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Biomedical subjects

I Nakasono

Publications and source records attributed to I Nakasono.

At least 37 records · Page 2Linked to original sources

[Forensic autopsy--anatomical diagnosis of asphyxial death].

In hospital cases, since a clinical history is usually present and the cause of death suspected, the chief duty of the pathologist is to describe and study the pathologic conditions revealed by his dissection. In medicolegal cases, the forensic pathologist must determine the cause of death and the circumstances of death, without any clinical history or clue to guide him except the lesion of trauma or disease which he discovers post mortem. The recognition of asphyxia as a cause of death at autopsy is occasionally difficult and requires considerable care, skill and understanding based on experience of the forensic pathologist. When a mechanical asphyxial death is suspected, the following method is a convenient routine procedure in our laboratory. 1. External Examination A systematic and careful examination of the external feature is made. 2. Internal Examination A "U" shaped incision is the most satisfactory method to inspect the internal tissues of neck in situ. 3. Laboratory Investigation The tissue examined routinely under the microscope are cervical lymph node, tongue, carotid body, lung, cortex of brain and skin. The heart's blood is collected to examine the fibrinolytic factors and thyroid hormones.

Asphyxia↗

Demonstration of hemoglobin in the kidney of battered children.

We examined specimens of renal tissue taken from 40 cadavers of children (18 battered and 22 non-battered children) by an immuno-peroxidase technique using anti-human hemoglobin (Hb) or anti-human myoglobin (Mb) antiserum. Hb was detected in the proximal tubular cells of the kidney of six battered children who had sustained severe or moderate external injuries, whereas Mb was not found. In contrast, Mb was detected and Hb was not found in the renal tubules of one child who had died from a traffic accident. In other cases including 13 cases of children who died from a sudden bout of physical violence or some other impulsive act and 4 cases of children who had died from malnutrition, neither Hb nor Mb was found in the kidney. Our findings appear to indicate that large quantities of Hb were released from the multiple lesions of the battered children, entered the circulation and were deposited in the kidneys. It thus seems that the immunohistochemical detection of Hb in the kidney may be useful for evidencing the extensive antemortem bruises which are often found in battered children.

Adolescent↗

Serum Gc, Hp and alpha 2HS phenotypes in human T-lymphotropic leukemia virus type I infection.

The serum Gc, Hp and alpha 2HS phenotypes were examined in 64 subjects known to have the human T-lymphotropic leukemia virus type I (HTLV-I) infection and in 60 uninfected subjects. There were no significant differences in the distributions of Gc, Hp and alpha 2HS phenotype and allele frequencies between any grouping of HTLV-I-infected subjects and the controls. No association between the Gc, Hp and alpha 2HS genotypes and susceptibility to adult T-cell leukemia was found.

Blood Proteins↗

Two new orosomucoid (ORM2) variants in Japanese.

Orosomucoid (ORM) of plasma from 287 Japanese was typed by polyacrylamide gel isoelectric focusing followed by immunoprinting with specific antiserum to ORM. Two new variants were observed and they were designated ORM2 16 and ORM2 17.

Gene Frequency↗

[Histological study on possibility of senile dementia diagnosis in forensic autopsy cases].

Forensic autopsied brains from 57 cases were examined to establish histopathological criteria for normal aging and senile dementia. Senile plaques, neurofibrillary tangles (NFT), amyloid angiopathy and lipofuscin deposits were quantified in the occipital lobe and in the hippocampus. Histopathological data were analysed by the image analyzer. The primitive senile plaque (SPP) and lipofuscin deposits were observed over age of 50. The senile plaque with core (SPC) and NFT were presented in the cases above 70 year of age. There were close correlation between the individual age in non-demented, and the number, size and occupation ratio of SPC in the occipital lobe and hippocampus. In the dementia cases, the occupation ratio of SPC in the hippocampus and the number of SPP in the occipital cortex were significantly increased against the non-demented group.

Aged↗

A case of hypoxic brain damage consequent to ligature strangulation.

A case of delayed death following strangulation is reported. A 43-year-old woman died 18 days after being subjected to strangulation. The autopsy and neuropathological findings are presented. Informatively, enzymatic immunohistochemical techniques were used for the neuropathological investigation.

Adult↗

A demonstration of spermatozoa on vaginal swabs after complete destruction of the vaginal cell deposits.

The proteolytic enzyme, proteinase K, has been found to destroy all vaginal cells though it does not have the same effect on spermatozoa. In cases of sexual offenses, in which a swab has been used to wipe out the vagina, the female cells and their nuclei on that swab may also contain the heads of spermatozoa. After as short a time as 30 min of proteinase K treatment, the spermatozoa that had separated from the enzymatically destroyed vaginal cells were recovered. This proteinase destruction furnishes some spermatozoa with deformed heads and a somewhat greater number of isolated tails though a sufficient number of spermatozoan heads still remain for a reliable diagnosis. For detection of spermatozoa from a vaginal swab after proteinase K pretreatment, the heads of the spermatozoa are distinctly stained by Oppitz's method. Further, on prior treatment with proteinase K, the ABO blood grouping of the spermatozoa could also be determined on the vaginal swab by using the absorption-elution technique. The resistance of the spermatozoa to proteinase K is the basis for this method.

ABO Blood-Group System↗

Comparative immunological and electrophoretic analysis of Gc protein in sera from various animals.

1. On immunodiffusion, using an anti-human Gc antibody, serum Gc in all mammals tested revealed a partial identity with human Gc. 2. The relative cross-reactivities of serum Gc in monkeys, dogs, cats and rats with human Gc antiserum were found to be more than 70% while the serum Gc in other mammals (pigs, cattle, goats and a guinea pig) was less than 50%. 3. Testing, using the isoelectrofocusing method, showed specific patterns of Gc in the mammals. In the sera of cats and cattle, Gc polymorphisms were detected. 4. Neuraminidase treatment affected the isoelectrofocusing Gc patterns of pigs, goats and cattle, whereas those in other mammals remained unchanged.

Alleles↗

Two familial cases of dissociation of saliva Lea levels and erythrocyte Lewis types.

Two familial cases in which the saliva Lea levels were seen to dissociate with the donors' red blood cell (RBC) Lewis types are reported. In case 1, the saliva from a donor with an RBC type of Le(a+b-) contained a low level of Lea antigen. A low Lea level was also observed in the saliva from this proband's father who has an RBC type of Le(a+b-). In case 2, the saliva from a donor with an RBC type of Le(a-b+) contained a high level of Lea antigen. High Lea levels were also present in the saliva of this proband's father and brothers with an RBC type of Le(a-b+).

Enzyme-Linked Immunosorbent Assay↗

Detection of Lea substance in saliva stains by enzyme-linked immunosorbent assay (ELISA) using anti-gum arabic serum.

It is known that rabbit anti-gum arabic (GA) serum has cross-reactivity with Lea antigen, and that, by using this cross-reactive anti-Lea antibody, the presence of Lea antigen in red blood cells and saliva can be demonstrated with accuracy. We have devised a rapid and highly sensitive method for detecting Lea substance in human saliva by the enzyme-linked immunosorbent assay (ELISA) method using an anti-Lea antibody isolated from anti-GA serum by affinity chromatography on Synsorb Lea. The ELISA plate, coated with the specific anti-Lea antibody, adsorbed the Lea substance in saliva which was subsequently identified by adding enzyme labeled anti-Lea IgG in that order. The method could detect the Lea substance in Le(a+) saliva stains as small as 0.1 by 0.1 cm in size that had been stored at room temperature for three weeks and in Le(a+) saliva stains 0.7 by 0.7 cm in size that had been stored for ten years. This method seems to be useful for quantitative analyses of the Lea substance in various body fluids.

Alkaline Phosphatase↗

A new hereditary single band variant of the Gc system.

A new single band variant (Gc Ar) or the Gc subtypes not identical with the known Gc variants has been detected in the plasma of a healthy blood donor by isoelectric focusing. Using this technique the variant is represented by a single band which has a similar isoelectric point to the Gc 1C2 anodal band. It is well known that the single band Gc phenotypes remain unaltered after neuraminidase treatment. Nevertheless, the new single band variant (Gc Ar) is altered after neuraminidase treatment as is Gc 2A3. After neuraminidase treatment, the Gc Ar band is affected and moved to the nearby position of the Gc 2 band. Investigation of the proband's family shows that the variant occurs combined with the common alleles Gc 1F, Gc 1S and that it has an autosomal dominant inheritance.

Alleles↗

A new single band variant of the Gc subtypes determined by isoelectric focusing.

A new single band variant (Gc Ta) of the Gc subtypes which does not correspond with the known Gc variants has been detected in a healthy blood donor by isoelectric focusing. Using this technique the variant is represented by a single band, which is more cathodically located than the 1A8(fast) band and more anodically located than the 1A3(fast) band. It is well known that the single band Gc phenotypes remain unaltered after neuraminidase treatment. Nevertheless, the new single band variant (Gc Ta) is altered after neuraminidase treatment. In this case the new single band Gc variant also contained N-acetylneuraminic acid (NANA).

Blood Group Antigens↗