Search PubMed⌕ Search

Biomedical subjects

I Jurkovic

Publications and source records attributed to I Jurkovic.

At least 19 recordsLinked to original sources
PubMed2004

Immunohistochemical study of melanocytic differentiation antigens in cutaneous malignant melanoma. A comparison of six commercial antibodies and one non-commercial antibody in nodular melanoma, superficially spreading melanoma and lentigo maligna melanoma.

In certain primary and metastatic malignant melanomas diagnostic problems may arise due to their cytologic features and/or absence of synthesis of melanin. As the "classic" combination of S-100 protein and HMB-45 may occasionally fail to stain cells of malignant melanoma, we have tested a series of commercially accessible antibodies which were so far not compared by other authors in the three most frequent subtypes of this tumor. In surgical specimens from 104 cutaneous malignant melanomas (40 nodular melanomas, 46 superficially spreading malignant melanomas and 18 lentigo maligna melanomas) the staining intensity and the proportion of neoplastic cells stained with antibodies to S-100 protein, HMB-45, NKI/C3, NKI/beteb, MART 1 (Melan A), KBA 62 and Mitf was semiquantitatively analysed. The use of this group of antibodies against melanoma-associated antigens revealed it to be a favourable supplement for the bioptical or cytological diagnosis of malignant melanoma in case the traditional/conventional combination of S-100 protein and HMB-45 antibody fails. According to the authors' experience the antibody against KBA 62 has shown to be the most effective antibody followed by the antibodies against MART-1 (Melan A) and NKI/C3.

Antibodies, Monoclonal↗
PubMed2003

Expression of LRP--lung resistance-related protein in the normal colorectal mucosa and in colorectal carcinoma.

Expression of LRP--lung resistance-related protein was analyzed by immunohistochemical staining with monoclonal antibody LRP-56 in 30 specimens (10 from normal colorectal tissue and 20 from colorectal carcinoma). All normal tissue samples were LRP positive. Strong LRP staining was seen in the surface epithelium and upper parts of the crypts. Similarly, colorectal carcinomas demonstrated strong staining for LRP/MVP. Staining pattern of cytoplasm of carcinoma cells was the same as in the normal mucosa. The overexpression of LRP in the colorectal carcinoma may play a role in the process of carcinogenesis or be a marker of an aggressive phenotype. It seems, the high value of LRP is a predictor of response to chemotherapy and prognoses in various tumor types. (Fig. 7, Ref: 18.).

Carcinoma↗
PubMed2002

[Pulmonary thromboembolism over a 25-year period in data from the Institute of Pathology of the P.J. Safarik Medical School in Kosice].

Based on a review of archival autopsy protocols an analysis of the frequency of pulmonary thrombembolism in consecutive autopsy material is presented. The study interval for the analysis was oriented to the period between the years 1949-2000. Our analysis has shown a gradual progression of incidence of pulmonary thrombembolism, as demonstrated in relative figures. The applied preventive measures against the development of thrombosis during hospitalization have not substantially contributed to decreasing its frequency, what is not in accord with some recent observations from other countries.

Autopsy↗
PubMed2001

Extraskeletal Ewing's sarcoma of the nose.

A 20-year-old woman presented with nasal obstruction and slight epistaxis. The obstructing lesion was excised and microscopy showed a neoplasm composed of comparatively uniform undifferentiated cells forming solid nests. The cytoplasm of the cells was clear but poorly demarcated, partly vacuolated and contained much glycogen. Although widespread in the nasal mucosa, the cells did not penetrate into the underlying bone. The cells expressed the MIC2 gene (using the CD99 marker). Electron microscopy showed simple cells with a small number of mitochondria, many glycogen particles; there were no neurosecretory granules present. Early surgical treatment followed by chemo- and radiotherapy have greatly improved the prognosis of EWS: extraskeletal Ewing's sarcoma (EWS/PNET).

12E7 Antigen↗
PubMed2001

Calcium oxalate granuloma of the nose of a chronically dialysed nephritic patient.

The patient was a 54-year-old woman who had been suffering from chronic tubulo-interstitial nephritis for about seven years, requiring haemodialysis. More recently, she developed a polypoid mass in the left nasal cavity causing discomfort on breathing and slight epistaxis. The tumour was of gritty consistency and measured 28 x 8 x 5 mm. Microscopy showed a lobulated almost cystic structure composed of granulation tissue with comparatively few plasma cells and many multinucleated giant cells lining the spaces filled with crystalline deposits of calcium oxalate.

Calcium Oxalate↗
PubMed2000

[Multiple intracranial and intraspinal meningiomas in the neurocristopathy (phacomatosis) type of neurofibromatosis].

Presentation of a case of a "central type" neurofibromatosis in a 56-year old woman, clinically diagnosed erroneously as multiple sclerosis with a 20 years long course. Disturbances of hearing, walking, sight, sensitivity, incontinentia, intracranial hypertension and headache represented the main symptoms. More than 120 intracranial and tens of intraspinal meningiomas represented the leading postmortem finding. In a lesser frequency spinal plexiform neurofibromas and schwannomas were also found. The death was attributed to aspiration purulent bronchopneumonia. Various types of meningioma were seen microscopically, including secretory type and a type with amyloid. Immunostaining was positive with S-100 protein and EMA. Negative expression was found with vimentin, CEA, smooth muscle actin, estrogen and progesterone receptors, amyloid A and cytokeratins. With regard to the presence or absence of key morphological features the presented case was placed according to Sobol et al. (29) into the seventh category of neurofibromatosis (NF7).

Brain↗
PubMed2000

Benign glomus tumor of the superior posterior mediastinum.

An unusual location of a benign glomus tumour, outside of the constantly located regions, e.g. in the subungual location or deeply sited in extremities, was diagnosed in a 56-year-old white female in her posterior upper mediastinum. The single similar case report was published before the era of electron microscopy and immunohistochemistry and single cases of atypical and malignant forms in this unusual location were published only recently. The tumour measuring 5 x 4 x 2 centimeters has caused cough and was associated with occasional righ-sided chest pain. Its rich vascular supply has caused intensive intraoperative bleeding. The postoperative course was uneventful and the patient is free of neoplastic disease or symptoms six years after surgery. Numerous mast cells present within the tumour's interstices must be considered in relation to the possible pathogenesis of the up to now unexplained pain in glomus tumours.

Female↗
PubMed1999

[Immunohistochemical analysis of renal angiomyolipoma].

Immunohistochemical analysis of 6 cases of renal angiomyolipoma not related to tuberous sclerosis proved the same rate of expression of HMB 45 and NKI/C3 as well in epithelioid smooth muscle cells of the tumours. Beyond this, an unspecific expression of alpha 1-antitrypsin and alpha 1-antichymotrypsin was found which has not been mentioned in the literature up to now.

Adult↗
PubMed1999

Enalapril treatment of proteinuria in normotensive children.

A retrospective study was performed in 48 normotensive proteinuric children to evaluate the effect of enalapril (n = 17), a combination of enalapril and prednisone (n = 11) and prednisone alone (n = 20) on urinary protein excretion and systemic blood pressure. Enalapril treatment was associated with significant and persistent diminution of proteinuria from 1.32 +/- 0.23 to 0.53 +/- 0.11 and 0.44 +/- 0.07 g/day on the 4th and 8th week of treatment, respectively. Combined therapy with enalapril and prednisone resulted in a comparable significant reduction of proteinuria from a pre-treatment value of 2.06 +/- 0.42 to 0.63 +/- 0.22 and 0.52 +/- 0.17 g/day on the 4th and 8th week of treatment, respectively. In contrast to this, in the group treated with prednisone alone, proteinuria decreased significantly only from the 6th week of therapy (p < 0.02). Consequently, these children had significantly higher urinary protein losses at the 4th week of treatment as compared to patients on enalapril treatment (given either alone or combined with prednisone) (p < 0.01 and p < 0.05, respectively). Importantly, the enalapril-induced reduction of proteinuria was unrelated to variations in arterial blood pressure and no significant changes in this parameter were observed. The results indicate that enalapril can be used safety and effectively for symptomatic treatment of proteinuria in normotensive children with preserved renal function. ACE inhibitor provides additive antiproteinuric effect to corticosteroids by accelerating the rate of diminution of proteinuria. Its combination with prednisone may be of particular importance in those cases, where the degree of hypoproteinemia is a concern. (Tab. 2, Fig. 1, Ref. 29.)

Adolescent↗
PubMed1999

Ovarian hemangioma.

A rare case report of a clinically asymptomatic hemangioma of the left ovary is described. This tumor was an additional and accidental finding in a biopsy specimen removed because of a medium-sized mucinous cystadenoma in a 32-year-old patient. Immunohistochemically the expressions of Factor VIII and smooth muscle actin and negativity of estrogen and progesterone receptors were found.

Adult↗
PubMed1999

A procedure supplement for tissue processing from percutaneous renal biopsies.

The division of a tissue cylinder from percutaneous renal biopsy into several unequal parts for routine biopsy, ultrastructural analysis, and immunohistochemistry may infrequently cause absence of glomeruli rendering the slides for immunohistochemical staining unsuitable. Instead of previously stained hematoxylin and eosin slide serving for checking up of the presence or absence of glomeruli defrayed by Health Care Insurance Companies the proposed additional control cryostat slide was stained by the Sudan Red stain. Such slides were fully accepted by the Insurance authorities and providing information of the presence and number of glomeruli have eliminated the need for a control hematoxylin and eosin stain. This saved time and expenses and at the same time brought information on the presence, degree and location of steatosis.

Biopsy↗
PubMed1999

[Verification of identification of infectious agents by culture in routine gynecologic cytological screening].

The original Bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. Their diagnosis should be followed by appropriate treatment. Based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-diagnosed as highly possible and recommended for laboratory verification: cocci, Gardnerella vaginalis, Klebsiella, Acinetobacter, Morganella Morgani, Candida, Trichomonas vaginalis, Chlamydia trachomatis and human papilloma virus.

Bacteria↗
PubMed1998

Actinomycosis of the middle ear.

Another case of actinomycosis of the ear is described in a nine-year-old boy, drawing attention to the increasing incidence of diseases of the ear considered to be rare. The patient presented with the clinical signs of chronic purulent otitis media, not responding to conservative treatment. After the diagnosis of actinomycosis was established the patient was treated by surgery and long-term antibiotic medication, responding favourably.

Actinomycosis↗
PubMed1997

[Subacute morphologic changes in the heart after radiofrequency ablation in the area of the atrioventricular junction].

Catheter ablation of the atrioventricular (AV) junction for due to refractory supraventricular tachycardias by means of the radiofrequency (RF) current is at present an accepted and widespread mode of therapy. Although pathomorphological findings of the early postablative period are well documented in animals, only few data are available on pathological postablative changes in humans. In this paper we present the necropsy findings in a woman who suddenly died 25 days after RF ablation of AV junction. In this case the ablative procedure has caused subendocardial necrosis revealing signs of advanced organisation with deposits of lipofuscin and haemosiderin. We have also found the necrosis of fat tissue in the vicinity of the tricuspid anulus in the stage of advanced resorption. The recent complication was the thrombotic occlusion of a small branch of coronary artery in the right atrial posterior wall causing a nonextensive acute infarction. We conclude that our findings are in agreement with the literature data on morphologic similarity of ablative lesions and reparative processes in experimental models and clinical practice. (Fig. 4, Ref. 8.)

Aged↗