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Biomedical subjects

I Kino

Publications and source records attributed to I Kino.

At least 73 records · Page 4Linked to original sources
PubMed1988

[Serial spectral EEG analysis in a patient with non-Hodgkin's lymphoma complicated by leukoencephalopathy induced by high-dose methotrexate].

An autopsy case of disseminated necrotizing leukoencephalopathy in non-Hodgkin's lymphoma associated with high-dose methotrexate (HD-MTX) is reported. The patient was a 5-year-old girl, who was admitted to Hamamatsu University School of Medicine because of an abdominal mass in April, 1984. She was diagnosed as having diffuse lymphoma, medium cell type, according to the LSG classification of a biopsied specimen of the liver mass and great omental lymph nodes. Chemotherapy containing HD-MTX was given until the first bone marrow relapse occurred in September, 1985. Subsequently, the physical and CT findings were normal except for decrement of deep tendon reflex, though slight gait disturbance was apparent. In serial spectral EEG analysis, the alpha/s ratio showed slowing activity upon administration of HD-MTX, and it became irreversible. In June, 1986, second bone marrow relapse occurred, and the patient died in July, 1986. At necropsy, multifocal necroses and extensive spongiosis in the white matter of the parietal and occipital lobes were revealed in the bilateral hemispheres of the cerebrum and cerebellum. Irregular confluent demyelination and necroses were also present in the brain stem, especially the pons. Therefore, serial EEG analysis might be a means of achieving early detection of leukoencephalopathy in HD-MTX-treated patients.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1986

Solid and cystic acinar cell tumors of the pancreas. A report of two cases with immunohistochemical and ultrastructural studies.

Two pancreatic tumors were found in young women without detectable functional symptoms. These tumors were identical in gross, histological and electron microscopic features. Each of them formed a large abdominal mass. One of them was found because of this abdominal mass, while the other was found incidentally. Grossly, the tumors were well circumscribed by a firm, white, fibrous capsule, and their cut-surfaces showed mainly cystic degenerative changes with necrotic and hemorrhagic material, with only some solid portions. The solid portions were composed of uniform cells forming solid and papillary structures with numerous PAS-positive diastase-resistant granules. Immunohistochemical staining for alpha 1-antitrypsin showed a granular distribution. Electron microscopic examination showed that the tumor cells had numerous mitochondria, zymogen-like granules, and annulate lamellae. These findings strongly suggested acinar cell differentiation, although ductal and endocrine differentiation have also been observed by others. Therefore, this type of tumor can best be termed an acinar cell tumor. There was no recurrence in either patient after the operation. Long survival can be expected, but reports indicate that this neoplasm should be recognized as a lesion of low malignancy.

Adult↗
PubMed1985

Parathyroid carcinoma of the oxyphil cell type. A report of two cases, light and electron microscopic study.

Parathyroid adenomas are subdivided into chief cell and oxyphil cell variants. However, the parathyroid carcinomas described thus far have been only of the chief cell type. Two cases of oxyphil cell carcinoma of the parathyroid gland are reported, with light and electron microscopic study. The patients presented apparent clinical hyperparathyroidism with x-ray finding of generalized fibrous osteitis and palpable parathyroid tumors. Initially, a pathologic diagnosis of parathyroid adenoma was made in both of them. However, in due course, pulmonary metastases developed in one patient and a local recurrence occurred in the other, 5 and 8 years after the primary operation, respectively. Review of the microscopic slides showed that both primary tumors met the criteria of parathyroid carcinoma. A matter of interest in both cases is that the neoplasms were composed principally of oxyphil cells. Electron microscopic study confirmed the existence of typical oxyphil cells packed with numerous mitochondria.

Adenoma↗
PubMed1985

[A case of solid and cystic acinar cell tumor of the pancreas].

A 35-year-old woman with a tumor of the pancreatic head is presented. A filling defect, suggesting an extragastric mass, was found in the photofluorogram of a gastric mass survey. But she had no complaints. CT showed a cystic mass in the pancreatic head, but ERCP and celiac angiography indicated no invasive findings. The resected tumor, wrapped well with a partially calcifying fibrous capsule, measured 7 X 7 X 5 cm and was mostly necrotic. On histological examination, malignant findings, were not revealed. PAS-positive granules and zymogen-like granules were found in the cytoplasm by electronmicroscopic examination. Immunohistochemically, alpha 1-antitrypsin granules were detected.

Adult↗
PubMed1984

Promotion of collagen production by human fibroblasts with gastric cancer cells in vitro.

To elucidate the histogenesis of gastric scirrhous cancer, the promotion of collagen production by normal human skin fibroblasts (HSF-1) with human gastric cancer cells (KATO-III, MKN-45 and MKN-28) was investigated by direct coculture and parabiotic culture. Argyrophilic collagenous fibers were demonstrated among fibroblasts on both direct cocultures and parabiotic cultures of the fibroblasts with gastric cancer cells. Microscopic examination showed that these fibers appeared earlier and were more abundant and thicker in direct cocultures and parabiotic cultures than in single cultures of fibroblasts. Gastric cancer cells in single or parabiotic culture did not form argyrophilic fibers. For quantitative proof of the promotion of collagen production by fibroblasts with gastric cancer cells, hydroxyproline produced by fibroblasts was measured. Much higher fibroblast hydroxyproline values were obtained in parabiotic cultures with gastric cancer cell lines than in single cultures of HSF-1. Moreover, the rate of collagen synthesis by HSF-1 was much higher than that of any gastric cancer cell line tested. These results demonstrate that gastric cancer cells enhance collagen production by fibroblasts in vitro. This finding suggests that they may produce a factor promoting fibroblast collagen synthesis and that this may contribute to the formation of stromal collagen in human gastric scirrhous cancer.

Adenocarcinoma, Scirrhous↗
PubMed1984

[Pathology and histogenesis of gastric scirrhous carcinoma].

Gastric scirrhous carcinoma can be tentatively defined as the following. Histologically, poorly differentiated adenocarcinoma, scirrhous type, according to histological classification by Japanese Research Society for Gastric Cancer. Grossly, Borrmann IV type (diffusely infiltrating without ulceration or mass formation). Extensive infiltration of the stomach. Gastric scirrhous cancers were classified as follows. Pyloric stenotic type (6 cases). Cancers were located at the distal pyloric portion, causing stenosis, and considered to originate from the pyloric gland. IIc-erosive type (6 cases). Cancers were located about at the border between pyloric and fundic areas, and considered to arise from the intermediate (or transitional) zone. Rugal type (or Linitis plastica type) (9 cases). Cancers were located at the gastric corpus, causing giant rugae in the fundic region, and considered to originate from the fundic gland. Gastric scirrhous cancers must start at the gastric mucosa as intramucosal cancers, whose histology shows adenocarcinoma mucocellular (signet-ring cell carcinoma). Signet-ring shaped cells in adenocarcinoma mucocellulare disclosed at least three types as follows.

Adenocarcinoma, Scirrhous↗
PubMed1984

Morphogenesis of minute adenomas in familial polyposis coli.

The morphogenesis of minute colonic adenomas was investigated by complete serial sectioning of human tissues from 4 individuals with familial polyposis coli. Minute adenomas, consisting of a few atypical glands, were located in the upper part of the mucosa. Single-gland adenomas, consisting of single atypical glands, also were located in the upper to superficial part of the mucosa with endophytic growth. Twelve lesions smaller than single-gland adenomas also were detected. These lesions, buds of single-gland adenomas, consisting of epithelial clusters with mild atypia, were located in the middle part of the mucosa and had sprouted from normal crypts to the lamina propria. The conclusion was that buds of single-gland adenomas originate in the proliferative zone of normal crypts, sprout out of the lamina propria mucosae, move upward with migration of the epithelial cells of the crypts, and are identified as single-gland adenomas in the upper part of the mucosa.

Adenoma↗
PubMed1983

Evaluation of the new giant-biopsy forceps in the diagnosis of mucosal and submucosal gastric lesions.

In order to improve the diagnosis of gastric disorders on the basis of biopsy material, we devised larger biopsy forceps (5 mm X 2.5 mm in inside dimensions) which fit into the newer fibergastroscope, GIF-1T (Olympus). Samples from 116 cases yielded 276 specimens; 179 specimens (64.8%) showed the full-thickness of the mucosa with muscularis mucosae, and approximately one-third of the total specimens included a considerable amount of submucosal tissue, but none of them contained parts of muscularis propria. In 4 out of 36 gastric carcinoma cases there was inconsistency between endoscopic and histological recognition of the proximal border of carcinomatous infiltration. Differentiation between adenoma and IIa, and benign and malignant lymphoma were more feasible with our forceps. No complications apart from minor bleeding were seen.

Adenoma↗
PubMed1983

Ex vivo autoradiography of the human gastrointestinal tract: a new approach to cell kinetic studies of surgically removed tumor-bearing organs.

Surgically obtained portions of the human gastrointestinal tract were perfused with oxygenated perfluorochemical blood substitute, Fluosol-DA (FDA), containing tritiated thymidine. This procedure of ex vivo autoradiography was performed at the RI Center by perfusion of the isolated organ with FDA from a reservoir 190 cm above the organ through a cannula inserted into the main artery. Microautoradiographic sections revealed that the distributions of labeled nuclei in the normal epithelium of the esophagus, stomach, jejunum, colon and rectum coincided well with those reported previously. However, in the normal colonic mucosa in cases of familial adenomatosis, no expansion or shift of the proliferative zone to the upper part of the mucosa was observed. No significant difference was observed between the labeled figures of normal colonic mucosa of cases of familial adenomatosis and ordinary colonic cancer. This is the first report of ex vivo autoradiography of the gastrointestinal tract of man for studies on cell kinetics.

Adult↗
PubMed1982

Morphogenesis of colonic adenomas in mice treated with N,N'-dimethylhydrazine dihydrochloride.

The morphogenesis of colonic adenomas in ICR mice treated with N,N'-dimethylhydrazine dihydrochloride (DMH) was studied. Treatment of 17 mice with DMH (20 mg/kg/week) for 24 weeks induced 100% incidence of multiple adenomas in the mucosa of the most distal 7 cm of the large intestine. These adenomas were composed of atypical epithelium of the absorptive cell type. Forty-two single atypical gland lesions (single gland adenomas) were detected in the upper part of the mucosa by serial sectioning. These single gland adenomas consisted of atypical epithelium with a narrow lumen and showed endophytic growth to the lamina propria mucosae. Complete serial sections also revealed that single gland adenomas had no direct continuity of the surrounding crypts of Lieberkühn. Single gland adenomas developed into microscopic adenomas consisting of several atypical glands by branching of the small atypical glands.

Adenoma↗