Biomedical subjects

I Kaetsu

Publications and source records attributed to I Kaetsu.

39 records · Page 3Linked to original sources

PubMedDate not supplied

Effect of water soluble polymer, polyethyleneglycol, and glass-forming compounds on cell fusion.

The cell fusion of Molt T-cells was investigated in the presence of water soluble polymer, polyethyleneglycol (PEG) and glass-forming monomers. In cell fusion with PEG only, inactivation of the cell occurred within 2 min. However, in the presence of PEG and water soluble polymer, most of the fused and unfused cells lived even after fusion times of more than 10 min. It was observed that water soluble polymer prevented the inactivation of cells during fusion as a protectant. As the result, ratio of fused cells increased in the presence of water soluble polymers. Some glass-forming monomers used as new fusogens such as M-23G (n = 23) and M-50G (n = 50) monomers having long oxyethylene chains in the methoxypolyethyleneglycol methacrylate, CH3O-(-CH2CH2-O)n-CO-C(CH3) = CH2 as well as PEG #2000 indicated large promoting and protecting effects on cell fusion.

Cell Fusion↗

PubMedDate not supplied

Immobilization of E. coli cell as an antigen by radiation polymerization method.

E. coli NIJ cells were immobilized by radiation polymerization of 2-hydroxyethyl methacrylate at low temperatures. The immobilized E. coli cells as an antigen were reacted with peroxidase labeled anti-E. coli in competition with the free E. coli cells. It was found that E. coli cells can be assayed quantitatively with the immobilized E. coli cells in disc form. Microorganisms such as gram negative bacteria had no specific affinity to anti-E. coli. Cross reactivity of the immobilized E. coli cells with the E. coli cells from various strains was examined.

Antibodies, Bacterial↗

PubMedDate not supplied

Polymer cellulose sheets immobilizing antibody by radiation polymerization.

Antibody such as anti-alpha-fetoprotein was immobilized on cellulose fibril sheets by radiation polymerization of hydrophilic monomers. The nature of the immobilized anti-alpha-fetoprotein sheets was examined by measuring the enzyme activity in an antibody-antigen complex reaction. The relationship between the activity and the polymerization condition was studied. The degree of hydration of the polymer matrix giving a maximum activity appeared to be about 0.2. The activity varied with monomer concentration and with monomer composition in copolymerization of acrylate and diacrylate monomer. The activity was increased by the addition of polyethylene glycol diacrylate monomers, in which the activity increased with increasing length of the oxyethylene unit chain.

Antibodies↗