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Biomedical subjects

I Lind

Publications and source records attributed to I Lind.

At least 91 records · Page 5Linked to original sources
PubMed1980

Uptake and degradation of staphylococcal protein A-IgG and HSA-IgG complexes by peritoneal macrophages from rabbits, guinea-pigs and mice.

Normal rabbit serum formed soluble complexes with protein A(pA) which were not taken up or broken down by rabbit peritoneal exudate (PE) cells. On the other hand, normal guinea-pig and mouse sera formed precipitating complexes with pA which were rapidly degraded by the appropriate PE cells. Rabbit, guinea-pig and mouse antisera towards staphylococcal pA formed precipitating complexes with pA, all of which were rapidly ingested and degraded by the homologous PE cells. Soluble and precipitated IgG complexes were identified by zone electrophoresis in agar with subsequent autoradiography. Precipitating complexes were formed when 125I-HSA was added to homologous rabbit, guinea-pig and mouse antisera, and these complexes were rapidly ingested and degraded by the corresponding PE cells. Thus, staphylococcal pA-IgG complexes (Fc-bound material) are ingested and degraded to the same extent as similar antigen-antibody complexes (Fab-bound material).

Animals↗
PubMed1977

An indirect haemagglutination test for demonstration of gonococcal antibodies using gonococcal pili as antigen. I. Methodology and preliminary results.

An indirect haemagglutination (IHA) test for detection of gonococcal antibodies in human sera was established using purified pili as antigen. The majority of sera (44/51), positive in the gonococcal complement fixation test, were also positive in the IHA test with titres within the range 320-10,000. The results obtained for the following three groups of sera indicate a comparatively high speicificity of the IHA test; 1) 52 sera from children below 12 years of age were all negative, 2) 98% (184/188) of sera from blood donors were negative, 3) only sera from three adults out of 21 patients with meningococcal infections gave a positive reaction.

Adult↗
PubMed1977

Counterimmunoelectrophoresis in the diagnosis of bacterial meningitis.

The aim of the present study was to investigate whether counterimmunoelectrophoresis (CIE) would facilitate the rapid, etiological diagnosis of bacterial meningitis when used in parallel with other routine methods in a medical bacteriological laboratory. Of 3,674 consecutive specimens of cerebrospinal fluid (CSF) received at the Department of Diagnostic Bacteriology, Statens Seruminstitu, 283 specimens (each representing one patient) were selected for examination by CIE on the basis of the following criteria: bacteria or pleocytosis or both by microscopy or positive culture or both. CIE was performed with antisera to Neisseria meningitidis (groups A, B and C), Streptococcus pneumoniae (omni-serum and pools A to 1), and Haemophilus influenzae type b. Antigen was detected in 57% (72/126) of specimens in which cultures revealed these three kinds of microorganisms in CSF and in 12% (17/139) of the culture-negative specimens. CSF specimens from 21 patients with bacterial meningitis caused by other species were all negative in CIE, except four, three of which contained Escherichia coli antigen reacting with antiserum to N. meningitidis group B and one E. coli antigen reacting with antiserum to H. influenzae type b. Specific diagnosis was achieved in 60% (170/283) of the specimens studied and could be extablished within 1 h in 85% (145/170) by the combined results of microscopy and CIE. Ten specimens, nine of which showed a reaction with antiserum to N. meningitidis group A, were positive by CIE only.

Antigens↗
PubMed1976

Sequential studies of lymphocyte responsiveness and antibody formation in acute bacterial meningitis.

Lymphocyte transformation responses in vitro were studied in eight patients with acute bacterial meningitis (in five due to Neisseria meningitidis). Sequential studies were done from 24--48 hr after the first symptoms of infection to complete recovery. In all cases lymphocyte transformation was depressed during the acute phases of illness. The responses to microbial antigens were more affected than the responses to mitogens. The course of the lymphocyte responses to the causative micro-organism showed no difference from the responses to other microbial species. A moderate shift towards increased sensitivity of the lymphocytes to lower doses of the causative micro-organism was observed during the course of illness in three cases. In N. meningitidis infection, a rapid rise was seen in the serum titres of complement-fixing antibodies and in the number of precipitating antibodies, whereas the rise in immunoglobulin concentrations was more prolonged. Characteristic patterns of elevation and return towards normal were found in the serum concentrations of the acute-phase reactants alpha1-antitrypsin, haptoglobin, and orosomucoid. It is concluded that the lymphocyte transformation responses in vitro during severe bacterial infection are largely governed by non-specific factors, and that studies of lymphocyte responses to micro-organisms should always include other microbial species as controls.

Antibody Formation↗
PubMed1975

Protein A reactivity of two distinct groups of human monoclonal IgM.

Fifteen human monoclonal IgM proteins could be divided into two groups based on their ability to inhibit binding of a labelled human monoclonal IgM (IgM-Se) to Staphylococcus aureus Cowan 1 (IgM-Se assay); seven were positive and eight were negative. We found complete agreement between positive reaction in the IgM-Se assay, the presence of protein A reactivity in gel diffusion experiments, and high titres in an indirect haemagglutination test detects antibodies to protein A-IgG complexes. The protein A precipitation patterns of IgM and autologous IgG were not identical.

Bacterial Proteins↗
PubMed1971

[Myxedema coma].

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