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Biomedical subjects

I Marin

Publications and source records attributed to I Marin.

At least 37 records · Page 2Linked to original sources
PubMed1989

Towards a physical map of the Listeria chromosome: the pulsed field electrophoresis approach.

The possibility of the electrophoretic study of very large fragments of the Listeria chromosome (DNAs up to 1000 Kb) may help to the understanding of the physical organization and functional mapping of the entire genome of this organism. Several experiences were done to apply this technology to Listeria. Agarose inserts were prepared with intact cells and protoplasts, and lysis was induced in situ. Inserts with a convenient amount of DNA were cleaved in situ again by diffusing restriction enzymes into the agarose and submitted to one dimensional electric field that was periodically inverted, to cause changes of direction of the DNA fragments. We obtained a good band resolution of bands with Eco R1 and HindIII. Experiments are in progress to select other restriction enzymes leading to larger fragments. This technique must be combined with the use of blotting with known DNA sequences, such as the corresponding to the haemolysin, to arrive to a comprehensive map of the Listeria chromosome. Cloning of Listeria genes involved in several biochemical characteristics of Listeria, or at least a good collection of mutants will be quite necessary for the progress of such approach.

Chromosome Mapping↗
PubMed1987

[Peritoneal sarcoidosis, a rare localization].

A case of peritoneal involvement in the course of a mediastino-pulmonary and cutaneous sarcoidosis is reported. This form of the disease is rare (8 cases in the literature) and must be distinguished from peritoneal granulomatous reaction to surgical foreign bodies (e.g. talcum powder) or to particles of starch in food. In the case presented here, the patient's surgical history and the presence of birefringent bodies within peritoneal granulomas raise the problem of the link between local granuloma and sarcoidosis.

Adult↗
PubMed1987

[Bronchopulmonary cancers: medical approach of the last weeks of life. Study of 191 patients].

The characteristics of patients with carcinoma of the lung during their last weeks of life were reviewed in 191 patients treated in the same department and who died between September 1982 and January 1985. The main symptoms were breathlessness (112/191) which posed difficult therapeutic problems preventing domiciliary treatment, and pain (87/191) which was more easily managed in the author's experience. The modes of death were also analysed, showing a number of sudden deaths (14). Therapeutic sedatives were the immediate cause of death in 32 cases, in the first part of the study. The authors make a rapid review of the different available palliative measures. This type of study is the only way of changing medical practices and strategies in patients dying in hospital.

Adult↗
PubMed1987

[Mediastinal carcinomas in young men without primary tumors. A chemosensitive entity?].

Three patients out of 99 benefited with a complete remission following chemotherapy with Cisplatinum and Vindesine. The initial characteristics of these patients were studied, they recall a characteristic type of tumour already described and for which a hypothesis of a germ origin has been raised. In spite of their rarity the existence of complete remissions should encourage the use of chemotherapy in such patients who have a "broncho-pulmonary" cancer with a tropism for the mediastinum and without a specified primary tumour.

Adenocarcinoma, Mucinous↗
PubMed1986

[Inoperable non-small cell bronchopulmonary cancers. Results of ambulatory polychemotherapy including cisplatin].

Between October, 1981 and October, 1983, 57 subjects with inoperable non small-cell carcinoma of the lung (localized in 37, metastatic in 20) were treated as out-patients, after oral rehydration, with a multiple chemotherapy regimen which included Cisplatinum, Doxorubicin, Vincristine and Lomustine. No severe haematological or renal reaction was observed. After 2 courses 23 patients were responders (complete, or partial, or minor response), 2 of them with complete response. Patients with localized tumour had complementary radiotherapy, while chemotherapy was pursued in responsive patients with metastasis. Median survival was 9 months in responders and 5 months in non-responders. Thus, chemotherapy including Cisplatinum in medium doses (80 ms/sq.m) can be given to out-patients without risk of renal damage. The results obtained in this study are comparable to those of studies performed with other drug combinations including Cisplatinum.

Adult↗
PubMed1985

[Treatment of pleuropulmonary tuberculosis in 1985].

The treatment of pulmonary TB due to bacilli sensitive to antituberculous treatment should lead to 100 p. 100 therapeutic success. However, this result implies a good bacteriological knowledge of the Koch bacillus, of antituberculous drugs, of therapeutic strategy, of the duration of treatment and, finally, of the reasons for failure. The association of isoniazide (5 mg/kg/day), rifampicin (10 mg/kg/day) and ethambutol (20 mg/kg/day for the first two months) sterilizes tuberculous lesions in 9 months. The introduction of a fourth antituberculous drug, pyrazinamide (35 mg/kg/day for the first two months) enables the duration of treatment to be reduced to 6 months without any loss of efficiency. Tuberculous relapses, sometimes due to polyresistant bacilli, pose difficult problems requiring detailed bacteriological studies of antibiotic sensitivity. Specific measures have to be taken (adaptation of dosage, contra-indications of certain drugs) with respect to the individual patient (elderly patients, pregnant or lactating women). Surgery has a role to play, especially in the treatment of sequellae. Admission to a sanatorium is reserved for infectious cases at the beginning of treatment and for patients living in poor conditions. The cover of social security is essential for successful treatment. Tuberculous is a compulsory notifiable disease.

Aminoglycosides↗
PubMed1984

[Surgically-treated small size non-small cell bronchopulmonary cancers without adenopathies (T1NO). 5-year survival and site of recurrences].

From October, 1976 to February, 1982, 48 patients with T1N0 non-small cell bronchopulmonary carcinoma were operated upon at the Laennec Hospital, Paris. Their characteristics were: mean age 57 years (range: 43-80 years); sex ratio 23; type of surgery: 35 lobectomies, 11 pneumonectomies, 2 bilobectomies; histology: 30 epidermoid carcinomas, 15 adenocarcinomas, 3 bronchoalveolar carcinomas. On 1st January, 1983, 10 patients had relapsed after a mean complete remission period of 20 months (range: 2-29 months); 5 only had a local relapse. The actuarial probability of relapse at 5 years is 45%. Twelve patients died after a median survival of 21 months (range: 0-44 months). Of these, 3 died post-operatively, 8 after relapse and 1 of infarction during a first complete remission. Most relapses involved the mediastinum (50%) and the brain (30%). As the preventive role of mediastinal and cerebral irradiation has now been demonstrated in more extensive forms of non-small cell carcinomas, such irradiations would be justified in the T1N0 forms.

Actuarial Analysis↗
PubMed1984

[Combined treatment of small cell bronchopulmonary cancer. Results of a retrospective study of 59 patients].

Fifty-nine patients with small cell bronchial tumours (36 localized, 17 diffuse, in the absence of marrow biopsy) were treated by a protocol combining chemotherapy and radiotherapy between October 1978 and October 1982. The chemotherapy consisted of three courses of Adriamycin (60 mg/m2 on day 1), Methotrexate (40 mg/m2 on day 2), Cyclophosphamide (800 mg/m2 on day 3), CCNU (60 mg/m2 on day 4). Six patients died during the first month of treatment and can not be evaluated; 53 patients completed the initial course of chemotherapy. The radiotherapy was administered after 3 courses of chemotherapy in 14 patients in complete remission and to 14 patients in incomplete remission with residual thoracic tumour. Of the 22 patients in complete remission following this combined treatment, 8 received a re-induction chemotherapy similar to the induction chemotherapy and 14 were simply followed up. The median follow-up of the survivors is 15 months. The actuarial one year survival rate of the 53 evaluable patients is 35% and the 2 year survival is 9%. There are certain hopes for the future: 1) the actuarial one year survival rate for the 22 patients in complete remission (67%) is significantly higher than that for the 31 patients who did not obtain complete remission (24%); 2) the actuarial one year survival rate for the 8 patients who received re-induction chemotherapy (87%) is significantly higher than that for the 14 patients who did not receive this treatment, although both groups were otherwise comparable. It is therefore possible that multiplication or intensification of the courses of treatment will improve the prognosis.

Adult↗
PubMed1984

[Relationship with the patient with bronchopulmonary cancer. Psychological, family and social aspects].

The relationship with a patient suffering from bronchopulmonary cancer is often difficult for the pulmonary physician. This is because of the gloomy prognosis, the unpleasant reputation of the disease, the length of treatment and its efficacy. The quality of life of the patient depends to a great extent on the manner in which he perceives his illness, participates in the treatment and lives through the various stages of this approach till death. The doctor ought to be the guardian of hope, a hope which changes its form and objective as the disease progresses, all of which lead the patient to an understanding of the gravity of the disease and both enables him to talk about it, while maintaining a confident relationship with the doctor. This double requirement of "truth" from doctor and patient should be present from the onset, till the terminal phase. The whole medical team is concerned with this relationship. A conversation with one person may make the patient fear for any therapeutic hopes, and against those objectives which might have tempered the distress which had risen by an impression of therapeutic impotence. With the family communication is also full of tricky points. The principal preoccupation of the doctor is to preserve the relationship established with the patient. This accompaniment demands great availability and above all a lot of time.

Attitude to Death↗
PubMed1980

[Comparative evaluation of the protective effect of furantril and bloodletting in experimental pulmonary edema].

The author carried out studies on the protective effect of furanthril and bleeding, administered 15 minutes before adrenaline in rabbits with lung oedema induced by adrenaline. The gravity of lung oedema was evaluated by the size of lung coefficient and percentage of the survivals. The obtained results showed that furanthril in a dose of 15 mg per kg showed considerably more manifested effect in comparison with that of bleeding of 7 ml per kg. Their combined usage revealed stronger protective action in comparison with their single application.

Acute Disease↗