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Biomedical subjects

I Macintyre

Publications and source records attributed to I Macintyre.

23 records · Page 2Linked to original sources
PubMedDate not supplied

A new sensitive and fast peptide immunoassay based on enzyme amplification used in the determination of CGRP and the demonstration of its presence in the thyroid.

An enzyme amplified immunoassay for rCGRP based on cofactor cycling has been found to be clearly superior to a comparable radioimmunoassay employing the same antiserum in terms of sensitivity, speed and convenience. Correlation between the two methods was very good. With the enzyme amplified immunoassay we have been able to demonstrate the existence of rCGRP in thyroid extract.

Animals↗
PubMedDate not supplied

Heterogeneity of plasma calcitonin gene-related peptide: partial characterisation of immunoreactive forms.

Calcitonin gene-related peptide (CGRP) in plasma is heterogeneous and derived from both neural tissues and from the thyroid gland in the rat. CGRP is widely distributed in neural and cardiovascular tissues, and also in the thyroid gland in rat. We have previously shown that the main source of circulatory CGRP is the perivascular nerves. The present study shows that, in addition to the neural tissues, the thyroid gland may also contribute to the circulating pool of immunoreactive-CGRP.

Animals↗
PubMedDate not supplied

Effect of prolactin on vitamin D metabolism.

The effect of ovine prolactin on the renal 25-hydroxycholecalciferol-1-hydroxylase was studied in the chick. Prolactin was found to increase the activity of this enzyme in both long-term and short-term experiments. In the long term, 7 days treatment with prolactin caused a marked stimulation of the 1-hydroxylase activity, however this effect was only seen when the enzyme was assayed 2-3 hours after the final injection of prolactin. A single subcutaneous injection of prolactin was also effective in increasing the 1-hydroxylase activity, this effect was maximal at one hour and had largely disappeared 3 hours after prolactin administration.

25-Hydroxyvitamin D3 1-alpha-Hydroxylase↗