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Biomedical subjects

I Nikolov

Publications and source records attributed to I Nikolov.

At least 37 records · Page 2Linked to original sources

[Comparative studies of hormonal methods for affecting the reproductive functions of cows being raised by different technics].

Studied was the effect of two raising technologies in the industrial rearing of cows (tied and loose housing) along with the effect of the use of hormonal-and-stimulating therapy on their reproductive functions. It was found that the duration of the service period with the two technologies without using hormones was considerably long (respectively 124.3 +/- 7.8 up to 133.0 +/- 5.1 days, and 138.5 +/- 8.1 up to 144.0 +/- 3.3 days). The rate of conception at first insemination with tie-up housing varied from 20.23 to 56.67 per cent, and the total conception--from 74.41 to 86.67, whereas the fertilization of a cow required 2.35 to 2.77 doses of semen. At loose box housing the conception rate varied from 25.13 to 47.69 per cent and 75.15 to 84.61 per cent, and the used doses of semen were 2.40 to 2.69. Treatment with gravohormon between the 40th to 60th day following calving raised the total conception and the conception at first insemination--54.70 and 94.70 per cent at loose box housing, and 62.65 and 95.18 per cent at tie-up housing. Combined hormonal treatment with gravohormon and oroestron within the interval of the 100th-160th day following calving with the two technologies led to the very high clinical manifestation of the estrus, however, it did not raise the conception rate. Treatment with estrumate between the 100th and the 160th day after calving produced a higher effect with regard to conception as against the combined treatment with gravohormon and oroestron. The stimulating hormonal therapy had a higher effect on the conception at first insemination with the technology of tie-up housing.

Animal Husbandry↗

[Dynamics of paraclinical indices in nitrate poisoning in sheep].

Studies were carried out on some paraclinical indices of the blood with sheep in the case of experimental nitrate intoxication. The animals were divided into five groups and were offered nitrates at the rates of 0.4, 0.5, 1.5, and 2.0 per cent of the dry matter in the ration. The feed given to group V was obtained in a natural way (through the overrate dressing of maize with nitrogen fertilizers), while that given to the remaining groups was prepared by adding nitrates in the corn mash. The blood of the test animals was sampled on the 15th, 30th, 45th, 60th, and 75th day to assess the erythrocyte and leukocyte counts and the level of hemoglobin, methemoglobin, blood sugar, carotene, vitamin A, nitrates and nitrites. The leukocyte count was found to drop, and the erythrocyte one -- to rise. Rose also the level of hemoglobin in the sheep of group V. The level of methemoglobin rose proportionally to the increased amount of nitrates swallowed, and the blood sugar was increased with the increase of methemoglobin. Carotene and vitamin A levels dropped, and nitrates rose. No changes were found in the level of nitrites.

Animals↗

[Methemoglobin level in the blood of agricultural animals and poultry].

Studies were carried out with all kinds of farm animals and birds with regard to the quantitative determination of methemoglobin in their blood in connection of seasonal changes. Blood was immediately treated upon sampling. Methemoglobin determination was carried out spectrophotometrically. Evaluated was also the percent of nitrates in the rations of the animals and birds. It was found that during the winter the amound of methemoglobin in the blood of both animals and birds varied from 0.25 to 4.4 of the total amount of methemoglobin. Its percent content during the summer varied from 0.25 to 4.17 with animals, and from 9.5 to 15.7 with birds of the total amount of methemoglobin. The amount of blood methemoglobin was found to correlate directly with the percent of nitrates in the ration. Except for birds, the seasons had no effect on the values of methemoglobin. The breed, weight, age, and physiologic status (pregnancy, barrenness) did not either affect the amount of methemoglobin. There were individual variations of the methemoglobin values with animals of one and the same species.

Animal Feed↗

Distribution of 2,2,6,6-tetramethyl-4-oxopiperidine-1-oxyl free radical in normal and neoplastic animal tissues.

The free radical 2,2,6,6-tetramethyl-4-oxopiperidine-1-oxyl (TMPO) was injected i.p. in doses of 100 mg/kg bw into Syrian hamsters: untreated, partially hepatectomized and grafted with transplantable tumours as well as into mice with B16 melanotic melanoma or with an adenocarcinoma. One hour after application of the compound its content was determined spectrometrically in livers, kidneys, lungs and all tumours. The characteristic triplet signal of TMPO was registered in the hamster and mouse melanotic melanomas but not in all other animal tissues and tumours. This may be the result of an enzyme defect in the melanotic melanoma cells or of a retention of TMPO by melanin. It was also found that TMPO is metabolized predominantly in the livers of the animals.

Animals↗