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Biomedical subjects

I Lutsky

Publications and source records attributed to I Lutsky.

34 records · Page 2Linked to original sources
PubMed1981

Role of the popliteal lymph node in infection with Mycobacterium marinum of the hind footpad of the mouse, and source of the cells that characterize the process.

We examined the possibility that the popliteal lymph node serves as the source of the lymphocytes that, together with macrophages, characterize the lesion produced by infection with Mycobacterium marinum in the hind footpad of the mouse. Naïve mice were partially protected against challenge with M. marinum in the hind footpad by intravenous infusion of lymphocytes harvested from the popliteal nodes of donor mice infected with M. marinum 7 days earlier. Lymphocytes harvested from the popliteal nodes of infected donors, labelled in vitro with 3H-uridine, and infused intravenously into naïve mice that were immediately challenged in the hind footpads with M. marinum, localized in the popliteal nodes of the recipient mice but not in the footpad lesions. Lymphocytes harvested from the spleens of naïve donors and labelled in vitro appeared to home to the popliteal node draining the M. marinum-infected footpad. Thus, the primary role of the popliteal lymph node appeared to be passive trapping of the lymphocytes brought to it by the circulation or afferent lymphatics. We then tried to locate the sources of both lymphocytes and macrophages that characterize the lesion. Temporary occlusion of the abdominal aorta prevented labelling by intravenously infused 3H-thymidine (3H-TdR) of the mononuclear cells of both footpad lesion and popliteal node. Temporary occlusion of the left common iliac artery during 3H-TdR infusion prevented immediate labelling on the ipsilateral side. After 24 and 48 h, however, small numbers of labelled lymphocytes were found in the left hind footpad lesion. Amputation of the right leg at the hip joint, but not right popliteal lymphadenectomy, performed immediately after re-establishment of patency of the left common iliac artery, prevented the late influx of labelled lymphocytes into the lesion of the left hind footpad. Thus, the chief source of both the lymphocytes and the macrophages of the footpad lesion appeared to be the lesion itself.

Animals↗
PubMed1981

Experimental alimentary toxic aleukia in cats.

T-2 toxin was given to cats every other day per os to evaluate the suitability of this species as a model for the human disease, alimentary toxic aleukia. The chronic lethal intoxication resulting was characterized by pancytopenia, hemorrhagic diatheses, bone marrow aplasia, diminished hemostasis, severe lymphatic tissue alterations, and histopathologic changes in proliferative tissue. Clinical signs included vomiting, bloody feces, weakness, lassitude, ataxia, dyspnea, dehydration, loss of weight, and pre-terminal anorexia. The clinical course, hematologic picture, and the gross and microscopic tissue changes seen in the experimentally produced disease in cats were similar to alimentary toxic aleukia, a frequently fatal mycotoxin induced disease of man.

Administration, Oral↗
PubMed1981

Response in the hindfoot pad and popliteal lymph node of C57BL mice to infection with Mycobacterium marinum.

C57BL mice inoculated in the hindfoot pads with 5 X 10(3) viable Mycobacterium marinum developed a localized disease process, characterized by swelling of the foot and increases in the number of acid-fast bacilli and colony forming units recovered. These changes became maximal 10 to 14 days after inoculation and then decreased in intensity. An acute inflammatory response appeared in the hindfoot pad during the first 24 h and increased in intensity during the next few days. By seven days after inoculation, the polymorphonuclear leukocytes had been largely replaced by lymphocytes and macrophages which led, during the next two weeks, to the extensive formation of granulomas. Epithelioid granulomas developed after at least 90% of the organisms had been killed. Simultaneously the popliteal lymph node increased greatly in size due to hyperplasia of the paracortical area, which contained a large number of pyroninophilic cells, and packing of sinusoids with small lymphocytes. Well-formed epithelioid granulomas containing acid-fast bacilli developed in the popliteal node. A small number of viable bacilli were found in both the hindfoot pad and the popliteal node 18 months after inoculation; this was accompanied by solid resistance to secondary challenge.

Animals↗
PubMed1980

Skin test reactivity to dog-derived antigens.

Skin test reactivity to canine antigens was studied by testing atopic patients and veterinarians using a commercially prepared mixed-dog epithelial antigen and breed-specific antigens including dander extracts, serum and urine, obtained from thirty-one different pure-bred dogs. Increased skin test reactivity was noted using breed-specific antigens as compared to the mixed-dog commercial screening extract. Variation in skin test responsivity related to specific breed antigens was also noted. The results suggest that skin tests using canine urine and serum antigens, in addition to the conventional dander antigens, may be useful in the diagnosis of hypersensitivity to dogs.

Animals↗
PubMed1980

Response in distant lymph nodes of mice to infection in the hind footpad with Mycobacterium marinum.

In an attempt to demonstrate the importance of the popliteal lymph node in limiting the progress of infection with Mycobacterium marinum in the hind footpads of C57BL mice, such infections were studied in mice subjected to popliteal or popliteal and inguinal adenectomies. In the absence of the popliteal node, the footpad infection was only slightly enhanced compared with infections of sham-operated control mice; the inguinal node was found to be greatly enlarged and appeared to have substituted for the absent popliteal node. In the absence of both popliteal and inguinal nodes, the disease process in the footpads was again only slightly enhanced, and the axillary node appeared to have enlarged greatly and to have functionally replaced the missing, more proximate nodes. In additional experiments, mice subjected to adenectomy only on one side and injected in that hind footpad with phytohemagglutinin or India ink demonstrated hypertrophy or deposition of carbon particles in the more distant node only on the side of the injection. Thus, there appear to be rather direct functional connections among popliteal, inguinal, and axillary nodes that do not depend on blood circulation.

Animals↗
PubMed1978

A review of allergic respiratory disease in laboratory animal workers.

There is increased recognition of hypersensitivity lung disease among workers with laboratory animals as an occupational disease. Symptoms of asthma in 44 of 78 workers with laboratory animal dander allergy reflected the serious consequences of this occupational ailment. Affected employee profiles induced family history of atopy; immediate (Type I) allergic reaction; symptoms of rhinitis, asthma, and cough; hypersensitivity to one or more species, most often rats, mice, and rabbits. Diagnosis depends on history and physical, radiologic, and laboratory examinations, including skin tests with relevant antigens. Control and treatment depend on environmental change (reemployment or reduction of antigen contact); mechanical devices (masks and filters); chemotherapy (bronchodilators, steroids), prophylaxis and immunotherapy (hyposensitization). Standardization of medico-legal criteria covering occupational asthma is needed.

Animals↗
PubMed1978

Isolation of mycoplasmas from the canine genital tract: a survey of 108 healthy dogs.

Cultivation of preputial or vaginal swab samples from 108 apparently healthy dogs gave presumptive mycoplasma growth in 72 cases. Specific identification of 58 mycoplasma isolates based on biochemical studies, indirect immunofluorescence and growth inhibition tests showed association with eight different species or groups, only four of which had previously yielded genital isolates. Fourteen strains were related to M bovigenitalium.

Animals↗