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Biomedical subjects

I Nath

Publications and source records attributed to I Nath.

89 records · Page 5Linked to original sources
PubMed1977

Circulating T-cell numbers and their mitogenic potential in leprosy--correlation with mycobacterial load.

The effect of treatment and mycobacterial load on circulating T-cell numbers and their functional ability was investigated in forty-one patients with leprosy. Both early binding T cells and their responses to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) were profoundly and uniformly depressed in untreated, and partially treated, bacilleferous lepromatous leprosy (LL) patients as compared with normal subjects and tuberculoid patients. On elimination of mycobacteria, subsequent to chemotherapy, LL patients regain normality in T-cell numbers and their functions. On the other hand, the specific response of lymphocytes to M. leprae did not alter with decrease in mycobacterial load. It appears that the decrease in T-cell numbers and the deficit in their mitogenic potential is a secondary consequence of disease and is related to the antigenic load in patients with lepromatous leprosy.

Antigens, Bacterial↗
PubMed1977

Depression of T-cell function and normality of B-cell response in protein calorie malnutrition.

Antibody response to a T-cell dependent antigen, sheep erythrocytes and to a B-cell mitogen, purified lipopolysaccharide (LPS), has been studied in mice kept on protein deficient (2 and 4 per cent casein) diets. The number of plaque-forming cells (PFC) to SRBC were 20-5 +/- 7-7 per million spleen cells in protein-deficient animals compared to 261-0 +/- 31-1 in parallel controls maintained on a protein rich diet (18 per cent casein). No difference was observed in number of PFC formed in controls and deficient animals to LPS, values were 161-4 +/- 19-7, 158-5 +/- 14-2, & 162-3 +/- 31-9 in control (18 per cent casein) and deficient groups (4 per cent and 2 per cent casein) respectively. The delayed hypersensitivity skin reaction to SRBC measured in foot pads was significantly lower in mice on 4 per cent casein diet compared to controls. These studies suggest that the effect of protein deficiency is primarily on T-cell function and not on the B-cell response;

Animals↗
PubMed1977

Delineation and quantitation of human peripheral blood lymphocyte subpopulations by electrophoretic mobility and role of surface charge in cell to cell interaction.

A modified procedure is proposed for determination of electrophoretic mobility (EPM) of lymphocytes which enables readings on 200 cells from a sample size of 5 x 106 lymphocytes. In normal human peripheral blood, B lymphocytes (bearing surface immunoglobulin and receptor for activated C3) were found to carry the lowest charge (0.94 +/- 0.05 mu/sec/V/cm). Their number on the EPM basis was 16.9 +/- 4.2%. Two subpopulations of T lymphocytes, one carrying high charge (TH) with a mean EPM of 1.26 +/- 0.04 mu/sec/V/cm and the other carrying lower charge (TL) with a mean EPM of 1.11 +/- 0.03 mu/sec/V/cm were discernible. The former subpopulation of T cells formed spontaneous rosettes with SRBC in 10 min and the latter represented the remaining T lymphocytes which formed rosettes with 24 hr of incubation with SRBC. The number of early rosette-forming cells increases from 69 to 89% when T cells were incubated with neuraminidase-treated SRBC. These observations suggest that complementary electrostatic charge on T cells and SRBC are among the forces facilitating formation of rosettes between these cells.

Cell Adhesion↗
PubMed1975

Lymphocytes devoid of T and B cell markers in chronic lymphatic leukemia.

Peripheral lymphocytes of 16 patients with chronic lymphatic leukemia were studied for B and T cell markers with peroxidase-labelled anti-immunogloulin antisera and complement and spontaneous rosetting techniques. Increased numbers of B cells and normal numbers of T cells were noted. A significant observation was the presence of a large proportion of circulating lymphocytes without the conventional markers for T and B cells. This finding is correlated with clinical features.

Animals↗
PubMedDate not supplied

Fibronectin in leprosy lesions: observations using monoclonal antibodies to human fibronectin.

Croystat sections of dermal lesions from 33 untreated patients with leprosy were studied by indirect immunofluorescence using monoclonal antibodies to human fibronectin. Macrophages in borderline (BL) and lepromatous (LL) leprosy showed intense staining with antifibronectin antibodies. In the tuberculoid lesions cells of the mononuclear phagocyte series in an epithelioid cell granuloma stained for fibronectin. The lymphocytes surrounding these granulomas also showed the presence of fibronectin. These results suggest that the granuloma of leprosy consists of macrophages expressing fibronectin and the lymphocytes in the lesion also appear to express this protein. In six borderline cases, the sub epidermal collagen band showed intense staining with antifibronectin antibodies. In addition, the distribution of Ia-like antigens and fibronectin was studied on the plastic adherent cells obtained from peripheral blood of 11 untreated patients with leprosy. Results indicate that higher percentage of adherent cells from lepromatous patients express fibronectin in comparison to adherent cells from tuberculoid patients or controls. However, no difference was observed in the expression of the Ia-like antigens by the adherent cells, from these patients.

Antibodies, Monoclonal↗