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Biomedical subjects

I Ríha

Publications and source records attributed to I Ríha.

32 records · Page 2Linked to original sources
PubMed1981

Regulation of antibody response to SRBC. I. Influence of corpuscular and soluble form of erythrocyte antigen and antigen dose on antibody response in A/J and C57BL/10ScSn mice.

Mice of the inbred strain C57BL/10ScSn compared to the A/J strain produce ten times fewer IgG plaque-forming cells and serum IgG antibodies after primary and secondary SRBC immunization. Even after a repeated immunization with SRBC, the titres of IgG anti SRBC antibodies in serum do not reach the levels found in A/J mice. On the other hand, IgM anti SRBC antibodies are higher in C57BL/10ScSn mice and remain high, even after repeated immunization. Doses of antigen ranging from 1 X 10(7) to 1 X 10(10) SRBC per mouse i.p. did not change significantly the response of C57BL/10ScSn mice. Only the highest dose slightly increased IgG anti SRBC antibody production. The use of a soluble erythrocyte antigen with similar antigenic properties as the original corpuscular form did not change the nature of IgM and IgG anti SRBC antibody response.

Animals↗
PubMed1981

Regulation of antibody response to SRBC. II. Effect of a non-specific stimulation.

The ratio of individual 78 isotypes after primary and secondary immunization with SRBC was studied in the strain with a normal formation of IgG antibodies (A/J) and in the strain which responds predominantly by formation of IgM antibodies and only by a low IgG antibody response (C57BL/10ScSn, hereafter referred to as B10). The greatest differences were found in the amount of PFC which produce the IgG2a isotype. In B10 mice the IgG2a/IgM ratio is more than 100 times lower than in A/J mice. The so-called "natural antibodies" to SRBC were detected in both strains and were only of the IgM class; they were slightly higher in B10 mice. Using haemagglutination inhibition test we did not find greater differences between SRBC and erythrocytes of B10 mice than between SRBC and erythrocytes of A/J mice which might explain the lower levels of IgG antibodies after immunization with SRBC. T lymphocytes play an important role for the IgM-IgG switch. This activity of T lymphocytes can be partially substituted by certain substances like LPS or even CFA. We attempted therefore to influence the level of IgG in B10 mice by administration of 10 micrograms of LPS i.p. simultaneously with SRBC. In the high responder strain A/J the administration of LPS led predominantly to an increase in IgG-producing PFC, while the amount of IgM increased only slightly. The effect of LPS on B10 mice was somewhat different. It increased the level of IgG to such an extent that it was comparable to the level seen in A/J mice; however, it also increased the number of IgM-producing PFC so that the final ratio of IgG/IgM remained low in B10 mice.

Animals↗
PubMed1978

Simple method of circulating immune complex detection in human sera by polyethylene glycol precipitation.

A rapid test for detection of circulating immune complexes in a small serum sample was developed to facilitate clinical diagnosis of immune complex disorders. The test is based on a selective precipitation of soluble circulating complexes of antigen-antibody in 3.75% concentration of high-molecular polyethylene glycol. Precipitation is followed photometrically at 450 nm, 1 cm cuv. after 1 h incubation at room temperature. Comparison of E450 values in groups of patients with immune complex disorders, such as rheumatoid arthritis, systemic lupus erythematosus and glomerulonephritis, with healthy controls or patients with non-immunological disorders revealed highly significant differences. Sera of all patients with high clinical activity of disease exhibited positive reaction. In 121 human sera the results of this examination were compared with the results of C 1 q binding test. There was 73.5% agreement between the results of both methods. Our test is more rapid, suited for routine clinical use.

Antigen-Antibody Complex↗
PubMed1975

Experimental immune complex glomerulopathy: immunomorphological aspects and correlations with human disease.

Thirteen rabbits received parenteral injections of human serum albumin (HSA) in regular doses and intervals during 6-32 seeks. The antigen load elicited immune responses of variable degree, giving a good correlation with the extent and quality of immune complex (IMC) deposition, as shown by immunological methods, electron and fluorescence microscopy and by histoautoradiography. Morphology of immune deposits (IMD) depended on the composition of the IMC and produced a continuous scale of glomerular involvement, ranging from minimal and focal to massive and diffuse deposition. Probable rudimentary traces of IMD were apparent in the ultrastructure even in animals with negative results of both immunological and immunfluorescence examinations. The relationships are discussed between experimental IMC-induced glomerulopathy on the one hand, and some problems of development and bioptic evaluation of human glomerulonephritis.

Animals↗