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Biomedical subjects

I Ivanov

Publications and source records attributed to I Ivanov.

At least 163 records · Page 9Linked to original sources
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Detection of specific RNA sequences in yeast by in situ colony hybridization.

Recently a convenient method for detection of specific RNA sequences in bacteria has been developed but the original protocol was inapplicable to microorganisms with a rigid cell wall. Here we report a modification of the RNA colony hybridization for use with yeast. The modified method includes the following consecutive procedures: a) treatment of the yeast colonies on the membrane filter with 10% SDS at 65% C for 30 min; b) treatment of the same filter with 3 x SSC, 10% formaldehyde at 65 degrees C for 30 min; c) hybridization with 32P-labelled oligonucleotide (or DNA) specific for the RNA sequence of interest. The intensity of the radioactive signals thus obtained is comparable with that of the E. coli colonies.

Cell Wall↗
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Is short-term training useful in international environmental and occupational health projects? Experience from the Balkans and Latin America.

Short courses/workshops for international training in environmental and occupational health are an important tool for supplementing longer-term training at the graduate-degree level. Considerable collective and shared experiences in short-term training have been accumulated among these 13 projects sponsored by the Fogarty International Center of the National Institutes of Health (NIH), the International Training and Research in Environmental and Occupational Health (ITREOH) program. This article presents general aspects and considerations of short-term training and highlights guidelines drawn from specific experiences with such training activities in Balkan and Latin American countries.

Curriculum↗