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Biomedical subjects

I M Sebetan

Publications and source records attributed to I M Sebetan.

At least 19 recordsLinked to original sources
PubMed1998

Analysis of the short tandem repeat (STR) locus HumVWA in a Qatari population.

Polymerase chain reaction (PCR), amplified DNA fragments and denaturing polyacrylamide gel electrophoresis were used to investigate the STR locus HumVWA in a Qatari population sample (n = 200). Seven alleles were identified with frequencies ranging from 0.0125 to 0.3175. The distribution of genotypes fits the Hardy-Weinberg equilibrium. The HumVWA system shows a power of discrimination of 0.9290 and a chance of paternity exclusion of 60.1% in the population studied.

Alleles↗
PubMed1998

Frequency distribution of D1S80 (MCT118) locus polymorphism in a Qatari population.

The genotype and allele frequencies at the D1S80 locus were determined in a Qatari population sample (n = 300) using the polymerase chain reaction with subsequent electrophoretic separation of the amplified DNA fragments. Twenty-two different alleles containing 15-39 repeats of the basic 16-bp unit and 78 genotypes were distinguished; the alleles with 18 and 24 repeat units were the most common, with frequencies of 0.2117 and 0.4233, respectively. The observed and expected genotype values show no significant deviation from Hardy-Weinberg equilibrium. The power of discrimination for the D1S80 locus in the Qatari population is 0.9137, and the chance of exclusion is 59.01%.

Arabs↗
PubMed1997

HLA DQ alpha genotype and allele frequencies in Qatari population.

Genotype and allele frequencies of HLA DQ alpha locus were determined in a Qatari population sample using polymerase chain reaction (PCR) amplified DNA fragment and hybridization to allele specific oligonucleoid probes in a reversed dot blot format. Among the 200 individuals studied 21 genotypes representing products of 6 HLA DQ alpha alleles have been differentiated, and the frequencies ranged from 0.0775 to 0.2825. The observed and expected genotypes values provide a complete fit to the Hardy-Weinberg equilibrium. The power of discrimination is 0.9321 and chance of paternity exclusion is 60.9% in the Qatari population.

Alleles↗
PubMed1997

An improved high resolution single method for orosomucoid ORM1 and ORM2 phenotyping.

An improved high resolution single method for orosomucoid ORM1 and ORM2 phenotyping using isoelectric focusing (IEF) in wide-scales ultrathin layer polyacrylamide gels of pH range 4.2 to 4.9 is presented. The method is reliable, simple, and provides an alternative for the three currently required ones for typing this genetic system.

Electrophoresis, Polyacrylamide Gel↗
PubMed1997

Genetic polymorphisms of orosomucoid ORM1 and ORM2 in Egyptians, Sudanese, and Qataris: occurrence of two new alleles.

Isoelectric focusing was used to investigate the genetic variants of the human plasma orosomucoid ORM1 and ORM2 gene loci in samples of Egyptians, Sudanese, and Qataris. The study populations were classified into 28 ORM phenotypes determined by 10 ORM1 and 9 ORM2 alleles that included 2 new alleles, designated ORM1*B13 and ORM2*H21. Family studies of these new alleles are in accordance with codominant autosomal inheritance. A new interpretation for two previously reported alleles, ORM1*C6 and ORM2*H17, is also presented.

Alleles↗
PubMed1995

Frequency of D1S80 and HLA DQ alpha alleles in a Chinese population.

Allele frequency distributions for the D1S80 (MCT118) and HLA DQ alpha loci were determined in a Chinese population sample using the polymerase chain reaction (PCR). A total of 25 alleles and 100 phenotypes were observed for D1S80. The frequency of allele 18 was higher than allele 24 only in this Chinese population when compared to other reported populations. A total of 6 alleles and 21 possible phenotypes were observed for HLA DQ alpha. The power of discrimination was 0.97 and 0.93 for D1S80 and HLA DQ alpha, respectively.

Asian People↗
PubMed1993

Transferrin (TF) polymorphism in Libyans.

The genetic polymorphism of transferrin (TF) was investigated in 110 unrelated Libyans, using ultrathin layer polyacrylamide gel isoelectric focusing followed by staining with Coomassie brilliant blue R250. Five common and one rare phenotypes were observed. The estimated allele frequencies were as follow: TF*C1 = 0.7455, TF*C2 = 0.2091, TF*C3 = 0.0409 and TF*D = 0.0045. The theoretical exclusion rate in cases of disputed paternity is 19.2%.

Alleles↗
PubMed1992

PI*E Tripoli: a new allele in the alpha-1-antitrypsin system.

Genetic variants of the human serum alpha 1-antitrypsin (PI system) were analyzed in a population sample of 110 unrelated Libyans. Four common PI M variants and 3 rare ones, including a new anodal variant designated PI E Tripoli (PI ET) were identified. The estimated allele frequencies were: PI*M1 = 0.623; PI*M2 = 0.205; PI*M3 = 0.132; PI*M4 = 0.018; PI*ET = 0.005; PI*S = 0.005, and PI*T = 0.014.

Alleles↗
PubMed1991

Plasminogen polymorphism in Libyans: description of a new rare variant.

The genetic polymorphism of human plasminogen (PLG) was investigated in Libyans using wide-scale ultrathin-layer polyacrylamide isoelectric focusing with subsequent immunoblotting. The 2 common alleles, PLG*A and PLG*B, and 4 previously reported rare ones, PLG*A3, PLG*M4, PLG*B1 and PLG*B2, were observed. In addition, a new intermediate rare allele designated PLG*MTripoli (PLG*MT) was encountered. The estimated allele frequencies for the genes PLG*A, PLG*B, PLG*A3, PLG*MT, PLG*M4, PLG*B1 and PLG*B2 were 0.6409, 0.3091, 0.0182, 0.0045, 0.0091, 0.0045 and 0.0136, respectively. The isolated probability of exclusion in cases of disputed paternity among Libyans is 23.3%.

Alleles↗
PubMed1989

Genetic polymorphisms of orosomucoid ORM1 and ORM2 in a Japanese population: occurrence of new ORM1 alleles.

Genetic polymorphisms of orosomucoid ORM1 and ORM2 in a Japanese population from northern Japan were investigated using isoelectric focusing (IEF) in ultrathin layer polyacrylamide gels containing Triton X-100 and immunofixation. Nine ORM1 phenotypes which are determined by four common and one rare alleles were observed. Two of the identified alleles at this locus were considered to be new. The ORM2 pattern was classified into 14 phenotypes as products of one common and two variant alleles. The estimated allele frequencies were ORM1*1 = 0.668, ORM1*2 = 0.170, ORM1*2.1 = 0.136, ORM1*5.2 = 0.022 and ORM1*7 = 0.004; ORM2*1 = 0.972, ORM2*3 = 0.006 and ORM2*6 = 0.022.

Alleles↗
PubMed1989

Plasminogen (PLG) polymorphism in northern Japanese: confirmation of PLG*M6 allele.

Plasminogen (PLG) phenotyping has been performed on 450 unrelated individuals from northern Japan, using wide-scale ultrathin layer polyacrylamide gel isoelectric focusing combined with immunoblotting. One common phenotype and six rare ones were observed. The rare phenotypes included the recently detected allele PLG*M6 in a new combination with PLG*M5 allele. The estimated allele frequencies for PLG*A, PLG*A3, PLG*M2, PLG*M5, PLG*M6, PLG*B, and PLG*B2 were 0.961, 0.009, 0.001, 0.016, 0.001, 0.003, and 0.009, respectively.

Alleles↗
PubMed1989

Genetic polymorphism of the B subunit of coagulation factor XIII in Libyans: occurrence of a fourth common allele, FXIIIB*6.

FXIIIB phenotypes were determined in neuraminidase-pretreated serum samples by using isoelectric focusing in ultrathin-layer polyacrylamide gels containing 1 M urea and subsequent immunoblotting. In a Libyan population sample from Tripoli, (n = 108) nine different phenotypes as products of four common alleles were recognized, with frequencies as follows: FXIIIB*1 = 0.6574, FXIIIB*2 = 0.2454, FXIIIB*3 = 0.0741 and FXIIIB*6 = 0.0231. It is suggested that FXIIIB*6 is the fourth common allele of the FXIIIB system in this population.

Alleles↗
PubMed1988

Genetic polymorphism of desialyzed alpha 2 HS-glycoprotein by ultrathin isoelectric focusing.

The genetically determined polymorphism of alpha 2 HS-glycoprotein was analyzed by immunoblotting ultrathin-layer polyacrylamide gel isoelectric focusing in the pH range 4-6.5 and neuraminidase pretreated sera. In a Libyan population sample from Tripoli (n = 110) three common phenotypes, alpha 2 HSG 1-1, 2-1, and 2-2, were observed. The allele frequencies were alpha 2 HSG1 = 0.8364 and alpha 2 HSG2 = 0.1636. The theoretical exclusion rate in cases of disputed paternity is 11.8%.

Blood Proteins↗
PubMed1988

A new method for FXIIIA genetic variants determination using isoelectric focusing in 1 M urea. Evidence for three structural gene loci, FXIIIA1, FXIIIA2, and FXIIIA3.

A new method for separating genetic variants of the A subunit of human coagulation factor XIII using ultrathin layer polyacrylamide gel isoelectric focusing in 1 M urea followed by immunoblotting is described. The pattern obtained by this method differs from that reported previously: Three sets of unrelated band patterns are observed and can be explained by the existence of two additional gene loci, designated FXIIIA2 and FXIIIA3, besides the previously reported FXIIIA locus, now renamed FXIIIA1. The FXIIIA2 locus is polymorphic and shows three commonly occurring phenotypes, FXIIIA2 1, FXIIIA2 2-I, and FXIIIA2 2. These are determined by two common alleles, FXIIIA2*1 and FXIIIA2*2, with respective frequencies of 0.7965 and 0.2035 in the Japanese population. The studied population conforms to a Hardy-Weinberg equilibrium, and family data confirmed autosomal codominant transmission. The FXIIIA3 locus is monomorphic.

Alleles↗
PubMed1988

Vitamin D binding protein (Gc) subtypes by isoelectric focusing and immunoblotting.

The polymorphism of the human vitamin D binding protein (Gc system) was investigated in a total of 149 sera from unrelated healthy Egyptians residing in Tanta City, Gharbiya Governorate, Nile Delta of Egypt, using isoelectric focusing (IEF) in thin-layer polyacrylamide gel followed by immunoblotting. The estimated gene frequencies were Gc1s = 0.540, Gc1f = 0.242 and Gc2 = 0.218.

Egypt↗
PubMed1988

A simple pattern method for alpha 2 HS-glycoprotein typing.

An improved method for the separation of the genetic variants of the human serum alpha 2 HS-glycoprotein using isoelectric focusing in ultrathin-layer polyacrylamide gel containing carrier ampholyte pH 4 to 6.5 gradient and neuraminidase pretreated samples is described. The pattern obtained is simple, easily interpretable, and reproducible.

Blood Proteins↗
PubMed1987

Detection of diatoms in blood by a combination of membrane filtering and chemical digestion.

An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 microns in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

Adolescent↗