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Biomedical subjects

I Okazaki

Publications and source records attributed to I Okazaki.

At least 55 records · Page 3Linked to original sources
PubMed1992

[Measurement of serum type III procollagen aminopeptide with RIA-gnost PIIIP coated tube: changes in patients with various alcoholic liver diseases after abstinence].

In 55 patients with various alcoholic liver diseases, serum type III procollagen aminopeptide (PIIIP) was measured on admission, on biopsy (2-4 weeks after admission) and on discharge from hospital (3 months after admission) using RIA-gnost PIIIP Coated Tube, an assay kit recently launched by Hoechst Japan Limited. Serum PIIIP on admission showed a good correlation with the degree of hepatic fibrosis and was significantly higher in any disease group than in healthy controls, suggesting its usefulness as an indicator of hepatic fibrosis. After abstinence, however, serum PIIIP level exhibited an increasing tendency in all the disease groups except alcoholic hepatitis, and the number of laboratory tests showing a significant correlation with PIIIP decreased as compared with that on admission. The mechanism of serum PIIIP elevations seen after abstinence is yet to be elucidated in detail, considering their possible association with factors other than collagen synthesis in the liver.

Adult↗
PubMed1991

Formation and degradation of basement membrane collagen.

Serum levels of type IV collagen and hepatic type IV collagenase activity, as markers for formation and degradation respectively of basement membrane collagen (type IV collagen) in liver, were measured in patients with various alcoholic liver diseases. Development of alcoholic liver fibrosis seems to be due to both increased formation and decreased degradation of basement membrane collagen. Both measurements of type IV collagenase activity and serum level of type IV collagen were assumed to be useful to predict irreversible state of hepatic fibrosis.

Basement Membrane↗
PubMed1989

Prolylhydroxylase and collagenase activities in the healing of acetic acid-induced gastric ulcers in rats and the effects of cimetidine and elcatonin.

We examined the changes in prolylhydroxylase (PH) and interstitial collagenase (CA) activities in the healing of acetic acid-induced gastric ulcers in rats. Gastric ulcers were induced by subserosal injection of acetic acid, and confirmed by endoscopy on the third day. At the acute stage of ulceration, PH activity was not high but CA activity was increased. At the healing stage, PH activity was increased but CA activity gradually decreased to a normal level. Cimetidine did not effect PH activity but tended to increase CA activity in the healing. On the contrary, elcatonin increased PH activity but had little effect on CA activity. The above enzymes seems to be indispensable in the healing of gastric ulcers and effects of antiulcer agents on these enzymes must be considered.

Acetates↗
PubMed1989

Elevation of laminin and beta-subunit of prolyl 4-hydroxylase in the sera of human subjects with Schistosomiasis mansoni.

Serum levels of several markers for liver fibrosis were measured utilizing three groups of human subjects related with schistosomiasis mansoni in northeast Brazil; (1) 20 Schistosoma mansoni egg-positives, who have never been administered with anti-schistosomal drugs, (2) 29 egg-negative inhabitants in the endemic area of schistosomiasis, and (3) 23 egg-negative Japanese immigrants in the non-endemic area. None of these sera were positive for antibody to the surface antigen of human hepatitis B (HBs) and circulating HBs antigen. There was no significant difference in the serum levels of N-terminal peptide of procollagen type-III between the egg-positive subjects and either of the egg-negative Brazilian or Japanese immigrants, whereas the mean value of serum laminin significantly increased in the egg-positive subjects. A significantly higher concentration of serum immunoreactive beta-subunit of prolyl 4-hydroxylase (IR beta PH) was also observed in the egg-positive subjects only in comparison with that of the egg-negative Brazilian. Serum laminin and IR beta PH concentrations of the egg-positive subjects did not correlate with the absorbance of enzyme-linked immunosorbent assay (ELISA) which utilized crude antigens isolated from schistosome adults or eggs. No significant difference in these two parameters was observed between two subgroups of the egg-negative Brazilian or Japanese immigrants divided according to the serological data by ELISA. These findings suggest that serum laminin and IR beta PH levels are worth further evaluation for their usefulness as the marker for liver fibrosis in schistosomiasis.

Adolescent↗
PubMed1988

Assays of serum laminin and type III procollagen peptide for monitoring the clinical course of diabetic microangiopathy.

Thickening of the capillary basement membrane is a characteristic feature of diabetes and is considered to cause diabetic microangiopathy. Serum levels of both laminin, a glycoprotein in the basement membrane, and type III procollagen peptide (P-III-P) were measured by specific radioimmunoassays according to the methods of Brocks et al. and Rohde et al. respectively and analyzed with regard to diabetic microangiopathy, glycemic control, diabetic duration and treatment. As a result, serum levels of both laminin and P-III-P showed higher values with development of diabetic microangiopathy, suggesting that progressive changes in diabetic microangiopathy occur with synthesis of laminin and P-III-P. Serum laminin and P-III-P appear to be good non-invasive markers for measuring basement membrane metabolism and type III collagen accumulation. High values of serum laminin and P-III-P levels were suspected to be due to improper treatment, using hypoglycemic agents without adherence to the diet regimen.

Biomarkers↗
PubMed1987

Serum type III procollagen peptide: indicator for pulmonary fibrosis: II. Application in 80 asbestos insulation workers.

An assay for type III procollagen peptide (P-III-P) was applied to serum from 80 asbestos insulation workers in order to evaluate its relationship with pulmonary and pleural fibrosis. The serum peptide levels of 16 control workers were 7.8 +/- 2.0 ng/ml (mean +/- SD). An association between the serum values of P-III-P and the profusion of small irregular opacities on chest X-rays was noted. The levels in the group with moderate interstitial pulmonary fibrosis [profusion category 2(2/1, 2/2, and 2/3)] and with marked interstitial pulmonary fibrosis [profusion category 3(3/3)] were 9.9 +/- 2.3 ng/ml (n = 5) and thus tended to be higher than those of 16 control workers (p less than 0.1). On the other hand, there was not correlation between serum peptide levels and pleural lesions nor between the former and duration of asbestos exposure. From the results of the investigation [part I of which is reported elsewhere (Okazaki et al, 1986)] this assay may be useful for detecting individuals with a more marked tendency for progression of interstitial pulmonary fibrosis due to asbestos exposure in insulation work.

Adult↗
PubMed1986

[Serum type III procollagen peptide in diagnosis of lung fibrosis due to silicosis and bleomycin toxicity].

Early diagnosis of lung fibrosis has been hampered by the lack of a simple, convenient and specific test. Measurement of serum type III procollagen peptide (Pro (III)-N-P) by the method originally developed by Rohde et al. has been shown to be useful for the evaluation of hepatic fibrosis. The present study, therefore, was carried out to investigate the usefulness of the measurement of Pro (III)-N-P in 24 patients with lung fibrosis due to silicosis, and in 7 patients with malignant lymphoma treated with bleomycin, antitumor antibiotic which has the adverse effect of producing fibrosis in the lung. The normal value of the peptide in adults was 8.60 +/- 2.35 ng/ml (mean +/- SD; n = 68) and the normal upper level was set at 13.4 ng/ml (mean +/- 2SD). Patients with silicosis had significantly but not extremely high levels of the peptide and 25% of the patients showed abnormally high values. The level of Pro (III)-N-P was associated with neither physical findings, chest X-p findings nor pulmonary function test results. Three of 7 patients showed increased levels during treatment with bleomycin. In one case, a total dose of 120 mg of bleomycin for over a period of 14 months markedly increased the level of the peptide. These observations suggest that the determination of Pro (III)-N-P may be useful for the detection of lung fibrosis.

Adult↗
PubMed1986

[Epidemiological study on alcohol consumption and physical health in the Kitakyushu area with special reference to industrial structure].

Kitakyushu city is characterized by the following aspects, that is a large amount of alcohol consumption relative to other cities high death rate of liver cirrhosis and chronic liver diseases many recipients of the Daily Life Security a large population of aged people. This study was carried out to investigate the interrelation of industrial structure and drinking status of people living under these situations in Kitakyushu. The close association between drinking and liver diseases was found. Furthermore, a large proportion of drinkers were recipients of the Daily Life Security and this is one of the problems to be resolved. It also became clear that it is not easy to stop drinking for habitual drinkers in spite of the presence of liver diseases and the loss of feeling of satisfaction with life which may be triggered by retirement from job possibly hampers them from problem drinking. With an increase of a population of aged, an increase of alcoholism among them may cause serious, local but social problems. These problems may not be resolved only by the effort of clinical medicine, also macroscopical point of views through social structure, industrial structure, a policy of welfare, public health services etc. are required. A part of the study was presented at the 17th, 18th and 19th Conference of Japanese Medical Society of Alcohol Studies.

Adult↗
PubMed1985

[Serum type III procollagen peptide in diagnosis of lung fibrosis due to silicosis and bleomycin toxicity].

Early diagnosis of lung fibrosis has been hampered by the lack of a simple, convenient and specific test. Measurement of serum type III procollagen peptide (Pro (III)-N-P) by the method originally developed by Rohde et al. has been shown to be useful for the evaluation of hepatic fibrosis. The present study, therefore, was carried out to investigate the usefulness of the measurement of Pro (III)-N-P in 24 patients with lung fibrosis due to silicosis, and in 7 patients with malignant lymphoma treated with bleomycin, antitumor antibiotic which was the adverse effect of producing fibrosis in the lung. The normal value of the peptide in adults was 8.60 +/- 2.35 ng/ml (mean +/- SD; n = 68) and the normal upper level was set at 13.4 ng/ml (mean +/- 2SD). Patients with silicosis had significantly but not extremely high levels of the peptide and 25% of the patients showed abnormally high values. The level of Pro (III)-N-P was associated with neither physical findings, chest X-p findings nor pulmonary function test results. Three of 7 patients showed increased levels during treatment with bleomycin. In one case, a total dose of 120 mg of bleomycin for over a period of 14 months markedly increased the level of the peptide. These observations suggest that the determination of Pro (III)-N-P may be useful for the detection of lung fibrosis.

Adult↗
PubMed1983

[Serum type III procollagen peptide in patients with pneumoconiosis].

Early diagnosis of lung fibrosis has been hampered by the lack of a simple, convenient and specific method. Recently Rohde et al. developed an assay method for Type III procollagen peptide. Type III procollagen peptide, an extension peptide released during the biosynthesis of collagen Type III, has been known as a good marker for hepatic fibrosis. Therefore, we applied this assay to the patients with idiopathic pulmonary fibrosis, lung fibrosis in collagen disease and silicosis. And also we measured the serum level of the peptide in the healthy workers being exposed to silica. Normal value of the peptide in adults was 8.3 +/- 2.6 (mean +/- SD; n = 32) ng/ml. The upper value over mean + 2SD, 13.4 ng/ml, was regarded as abnormal value. Six patients with idiopathic pulmonary fibrosis, and lung fibrosis in collagen disease showed a significant increased level of 19.0 +/- 9.6 ng/ml (P less than 0.02). Four of 6 patients had abnormal high values. On the other hand, the patients with silicosis had not so high levels of peptide (12.6 +/- 6.5 ng/ml; n = 24; P less than 0.01), but 25% of the patients showed abnormal values. There were 2 cases among 19 healthy workers being exposed to silica who revealed slight abnormal values, 15.6 and 16.7 ng/ml. These observations suggest that the assay has a prognostic value for lung fibrosis and also is useful for the early detection of active lung fibrosis in the workers, even though Type III procollagen biosynthesis is already low in the late stages of silicosis.

Adult↗
PubMed1983

[Complication of therapeutic transcatheter hepatic artery embolization for hepatoma--case of developing an extensive acute gastric ulcer].

We report the development of an extensive acute gastric ulcer in a 60-year-old man following therapeutic transcatheter hepatic artery embolization for hepatoma. The unintentional occlusion of branches of the right gastric artery with refluxed Gelfoam fragments is thought to be responsible. This complication is probably rare, but should be kept in mind when therapeutic embolization of the hepatic artery is performed.

Acute Disease↗
PubMed1983

Collagenase production by rabbit liver cells in monolayer culture.

The mechanism of collagenase production by cells derived from rabbit liver was investigated in the present study. Fibroblasts alone, second to fourth passage cells, did not produce collagenase even if PMA, a potent inducer of collagenase production, was added to the culture medium. Collagenase activity was detected only in first passage cells, containing hepatocyte-like and fibroblast-like cells, after the addition of PMA. During 9 days of culture, there was negligible collagenase activity in the first 3 days, but in the following 3 days the activity was 2.54 +/- 0.31 U (mean +/- S.E.M., n = 5) (micrograms of collagen degraded per minute) per milligram of cell protein, and in the final 3 days the activity was 9.05 +/- 0.38 U/mg of cell protein. These results suggest that interaction between mesenchymal and parenchymal cells is important for collagenase production in the liver.

Animals↗
PubMed1982

Mammalian collagenase increases in early alcoholic liver disease and decreases with cirrhosis.

To determine if alterations in collagen degradation may contribute to the pathogenesis of fibrosis and cirrhosis, we studied the hepatic collagenase activity of 20 baboons given alcohol containing diets or control diets under carefully controlled experimental conditions. We also studied 28 patients whose livers were biopsied for diagnostic purposes. Hepatic collagenase activity was significantly increased in baboons with fatty liver compared to levels in normal, control fed animals [(1.98 +/- 0.19 vs 1.20 +/- 0.08 units (microgram collagen digested/hour/mg liver protein) +/- S.E.M., p less than 0.001)]. The increase in hepatic collagenase activity persisted at the stage of fibrosis when compared to the activity in control baboons (2.16 +/- 0.07 vs 1.20 +/- 0.08 units +/- S.E.M., p less than 0.001). A single cirrhotic baboon available for study had an hepatic collagenase activity of 1.58 units. Patients with hepatic fibrosis had significantly higher hepatic collagenase activity than those with fatty livers [(9.12 +/- 0.94 (n =14) vs 4.52 +/- 0.54 (n = 7) units +/- S.E.M., p less than 0.001)]. However, in the group with cirrhosis, hepatic collagenase was lower [(3.92 +/- 0.61 (n = 7) units +/- S.E.M., p less than 0.001)] than in the group with fibrosis. Our findings suggest that the development of cirrhosis is coincident with, or favored by a failure of hepatic collagen degradative enzymes to keep pace with hepatic collagen synthesis.

Adult↗