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Biomedical subjects

I Schechter

Publications and source records attributed to I Schechter.

At least 109 records · Page 6Linked to original sources
PubMed1971

Prolonged survival of glutaraldehyde-treated skin homografts.

Treatment of mouse skin homografts in vitro with glutaraldehyde prolonged their average survival time from 12.4 to 39.2 days, presumably because the reagent became covalently bound to the histocompatibility antigen sites (or in their close vicinity) and shielded them from the immune apparatus of the recipient. The attachment to skin of the inert polymer poly((L)-lysine) via this bifunctional reagent increased the average survival time to 52.9 days. The simplicity and versatility of this approach might make it possible to screen a large number of reagents that can bind covalently to tissue constituents under physiological conditions. It seems possible that a particular treatment leading to a new contact surface in the transplant might favor the acceptance of homografts as well as of heterografts.

Alanine↗
PubMed1970

On the active site of elastase: Partial mapping by means of specific peptide substrates.

RNase-S peptide as well as some related octa- and hexapeptides were found to be highly, reactive substrates of porcine elastase (e.g. Ala(4)-Lys-Phe: K(m) = 4500 M(-1), k(cat) = 32 sec(-1), C = 1.4 x 10(5) M(-1) sec(-1)). Comparison of the various peptides led to the conclusion that the active site of porcine elastase is composed of 6-7 subsites (c.f. [1]). Preliminary mapping shows that subsites S(2), S'(1) and S'(2) have hydrophobic character. Occupation of subsite S(4) by the substrate is important for efficient hydrolysis. Binding at this subsite was found to be stereospecific.

Journal Article↗