the appraisal of medicine certificates and the problems of differential diagnosis. The diagnosis of intrauterine synechia is usally based on the roentgen examination, but uterine sounding must also be performed, and these findings must be compared with the symptoms.

Abortion, Habitual↗
PubMed1975

[Management of female patients with the Stein-Leventhal syndrome].

The treatment by wedge resection of the ovaries in the Stein-Leventhal syndrome has been considered in 47 cases. The periods were restored to normal in 31 of cases. Ovulation occurred in 19 of cases. Pregnancy was achieved in 15 cases of 39 patients complaining of infertility. The authors discuss the method of wedge resection, the results of therapy, the problems of the recidivation and deal with the oncological, hormonal and psychical follow-up care of the patients. The authors' own principles of treatment are also summed up.

Female↗