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Biomedical subjects

I Sato

Publications and source records attributed to I Sato.

533 records · Page 30Linked to original sources
PubMedDate not supplied

Primary malignant lymphoma of the uterine corpus diagnosed by endometrial cytology. A case report.

BACKGROUND: A relatively small number of cases of primary malignant lymphoma of the uterine corpus have been reported, and it is rare for cases to be preoperatively diagnosed by cytology. CASE: A 59-year-old female experienced abnormal uterine bleeding of two months' duration. Preoperative evaluation of endometrial cytology revealed malignant cells. These cells demonstrated a rather round or oval configuration, with a markedly increased nuclear/cytoplasmic ratio, and were isolated and scattered in an inflammatory background. The nuclei were round or oval, and macronucleoli were marked. The cytologic diagnosis was malignant lymphoma. Postoperative histologic evaluation verified the presence of a primary malignant lymphoma in the uterine corpus, with a B-cell phenotype. CONCLUSION: Preoperative endometrial cytology correctly demonstrated malignant lymphoma of the uterine corpus.

Biopsy↗
PubMedDate not supplied

Appearance of primary endometrial and ovarian clear cell adenocarcinoma 17 months postpartum. A case report.

BACKGROUND: Rare cases of endometrial cancer have been reported within two years postpartum. However, clear cell adenocarcinoma of the endometrium does not appear to have been reported before as occurring in the early postpartum period. CASE: A 33-year-old-female experienced abnormal uterine bleeding 17 months after the delivery of her second child by cesarean section. Computed tomography indicated the presence of tumors in the uterus and cul-de-sac. Preoperative evaluation of endometrial cytology revealed adenocarcinoma cells. These cells demonstrated a centrally located nucleus and translucent, clear cytoplasm, with the so-called mirror ball pattern of cell clusters. The cytologic diagnosis was clear cell adenocarcinoma. Postoperative histologic evaluation verified the presence of a solid type of clear cell adenocarcinoma. The patient was considered to have simultaneous primary clear cell adenocarcinoma of the endometrium and ovary. CONCLUSION: Preoperative endometrial cytology correctly suggested the histologic type of cancer.

Adenocarcinoma, Clear Cell↗
PubMedDate not supplied

Apoptosis as a criterion for sensitivity of uterine cervical adenocarcinoma to cisplatin.

Two patients with uterine cervical adenocarcinoma received two courses of intra-arterial cisplatin administration. In the specimens of cervix 24 or 48 h after chemotherapy, marked apoptosis were observed. These bulky cervical tumors almost disappeared, and good PRs were confirmed by CT before operation. Apoptosis which is observed in the tissues of cervical adenocarcinoma 24 or 48 h after intra-arterial infusion of cisplatin, might be a new criterion of this chemotherapy effect.

Adenocarcinoma↗
PubMedDate not supplied

Rapid death following papillary serous carcinoma of the endometrium without myometrial invasion.

A 72-year-old woman was diagnosed with a stage I endometrial carcinoma, and total hysterectomy, bilateral salpingo-oophorectomy, and systematic pelvic and paraaortic lymphadenectomy were performed. Postoperative pathological examination determined that the tumor was confined to the endometrium, with no myometrial invasion or lymph-vascular involvement; histological examination revealed a papillary serous carcinoma of the endometrium. Peritoneal washing cytology during surgery revealed an adenocarcinoma. Despite postoperative adjuvant chemotherapy, early recurrence resulted in death 13 months after surgery. In the absence of myometrial invasion and lymph-vascular involvement, the data suggest that peritoneal washing cytology may serve as a prognostic factor in papillary serous carcinoma of the endometrium.

Aged↗
PubMedDate not supplied

An in vitro examination of a 5-fluorouracil regimen involving continuous venous infusion using cultured cell lines derived from ovarian cancers.

To examine the antitumor effectiveness of continuous venous infusion of 5-fluorouracil (5-FU), such a regimen was mimicked in vitro using cultured ovarian cancer cell lines. Two ovarian carcinoma cell lines, HRA and KK with cell doubling times (DT) of 16 h and 45 h, respectively, were grown under three incubation conditions with respect to 5-FU exposure, and the extent of growth inhibition was compared among the three conditions and between the two cell lines. Protocol I: 6 h-intermittent exposure to 5-FU, protocol II: 24 h-intermittent exposure to 5-FU, protocol III: uninterrupted exposure to 5-FU. The 50% growth inhibitory concentrations (IC50) of 5-FU obtained for KK cells grown under protocols I and II were both two times higher than that obtained under protocol III. Regarding HRA cells, IC50 obtained under protocol I was two times higher than that obtained under protocol III, while that obtained under protocol II was 3.7 times higher than that obtained under protocol III. Results obtained from the present in vitro examination suggested that the drug-free interval in the intermittent infusion of 5-FU should be shorter than the DT of the targeted tumor cells to obtain an efficacy corresponding to that obtained by uninterrupted exposure to the drug. In establishing an effective intermittent continuous infusion therapy with 5-FU with fewer side effects, the drug-free interval should be shorter than the DT of tumor cells, but longer than the DT of proliferating cells in normal tissues, including gastrointestinal mucosa cells assuming that these normal cells possess shorter DT than tumor cells.

Cell Division↗
PubMedDate not supplied

Origin of an 84-kDa protein with ABH blood-typing antigen activity in human seminal plasma.

In order to investigate the origin of an 84-kDa protein with ABH blood-typing antigen activity (p 84) and its concentration in human seminal plasma, a monoclonal antibody (mAb p 84) was produced. The protein was recognized in breast milk as well as in seminal plasma by an indirect, enzyme-linked immunosorbent assay (ELISA) using this mAb. mAb p 84 identified 84-kDa and 83-kDa forms of the protein in seminal plasma and breast milk, respectively, on immunoblotting. The mean concentration of p 84 in seminal plasma was 949 microg/ml (n = 54 subjects). There was no significant difference in the concentration of p 84 between individuals who secreted (Se) or did not secrete (non-se) the ABH antigen into their seminal plasma, nor were there any significant correlations between the concentration of p 84 and the total seminal protein concentration. An immunohistochemical study using mAb p 84 with light microscopic detection showed that p 84 was located in the cytoplasm of the inner layer of pseudostratified cuboidal epithelial cells of the seminal vesicles, but no immunoreactivity was found in the prostate. These data indicate that p 84 originates from a single tissue, the seminal vesicles, and suggest that p 84 is an ABH epitope-bearing protein that has not previously been identified but possesses some immunological properties similar to those of lactotransferrin.

ABO Blood-Group System↗
PubMedDate not supplied

Mutational analysis of transforming growth factor beta receptor type II and DNA mismatch repair genes in sporadic endometrial carcinomas with microsatellite instability.

To clarify how microsatellite instability (MI) is involved in carcinogenesis of sporadic endometrial carcinoma, we examined mutations of the transforming growth factor beta receptor type II (TGF beta RII) gene in 32 patients with MI-positive sporadic endometrial carcinoma. Moreover, mutations of 4 DNA mismatch repair (MMR) genes (hPMS1, hPMS2, hMLH1, hMSH2), which are considered to cause MI, were investigated as well. With respect to the TGF beta RII gene, mutations in the 10-bp polyadenine repeat sequence were observed in 7 of 29 informative cases (24%). Concerning MMR genes, a T to C point mutation at the -6 intronic splice acceptor site of exon 13 of hMSH2 was detected in 43% (6/14). However, there was no mutation in any exon of these 4 MMR genes. These results suggest that there is a carcinogenic mechanism via mutation of the TGF beta RII gene in some cases of MI-positive sporadic endometrial carcinoma. It seems unlikely that the unknown MMR genes are responsible for MI. The implication of the mutation at the intronic splice acceptor site in hMSH2 remains to be clarified.

Adaptor Proteins, Signal Transducing↗
PubMedDate not supplied

Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis.

To investigate the involvement of S. anginosus infection in head and neck cancer in the extra-oropharyngeal cavity, we analyzed 3 DNA samples prepared from squamous cell carcinoma of the external auditory canal and one from squamous cell carcinoma of the skin using polymerase chain reaction (PCR) analysis and Southern blot analysis to detect the DNA sequence of S. anginosus. We also examined these four specimens by Gram's stain to detect the streptococcal bacterial bodies. By PCR analysis, the DNA sequence of S. anginosus was found in 4 out of 4 (100%) DNA samples obtained from these tumors. By Southern blot analysis, positive bands were detected in one out of the 3 (33%) samples from the tumor taken from the external auditory canal. We detected streptococcal bacterial bodies in one of the three specimens from the tumor obtained from cancer of the external auditory canal and in the one specimen from the skin cancer by the method of Gram's stain. Contrary to our expectations, these bacterial bodies were located in the middle of the tumor. Since S. anginosus is thought to exist in the mouth as a normal flora and to be located mainly in the gingiva and dental plaque, these data strongly indicate that S. anginosus infection is implicated in the carcinogenesis of head and neck squamous cell carcinoma.

Aged↗
PubMedDate not supplied

Idiopathic hyperamylasemia.

A 25-year-old pregnant Japanese woman was diagnosed with idiopathic hyperamylasemia. The administration of a beta-stimulant caused a further increase in the serum levels of pancreas-type amylase and lipase.The patient's hyperamylasemia concomitant with elevated serum levels of lipase, elastase 1, and trypsin lasted over a 4 year-period, in which the patient remained asymptomatic. It is possible that the pancreas in some women with the idiopathic hyperamylasemia can respond to a beta-adrenoceptor stimulation with respect to pancreatic enzyme secretion.

Adult↗