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Biomedical subjects

I O Leitch

Publications and source records attributed to I O Leitch.

9 recordsLinked to original sources

An in vivo model for epithelialization kinetics in human skin.

Epithelialization of wounds has been difficult to measure objectively due to the lack of an adequate model. This paper presents a model for the investigation of epithelialization kinetics in human skin using athymic (nude) rats as an in vivo vehicle. Nude rats are T-cell depleted and as such tolerate xenografts. This technique allows an epithelialization rate for a graft to be calculated by plotting the percentage of its designated analysis area to be closed by neoepithelium over time. This model provides a tool whereby the effect of various agents on the epithelialization kinetics of human skin can be objectively and reproducibly examined. This information may be usefully applied to the clinical management of wounds.

Animals↗

New developments in burn management.

Recent understanding of the effects of topical antimicrobials on cells that heal the wound, the role of infection, and new understandings of the role of occlusive 'biological dressings' have resulted in a more rational treatment protocol for burn wounds. With improvements in the resuscitation and intensive management of severe burns, more patients survive the initial effects of the injury, resulting in developments to close a large burn wound, which include expanding the patient's own skin to cover those large areas.

Bandages↗

Burns to the hand.

Hands are a common area for burns to occur. Small, superficial burns may need little medical care whereas larger, deeper burns require the services of a specialised burn team and a variety of reconstructive plastic surgical techniques to avoid long-term stiffness and minimise functional loss.

Burns↗

Inhibition of wound contraction by topical antimicrobials.

Spontaneous wound healing occurs partly by wound contraction, a process that requires intact functioning fibroblasts, and collagen production. Disruption of fibroblasts by the topical antimicrobials, silver sulfadiazine and mafenide acetate has been demonstrated in vitro. An acute rat wound model was used to show that wound contraction in vivo is significantly impeded by silver sulfadiazine and mafenide acetate.

Administration, Topical↗

Burn wound zygomycosis caused by Apophysomyces elegans.

A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment.

Amputation, Surgical↗

Prevention and treatment of postburn scars and contracture.

The management of postburn reconstruction is complicated by the frequent occurrence of multiple reconstructive needs in a single patient. This article presents a simple, comprehensive approach to burn scar reconstruction. The primary aim of the surgeon is to prevent burn scar deformity by rapid wound closure, correction of tissue deficiencies, and assiduous attention to postoperative splinting and compression therapy. The initial step in managing secondary deformities is to prioritize reconstructive needs. Reconstruction is then carried out in a stepwise fashion aiming to restore active function first, followed by passive function, and finally addressing aesthetic reconstruction. Reconstructive techniques are applied in a hierarchy from simplest to most complex. Primary excision and closure of scars by reorientating the scar to the lines of relaxed skin tension can significantly improve appearance. The use of z-plasty, flap repair, and tissue expansion are also reviewed. Skin expansion, in particular, has become the standard management of postburn alopecia and, although associated with a relatively high rate of complication, has significantly improved the aesthetic appearance of such patients. The management of common problems affecting the face is discussed with particular reference to management of the eyelids, oral commissure, and lips. These areas need to be reconstructed as aesthetic units and each requires individualized management of donor tissue. The reconstruction of the burn patient is often a long process requiring multiple procedures. The approach presented here advocates a stepwise, prioritized approach aiming at both maximum function as well as optimal appearance.

Burns↗