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Biomedical subjects

I P Chernov

Publications and source records attributed to I P Chernov.

At least 19 recordsLinked to original sources
PubMed1999

[Identification and mapping on human chromosome 19 of chromosome-specific repeated element preferentially binding with nuclear matrix].

From a library of sequences binding preferentially to nuclear matrix (matrix attachment regions, MARs), a fragment of about 300 bp in length (CEA (carcinoembryonic antigen)-MAR) was isolated and characterized. The CEA-MAR sequence was found in more than ten loci of chromosome 19 containing elements similar to genes of the CEA family. No sequences of this group were found on other human chromosomes. Two CEA-MAR-containing loci were sequenced, and sequences for another seven loci were found in GenBank. A comparative analysis of CEA-MARs and the flanking sequences is reported. Based on the sequence of the CEA-containing chromosome 19 loci, a hypothetical model of the domain structure of a 2-Mb chromosome region was constructed and the mutual arrangement of CEA-MARs and genes of CEA family was elucidated. The CEA-MARs were located 5-20 kb downstream of the CEA genes. These results suggest that the duplication unit of the CEA family may coincide with chromatin domains containing these genes.

Base Sequence↗
PubMed1993

High-performance liquid chromatography of the photoproducts of nucleic acid components. III. Detection of the secondary structure differences in sequence isomeric self-complementary oligonucleotides.

Reversed-phase high-performance liquid chromatography (RP-HPLC) was used to detect differences in the secondary structures of two self-complementary oligodeoxyribonucleotides. The [d(CCTTTAAAGG)]2 duplex assumes an ordinary B-conformation in aqueous solution, while [d(GGAAATTTCC)]2 is known to contain in its central part a stretch of a more rigid B'-form conformation with significantly lowered fluctuational mobility of base pairs. The latter factor causes a marked difference in the amounts of thymine cyclobutane photodimers formed under UV irradiation of corresponding duplexes as revealed by chromatography of two single-stranded oligonucleotides. Increasing the temperature below the duplex melting temperature (stimulation of the B'-B structural transition) results in an increase in photodimer formation that was inhibited in the B'-form. Thus, we demonstrate the usefulness of RP-HPLC for duplex DNA structural studies.

Base Sequence↗
PubMed1991

[Isolation of recombinant interleukin-3 produced by E. coli].

A synthetic gene coding for human interleukin-3 (hIL3) was cloned in the plasmid pTE2IL3, the gene expression being controlled by the phage fd PVIII promotor and the phage T7 gene 10 translational enhancer. Under constitutive biosynthesis conditions in E. coli, the accumulation of recombinant hIL3 (in the inclusion bodies) was up to 30-40% of the total cell protein. An effective procedure of the hIL3 isolation is suggested. The hIL3 was solubilized in 5 M guanidinium chloride, renaturated and purified to homogeneity by a single chromatographic step. The protein's yield was 34 mg/g wet cells. The isolated hIL3 showed a specific biological activity.

Chromatography, Gel↗
PubMed1989

Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny.

In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Ag1 (in a loop located between two beta-strands in poly-peptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a 'nonsense' and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be 'transplanted'.

Amino Acid Sequence↗
PubMed1989

Effective method for obtaining long nucleotide chains on partially complementary templates. Processed bovine gamma-interferon gene obtained from human gamma-interferon gene.

The method of obtaining the bovine gamma-interferon gene by means of simultaneous multidirected mutagenesis of the human gamma-interferon gene is presented. The first strand of the bovine gamma-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human gamma-interferon, cloned in the single-stranded phage M13mp19 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase I. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC-18 derived vector. The primary structure was confirmed by sequencing.

Animals↗
PubMed1989

[Isolation of the porcine alpha-interferon gene using simultaneous directed multipoint mutagenesis].

A method of obtaining the pig alpha-interferon gene by means of simultaneous multidirected mutagenesis of the human alpha 2-interferon gene is presented. Nucleotide homology between these genes is 80.4%. Fourteen synthetic oligonucleotides forming a pig alpha-interferon gene's strand were ligated on a single-stranded template, carrying cDNA of the human alpha 2-interferon gene. The obtained DNA fragment was cloned in the single-stranded or double-stranded form. It was found that the method does not affect the cloning efficiency. The primary structure of the gene was confirmed by sequencing.

Amino Acid Sequence↗
PubMed1987

trp operon induction during the expression in E. coli of two IFN-gamma sequences.

Two nucleotide sequences coding for mature human immune interferon (IFN-gamma) and differing from each other by nine N-terminal nucleotides were expressed in E. coli under the control of a trp promoter. The longer gene variant after the ATG initiatory codon contained a TGT TAC TGC sequence, which was absent in the shorter gene. When expressed in E. coli under the direction of identical transcription and translation regulatory elements, these genes showed different susceptibility to induction.

Base Sequence↗
PubMedDate not supplied

[Structure and function of nuclear matrix associated regions (S/MARs)].

Modern concepts on the chromatin loop-domain organization and the role of the DNA regions specifically binding the nuclear matrix (nuclear scaffold, or S/MARs) in its formation, maintenance, and regulation are discussed. Some S/MAR structural features, properties of binding the nuclear matrix, and probable mechanisms of their involvement in regulation of gene activity are considered. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru.

Amino Acid Sequence↗
PubMedDate not supplied

[Histophysiology and histopathology of the adrenals in experimental hypokinesia].

In experiments on male rats in the course of 3-month hypokinesia phasic changes were observed in the relative adrenal gland weight, in the volume of the cell nuclei of the glomerular, fasicular zones and the medulla, in the activity of the succinic dehydrogenase, alkaline and acid phosphatases, in the RNA and catecholamine content. These changes are associated with variations in the secretion and biosynthesis of the hormones of the cortical and the medullary layer of the adrenal glands during the hypokinetic stress development.

Adrenal Cortex↗
PubMedDate not supplied

[Effect of agents used in the therapy of acute ischemic heart disease on the histomorphological changes in the myocardium in acute dystrophy].

The morphological analysis of the portions of the interventricular septum and left ventricular wall in cats has revealed dystrophic and necrotic changes in the myocardium after administration of caffeine followed by adrenaline: haemorrhages, intravascular thrombogenesis, dystrophic and necrobiotic changes in myocytes. In early periods of acute ischemic heart disease morphine, fibrinolysin, heparin and Inosie F either abolished (fibrinolysin) or considerably decreased (heparin and Inosie F) a tendency toward intravascular thrombogenesis and damage to the myocardial parenchyma.

Acute Disease↗
PubMedDate not supplied

[Bioelectrical, biochemical and histomorphological changes in the ocular tissues in pituitrin hypertension and the use of euphylline and papaverine].

Experimental study on rabbits of bioelectric and biochemical characteristics of eye tissues during pituitrin hypertension and application of euphylline and papaverine as well as comparison of the data obtained indicate that during pituitrin hypertension, transtissue potential difference and density of short-circuit current in the lens are considerably increased whereas the level of tissue respiration in the eye is reduced. Administration of euphylline or papaverine during pituitrin hypertension aggravates hemodynamic disorders in eye tissues, deteriorates the bioelectric and biochemical characteristics in both hypertensive and control animals.

Aminophylline↗