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Biomedical subjects

I Salazar

Publications and source records attributed to I Salazar.

At least 37 records · Page 2Linked to original sources
PubMed1992

Anatomical and immunohistological demonstration of the primary neural connections of the vomeronasal organ in the dog.

Macro- and microdissection methods together with conventional histology and lectin immunohistochemistry have been used to identify the course of the vomeronasal nerves and their site of termination (accessory olfactory bulb; AOB) in the dog. The AOB in this species is small and variable in size, situated on the medial surface of the main olfactory bulb, and has an anatomical structure unlike that described for other mammals. The vomeronasal nerves and their terminal glomeruli in the AOB are easily identifiable by selective immunohistochemical staining using Ulex europeus agglutinin I.

Animals↗
PubMed1991

Development of the mesonephros, metanephros and gonads in the foetal dog.

We describe the chronology of the development of the renal system and gonads of 19 greyhound foetuses. The smallest specimens were embedded in paraffin wax and were sectioned serially at 10 microns; the rest were sectioned at 50 microns in an LKB 2250 PMV cryomicrotome. Selected sections were stained with haematoxylin and eosin. The mesonephros, metanephros and gonads were measured in drawings of the sections, using the area fraction programme of a Vids II image analyse.

Animals↗
PubMed1991

Morphological and morphometric development of the stomach and intestine of the foetal dog.

We describe the chronology of development of the stomach and intestine in 18 greyhound foetuses. Using an LKB 2250 PMV cryomicrotome, we obtained corresponding series of sections (50 microns). Very small specimens were embedded in paraffin wax and sectioned at 10 microns. Selected sections were stained with haematoxylin and eosin. For measurement, the fractional area programme of a Vids II image analyser was employed.

Animals↗
PubMed1991

Growth curve of the foetal dog and the morphometry of its internal cavities and organs.

In the present study we determined the foetal growth curve of the greyhound. Using an LKB 2250 PMV cryomicrotome, we studied selected sagittal sections from 15 foetuses of the same breed of dog and described the expansion and development of the thoracic and abdominal cavities, the lungs, the liver and (in part) the heart and the thymus. Drawings of the sections were measured by means of a Vids II image analyser and its fractional area programme.

Animals↗
PubMed1991

Vascularization of the vomeronasal organ in the dog.

Blood is led to the vomeronasal organ via the large palatine and sphenopalatine arteries and returns via the veins of the same names. Under the microscope, transverse sections exhibit large numbers of veins and lymph vessels and relatively few arteries. The prominent veins are characterized by their thick, muscular wall; the arteries are smaller in proportion and likewise muscular, while the typical lymph vessels are dilated in sections of organ prepared by injection of latex or Araldite. Due to the presence of erectile tissue, the arrangement of the parenchyma depends on the functional state of the organ.

Animals↗
PubMed1990

Glucagon-like immunoreactivity in hypothalamic neurons of the rat.

Antisera specific for three different regions of pancreatic proglucagon were used to examine the distribution of such immunoreactivity in rat hypothalamus. Neurons in the supraoptic and paraventricular nuclei were immunoreactive with an antiserum against glucagon, but not with antisera directed towards the aminoterminal region of proglucagon (glicentin) or the glucagon-like peptide I sequence in the carboxyl-terminal region of proglucagon. These findings confirm a previous report of glucagon-like immunoreactivity in the supraoptic and paraventricular nuclei, but indicate that, while this material is immunochemically related to glucagon, it is not derived from a proglucagon-like precursor.

Animals↗
PubMed1989

The thalamus of the dog: a tridimensional and cytoarchitectonic study.

The organization of the thalamus varies considerably from species to species. This article reports a tridimensional study of the canine thalamus in which 18 nuclei were distinguished on the basis of their delimitation in sections and their differing cytoarchitectonic characteristics, the latter having been objectively quantified by means of an image analyser. On the basis of their location and/or mutual similarities, these 18 nuclei are classified in 5 groups. The thalamus of the dog is similar to that the cat, but differs considerably from those of other well-studied species.

Animals↗
PubMed1988

Topography and microscopical configuration of the corpus striatum of the dog.

The basal ganglia are very similarly disposed in superior animal, but their way of grouping is different in human species and domestic mammals. We have studied them in the dog, except for the Nucleus accumbens, from macroscopic and microscopic points of view, with the help of 25 and 50 pm thick transverse serial sections, stained by Nissl's technique. Each of these nuclei presents its own characteristics, as well topographically and extended as of shape, size and neuron density.

Animals↗
PubMed1985

Effect of benzo[a]pyrene on DNA synthesis and DNA polymerase activity of rat liver nuclei.

When benzo[a]pyrene (B[a]P) was administered intraperitoneally to rats 48 hr before they were killed, the DNA-synthesizing capability of isolated rat liver nuclei was decreased as compared with control animals. B[a]P also inhibited in vitro DNA synthesis in nuclei purified from control animals; this effect was enhanced by NADPH. DNA polymerases solubilized from purified nuclei of B[a]P-treated animals were less active than those of control animals. DNA polymerase alpha was more inhibited than DNA polymerase beta. Purified rat liver nuclei devoid of cytoplasmic contamination possess an NADPH-dependent B[a]P hydroxylase activity. The observed inhibition of DNA synthesis in nuclei isolated from B[a]P-treated rats was increased by NADPH. Moreover, there was an increased inhibition of DNA polymerase activity by nuclear membranes obtained from B[a]P-treated animals when the incubations were performed in the presence of NADPH. Also, the derivative B[a]P-trans-9,10-dihydrodiol was a potent inhibitor of DNA polymerase alpha under conditions where DNA polymerase beta was less affected. These results suggest that nuclear B[a]P hydroxylase might be involved in the inhibition of DNA synthesis probably at the level of DNA polymerase alpha. As in the in vivo studies, the nuclear polymerase most affected by the hydrocarbon in vitro was DNA polymerase alpha.

Animals↗
PubMed1984

Spavin: a proposed term for a non-fracture associated canine hock lesion.

In accordance with macroscopic and microscopic features of the tarsal joint degeneration in the greyhound dog, the possibility of establishing a direct relation between this process and spavin or bone spavin is proposed. To date this has been considered limited to equine and occasionally bovine stock. This investigation has been carried out on three different levels: macroscopic, radiological and histological and has been completed with the corresponding graphic documentation.

Animals↗
PubMed1984

The relation of the lymphatic system to hemolymph nodes in the sheep.

Sublumbar lymphatic drainage was examined in fetal and adult sheep for potential connections with hemolymph (hemal) nodes. Despite numerous channels entering sublumbar lymph nodes there were no lymphatic communications with hemal nodes. In ovine ruminants hemolymph nodes are separate from the lymphatic system.

Animals↗