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Biomedical subjects

I Sarov

Publications and source records attributed to I Sarov.

At least 109 records · Page 6Linked to original sources
PubMed1983

Detection and persistence of specific IgA antibodies in serum of patients with hepatitis A by capture radioimmunoassay.

The serum immunoglobulin A (IgA) response to hepatitis A virus (HAV) was investigated with a sensitive capture radioimmunoassay. In serial serum samples drawn from 15 patients with viral hepatitis A, IgA anti-HAV antibodies reached their highest titer between 1-2 weeks after onset and peak titers ranged from 10,000-20,000. Serum samples were available from six patients 30-32 months after onset of illness. These samples were all positive for IgA anti-HAV and some had titers similar to peak titers during illness. However, the height of the titration curves, expressed as the binding ratio (BR) at a dilution of 1/1000, was in all cases significantly lower at 30-32 months than during acute illness and early convalescence. The significance of the persistence of the IgA anti-HAV and possible reasons for the change in the BR are discussed.

Antibodies, Viral↗
PubMed1983

Specific IgG and IgA antibodies to herpes simplex virus and varicella zoster virus in acute peripheral facial palsy patients.

The role of herpes simplex virus (HSV) and varicella zoster virus (VZV) in acute peripheral facial palsy (APFP) was evaluated in 153 patients. The sera of patients were examined for IgG and IgA antibodies to HSV and VZV by the immunoperoxidase antibody-membrane antigen (IPAMA) technique. The prevalence of IgG and IgA seropositivity to HSV was significantly higher in the APFP patients than in the matched control group. No significant difference was found in the geometric mean titer (GMT) to HSV antibodies in the APFP patients compared to the matched control groups. The prevalence of IgA antibodies to VZV was significantly higher in the APFP patients group than in the matched control group. The GMT for VZV IgG antibodies was significantly higher in the APFP patients than in the matched control group. No significant difference was found in the GMT of VZV IgA antibodies. Eleven of 83 patients for whom paired sera were available had significantly increased or decreased IgG and IgA antibody titers to VZV on subsequent examination. Four of these patients did not show any evidence of zosterian eruption. These studies support the concept that VZV and HSV might have a role in the etiopathogenesis of APFP in some of the patients.

Acute Disease↗
PubMed1983

Human endometrium in cell culture: a new method for culturing human endometrium as separate epithelial and stromal components.

The present study describes a simple method for culturing human endometrium as separate epithelial and stromal components. Fifty-two samples of normal human endometrium have been initiated in tissue culture: endometrium from both the proliferative and luteal phases of the menstrual cycle showed satisfactory growth in vitro with a success rate of about 94%. Epithelial cultures remained viable for 60 days, while from stromal cells it was possible to establish cell lines. Both cell types possessed estrogen receptors. Epithelial cells showed no clear estrogen or progesterone response. Our observations suggest that this simple method for culturing human endometrium may serve as a tool in further investigations.

Connective Tissue↗
PubMed1983

Human cytomegalovirus specific IgA antibodies detected by immunoperoxidase assay in serum of patients with cytomegalovirus infections.

Cytomegalovirus (CMV)-specific IgA antibodies were determined by an immunoperoxidase assay in sequential serum samples of 10 patients with CMV infection in order to evaluate the feasibility of the use of this technique for diagnosis. In parallel, IgM and IgG antibodies to CMV were studied by enzyme-linked immunosorbent assay (ELISA) and by the immunoperoxidase assay, respectively. CMV IgA antibodies were detected in all 10 CMV patients studied. Specific IgM was detected earlier than IgA in only one of these ten patients. No CMV-specific IgA antibodies (titer less than 2) were detected in 45 medical students. Neither were they found in paired sera of 5 patients with herpes simplex infection, 5 patients with varicella, 6 patients with zoster and 2 patients with Epstein-Barr virus infection. The potential application of the indirect immunoperoxidase IgA assay for serodiagnosis of CMV infections is discussed.

Antibodies, Viral↗
PubMed1983

A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

A technique, using indirect immunoperoxidase antibody (IPA), was developed for the detection of IgG antibody to Chlamydia trachomatis. The IPA technique employs glass slides with air-dried and acetone-fixed C trachomatis infected cells, which can be stored at -70 degrees C and used for several months. Antibody titres detected by IPA were comparable to those detected by the indirect fluorescent antibody technique.

Antibodies, Bacterial↗
PubMed1983

Detection of varicella-zoster virus-specific IgA antibodies in varicella and zoster patients and in healthy adults of various ages by solid-phase radioimmunoassay.

The varicella-zoster virus(VZV)-specific serum immunoglobulin A(IgA) response was studied in varicella and zoster patients and in healthy adults of various ages by a sensitive solid-phase radioimmunoassay technique. Significant rises in VZV-specific IgA titer were found for both varicella and zoster patients, while VZV-specific IgA was also detected in greater than 90% of healthy adults but at lower titers. No significant decrease in VZV-specific IgA titer with increasing age was found. The role of humoral versus cell-mediated immunity in VZV reactivation is discussed.

Adult↗
PubMed1983

Rickettsial antibody prevalence in southern Israel: IgG antibodies to Coxiella burnetii, Rickettsia typhi, and spotted fever group rickettsiae among urban- and rural-dwelling and Bedouin women.

A retrospective serological survey was carried out using sera obtained from women at childbirth in the southern desert region of Israel to determine exposure experience to three rickettsial agents: Coxiella burnetii, Rickettsia typhi, and spotted fever group rickettsiae. Using the indirect fluorescent antibody method for determining IgG antibodies, it was found that about 40% of all sera examined demonstrated antibodies to one or more rickettsiae. Bedouin women appeared to be at greater risk of having antibodies to C. burnetii and spotted fever group rickettsiae than did Jewish residents of Beersheba, agricultural settlements, and development towns. The residents of development towns appeared to be at lower risk of developing antibodies to spotted fever group rickettsiae than did other populations sampled. Possible reasons for these differences are discussed.

Antibodies, Bacterial↗
PubMed1983

Early acquisition of cytomegalovirus infection in kibbutz children in comparison with urban and Bedouin children in southern Israel.

Prevalence of antibody to human cytomegalovirus (CMV) was determined in healthy children aged 1 to 13 years in Beer Sheva, in the Negev region in Israel. Kibbutz children showed a significantly higher rate of CMV seropositivity by the second year of life than did urban Jewish and Bedouin children. Kibbutz children live in close contact from the sixth week of life under high standards of hygiene and good socioeconomic conditions. Among children living in urban populations, as significantly higher prevalence of CMV seropositivity by the second year of life was associated with crowding, but not with other socioeconomic indicators (place of residence, country of origin, educational level of parents). A marked rise of CMV seropositivity with age was found in urban Jewish children in the 2- to 5-year age-group, during which time they attend nurseries, and in Bedouin children in the 6- to 9-year age-group, when they first attend school. These data suggest that close contact is of major importance in CMV infection in childhood.

Adolescent↗
PubMed1983

HSV-specific serum/CSF antibody ratio in association with HSV serum IgM antibodies in diagnosis of herpes encephalitis in infants.

Herpes simplex virus encephalitis (HSVE) has a reported mortality of 60 to 80%. Because of the recently published data on experience with the antiviral agent vidarabine, early diagnosis of this infection becomes imperative if enhanced therapeutic efficacy is to be achieved. At the present time the only way to diagnose HSVE with certainty is by brain biopsy. However, in cases with biopsy-proven HSVE, it has been shown that a rise in serum and cerebrospinal fluid (CSF) antibody titers to HSV, resulting in serum: CSF ratios of less than 20, may be diagnostic for HSVE. Three infants, aged 5, 7 and 12 months, are described. All of them presented with fever, convulsions and CSF pleocytosis which, together with computer tomography scan findings, were suggestive of meningoencephalitis. The diagnoses of HSVE were made by IPAMA (immunoperoxidase antibody to membrane antigen) for determination of HSV-specific IgG titers, and ELISA (enzyme-linked immunosorbent assay) for determination of HSV-specific IgM titers. All three infants had HSV-specific IgM in their serum, as well as HSV-specific serum IgG:CSF IgG ratios of less than 20. Two infants were treated with vidarabine with significant improvement in one and complete recovery in the other. Increased clinical awareness of HSVE symptoms, combined with the application of these rapid methods of diagnosis, might significantly improve the outcome of HSVE.

Antibodies, Viral↗
PubMed1983

Virus-specific secretory IgA antibodies as a means of rapid diagnosis of measles and mumps infection.

A highly sensitive solid-phase radioimmunoassay has been developed for the detection of virus-specific IgA antibodies in saliva obtained from infants and children. Serial saliva samples from infants and children under 3 years of age with acute measles or mumps infection, or after measles immunization, were examined for their content of virus-specific IgA antibodies. Virus-specific antibodies appeared transiently in the saliva in both illnesses and after immunization with live attenuated measles vaccine. The significance of this finding for rapid diagnosis of these infections without the necessity of drawing a blood sample is discussed.

Adolescent↗
PubMed1982

Detection of human cytomegalovirus- specific IgA antibodies in colostrum by enzyme-linked immunosorbent assay (ELISA).

Fifty women were examined after delivery for the prevalence of antibodies to human cytomegalovirus in colostrum and sera. Eighty percent of them had specific CMV IgG antibodies in the sera, as determined by the immunoperoxidase antibody to membrane antigen (IPAMA) technique. Of the CMV-seropositive women, 60% had specific CMV IgA antibodies in high titer in the colostrum as determined by enzyme-linked immunosorbent assay (ELISA). In only two of the seropositive women were specific CMV IgA antibodies detected in the sera as well. The significance of specific CMV IgA antibodies in colostrum as protection against perinatal infection and the mechanism of this production will be discussed.

Antibodies, Viral↗
PubMed1982

Prevalence of antibodies to human cytomegalovirus in urban, kibbutz, and Bedouin children in southern Israel.

Prevalence of antibody to human cytomegalovirus (CMV) was determined in a sample of 860 healthy children aged 1-13 years in Beer Sheva, in the Negev region of Israel. Three groups of children were tested: (1) urban Jewish children of low, middle, and high socioeconomic levels; (2) 8 rural communes (kibbutz) in which children live in close contact with each other from the 6th week of life under good hygienic conditions and a high standard of living; (3) Bedouin, seminomadic Arabs living in relatively poor hygienic conditions in the desert. Kibbutz children showed a significantly higher rate of CMV seropositivity by the second year of life than urban Jewish and Bedouin children (76% versus 44% and 54% respectively) with a gradual increase to 94% in the 10-13-year age group. Among children living in urban populations a significantly higher prevalence of CMV seropositivity was associated with crowding, but not with other socioeconomic indicators (place of residence, country of origin, or education level of parents). A marked rise of CMV seropositivity with age was found in urban Jewish children in the 2-5-year age group during which time they attend nurseries (44% to 67%) and in the Bedouin children in the 6-9-year age group (59% to 86%) when they first attend school. The data suggest that close contact is of major importance in CMV infection in childhood. The clinical implications of early acquisition and high prevalence of CMV antibodies in the kibbutz setting are discussed.

Adolescent↗
PubMed1982

Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies.

Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.

Antibodies, Viral↗
PubMed1982

Enzyme-linked immunosorbent assay (ELISA) for detection of specific IgA antibodies to mumps virus.

A sensitive enzyme-linked immunosorbent assay (ELISA) is described for detection of IgA antibodies to mumps virus. Specific mumps IgA antibodies could be demonstrated in 10 patients with mumps virus infections. No specific mumps IgA antibodies (titres <1/40) were detected by ELISA in 46 control sera (healthy adults; hospitalised patients with various other diseases). The potential application of the ELISA mumps IgA technique in serodiagnosis of mumps infections is discussed.

Adult↗
PubMed1982

Determination of specific IGA antibodies to varicella zoster virus by immunoperoxidase assay.

An indirect peroxidase technique was developed for determination of IgA antibodies to varicella zoster virus (VZV). The antigen consisted of acetone-fixed trypsinised VZV-infected cells. Rabbit antihuman IgA peroxidase conjugate was used to detect human IgA antibodies bound to viral antigen. In parallel IgG antibodies to VZV were determined by an immunoperoxidase antibody to membrane antigen (IPAMA) technique. Varicella zoster virus IgA antibodies were detected in all five varicella and seven zoster patients. No VZV IgA antibodies (less than 2) were detected in 45 healthy control sera. Neither were they found in paired sera of five patients with herpes simplex infection, five patients with human cytomegalovirus infection and two patients with Epstein-Barr virus infection. Application of immunoperoxidase IgA technique in serodiagnosis of primary and reactivated VZV infections is discussed.

Adult↗
PubMed1982

Detection of virus-specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzymeimmuno and radioimmunoassay techniques.

The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in eight renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-liked immunosorbent assay (ELISA) and immunoperoxidase assay (IPA) techniques were used for IgA antibody determinations. In parallel, IgG antibodies to CMV were studied by immunoperoxidase assay. A significant rise of CMV-specific IgG antibody titre was observed in all of these patients between 5 and 53 weeks post-transplantation. CMV-specific IgA antibody production was detected close to the time a rise in CMV IgG antibody was observed in seven out of eight patients studied by RIA and ELISA, and in six out of eight patients studied by IPA. In two patients specific CMV IgA antibodies were detected by all three methods before a significant rise of CMV IgG antibody titre was demonstrated. In these patients CMV IgA was detected by RIA earlier than by ELISA and IPA. The potential application of CMV-specific IgA antibody determination for early detection of recurrent CMV infection in renal transplant patients is discussed.

Adult↗