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Biomedical subjects

I Olsson

Publications and source records attributed to I Olsson.

At least 199 records · Page 11Linked to original sources
PubMed1975

Cationic proteins of human granulocytes. VI. Effects on the complement system and mediation of chemotactic activity.

The chymotrypsin-like cationic proteins of human granulocytes are shown to possess the ability to produce conversion of the complement components C1s, C4, C3, and C5 as detected by crossed immuno-electrophoresis. This ability seems to be a direct proteolytic effect. Incubation of cationic proteins with serum or functionally pure preparations of C3 and C5 is shown to generate the formation of chemotactic activity which is abolished by prolonged incubation. Also, the chemotactic activity of porcine C5a or spontaneously activated C5 is abolished by incubation with cationic proteins. It is suggested that the chymotrypsin-like cationic proteins of human granulocytes after extrusion from the phagocytic cell play an important role for generation of inflammatory mediators.

Antigen-Antibody Reactions↗
PubMed1975

Cationic proteins of human granulocytes. IV. Esterase activity.

The purfied antibacterial cationic proteins 1 to 7 (component 1 being the most cationic) of human neutrophil granulocytes have been assayed for enzyme activities. Components 1 to 4 and component 7 exhibited activities on tyrosine esters indicating a chymotrypsin-like activity with a neutral pH optimum. The inhibition of the esterase activity by diisopropyl fluorophosphate and phenylmethyl sulfonylfuoride also indicated the similarity to chymotrypsin, with a serine residue in the active center. In addition, the cationic proteins attacked complex substrates like casein and fibrinogen, suggesting a true proteolytic activity. Since the esterase activity of these proteins is heat-labile but the antibacterial activity against Staphylococcus aureus is heat-stable, it is concluded that the chymotrypsin-like activity is not essential for the microbicidal activity. There is evidence to suggest that the cationic proteins of human granulocytes are present in the cytoplasmic granules and exhibit antibacterial activity after release to the phagocytic vacuole and that, after extrusion from the cell, the proteins might be involved in the mediation of the inflammatory process due to enzyme and cationic activities.

Arginine↗