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Biomedical subjects

I Ivanov

Publications and source records attributed to I Ivanov.

At least 91 records · Page 5Linked to original sources
PubMed1988

[Comparative study of parathyroid function by serum parathyroid hormone, total adenosine cyclic monophosphate in the urine and its nephrogenic fraction].

The immune heterogeneity of the parathyroid hormone and the possibility of its peripheral activation by 1.25 (OH)2 vitamin D3 hinders the precise evaluation of the functional state of parathyroid glands by direct determination of the hormone in the serum and also the creation of a suppressive test similar to the suppressive tests applied in the study of other hormones. A study was carried out which proves that the indirect examination of the parathyroid function by determining the cyclic adenosine monophosphate in urine and its nephrogenic fraction (i.e. by the effects of the active parathyroid hormone) is of greater importance for clinical practice than the direct determination of the parathyroid hormone in peripheral blood. This is the method of choice in the diagnosis of the subclinical variant of primary normocalcemic hyperparathyroidism.

Calcium↗
PubMed1988

[Echographic diagnosis of diffuse kidney diseases].

In 24 patients with chronic glomerulonephritis the echographic findings were compared with some basic parameters established by histomorphologic examinations. In the echographic examinations 4 degrees of echogenicity were used (Rosenfield, Hrisak). The conclusion is that there is no typical echographic image for the different morphologically defined renal diseases. The cortical echogenicity depends on the degree of interstitial infiltration, the fibrous and sclerotic changes. The echographic image of the kidney is a mixed reflection of the histomorphologic changes of the glomeruli, tubules and interstice. A long-standing hypertension affects the echographic image by increasing the cortical echogenicity. A careful and thorough echographic examination of patients with chronic glomerulonephritis is obligatory but the basic diagnostic method is the needle renal biopsy.

Adolescent↗
PubMed1988

[Lipoprotein and apoprotein changes in type-2 diabetes mellitus].

Lipid metabolism indices were followed up in 62 patients with diabetes mellitus type II and in 20 healthy persons as controls. An increase of serum triglycerides, very low density lipoproteins and low density lipoproteins, a decrease of high density lipoproteins and cholesterol in the upper reference range were found. Following diabetes compensation these indices became normal. In decompensated diabetes apoprotein A increased, apoprotein B decreased. Diabetes compensation lead to normalization of these indices. A comparative study of these indices in diabetics with and without ischemic heart disease was carried out. It showed a considerable decrease of the high density lipoproteins and a parallel increase of the very low density lipoproteins and low density lipoproteins in the diabetics with ischemic heart disease. The results of the study lead to the conclusion that criterion for diabetes compensation should be not only the blood sugar normalization bur also the correction of the lipid fractions changes.

Adult↗
PubMed1988

[Serum osteocalcin level as a marker of the functional state of osteoblasts after oral calcium tolerance test].

The results are presented of an oral calcium tolerance test with 1,000 mg calcium in 20 patients with recurrent renal calcium calculosis, a woman with primary hyperparathyroidism and incipient renal failure (serum creatinine 1.8 mg%), creatinine clearance 55 ml/min) and 9 healthy persons as controls. The serum osteocalcin level was determined before and after the oral test. The results show that the serum osteocalcin level alone is of no differential diagnostic value for differentiation of the various types of hypercalciuria in patients with recurrent renal calcium calculosis. As a marker of osteoblasts functional state however the determination of serum osteocalcin level is of great importance for the early diagnosis of osteoporosis. In 3 patients with renal hypercalciuria, often leading to general osteoporosis, an acute rise of serum osteocalcin level was found after the oral calcium tolerance test. High osteocalcin level was also found in the patient with primary hyperparathyroidism and incipient renal failure.

Administration, Oral↗
PubMed1988

[Parathyroid function in recurrent calcium nephrolithiasis and chronic kidney failure before and after oral calcium loading].

A case is presented of a female patient with recurrent calcium renal calculosis, chronic calculous pyelonephritis and chronic renal failure II degree to whom an oral calcium tolerance test was performed. The data of the test are characteristic for parathyroid hyperfunction. The possible cause of these changed is discussed. The conclusion is reached that the oral calcium tolerance test is of differential diagnostic value in patients with initial renal failure, too.

Administration, Oral↗
PubMed1987

Chemical synthesis and expression in E. coli of a human Val8-calcitonin gene by fusion to a synthetic human interferon-gamma gene.

A gene coding for human Val8-calcitonin (Val8-hCT) was synthesized by the solid-phase phosphite approach and fused to a synthetic human immune interferon-gamma (IFN-gamma) gene. The IFN gene was previously shown to be expressed at a very high level in E. coli [(1986) Gene, in press] due to the control of a strong synthetic promoter and strong ribosome binding site. The cells harboring the fused gene produced 100-150 micrograms per l of bacterial suspension of immunoreactive calcitonin in the form of hybrid IFN-gamma-Val8-hCT protein consisting of 140 amino acids. The Val8-hCT can be released from this protein by CNBr treatment.

Amino Acid Sequence↗
PubMed1987

Chemical synthesis and expression of the human calcitonin gene.

A gene coding for the peptide hormone, human calcitonin (hCT), has been constructed and expressed in bacteria using a prokaryotic vector containing the strong T5P25 promoter and a strong ribosome-binding site. The bacterial hCT is different from the natural hCT by having an additional Met residue at the N terminus and a non-amidated C terminus. Despite these differences, the bacterial hCT is biologically active in rat cells. The results are important for future structure-function relationship studies using mutants constructed by genetic engineering.

Amino Acid Sequence↗
PubMed1987

Inclusion bodies in recombinant E. coli producing human calcitonin tetramer, as visualized by immuno-gold electron microscopy.

Inclusion bodies are described in recombinant E. coli cells harboring plasmid for the expression of a synthetic gene coding for human calcitonin tetramer. The inclusion bodies are visualized by electron microscopy and the protein is identified by immuno-gold technique, using antibodies against synthetic human calcitonin. The diameter of the inclusion bodies is 1 micron on the average.

Calcitonin↗
PubMed1987

[Venous treatment with streptase in acute myocardial infarct and unstable stenocardia (a new method)].

Streptokinase treatment was carried out in the intensive care unit of III Internal Clinic, the Higher Medical Institute--Plovdiv, on 54 patients, 51 of them with acute myocardial infarction (AMI) and 3 with intermediary coronary syndrome, making use of the preparations streptase (GFR) and avelysin (GDR). A control group of 35 patients with AMI was used. The treatment was initiated with stream administration of heparin 10,000 U and urbazon 20 mg, whereafter streptokinase 1,250,000 U per 1 h was included on that background. The results were determined according to ECG in 30 leads, recorded by 2, 4, 6, 12 and 24 h, according to creatine-phosphokinase activity and the outcome of the disease. The treatment was uninterrupted up to 24 h with the determination of the individual fibrinolytic activity. Normalization of S-T segment was established by 4 h with restoration of ECG in 10 patients, and in the rest--maintaining QS or QR forms. Re-perfusion arrhythmias were observed in 66.67% and data about re-thrombosis--in 13.61%. Lethality was 5.89%. The method is emphasized to be a reliable means for the treatment of AMI.

Adult↗
PubMed1987

[Glycosylated hemoglobin (HbA1)--an index in the control of diabetes].

A total of 108 patients with diabetes, type II were studied--68 males and 40 females, with an average age of 63 years for the males and 67 years for the females, with various duration--from freshly diagnosed to over 20 years. A positive correlation was established between the serum HbA1 level and blood sugar before meals, its maximum elevation within 24 h and the evaluation of the stage of decompensation of carbohydrate metabolism. Glycemia and HbA1 are not interchangeable but reciprocally complemented indices for diabetes control, for that subjected to follow up care in particular. The state of lipid disorders in diabetes, HbA1 could be used as a marker of synthesis of HDL cholesterol and to a lesser degree--of triglycerides and total cholesterol.

Aged↗
PubMed1986

RNA colony hybridization method.

A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.

Base Sequence↗
PubMed1986

Excitation-contraction latency in the muscles of children and adults.

Excitation-contraction latency in the fast flexor carpi ulnaris and in the slow triceps surae muscles was studied in healthy children aged one month, 10 months and three years, and in adults. The tests were carried out during isometric twitch following supramaximal indirect stimulation. Excitation-contraction latency was found to increase with age in both the fast and slow muscles. The latency is longer for the slow than the fast muscle. A positive correlation was found in the children between excitation-contraction latency and contraction time.

Adult↗
PubMed1985

Isolation of lambda phage DNA by hydroxylapatite chromatography.

A simple and rapid (1 day) method for preparation of lambda phage DNA was proposed. The method included two main steps: (a) growth and lysis of bacteria containing lambda phage and (b) purification of lambda phage DNA by hydroxylapatite chromatography. The phage DNA prepared by this method was intact and free of RNA, proteins, and bacterial DNA.

Bacteriophage lambda↗
PubMed1985

Comparison of avian MC29 and MC31 viral onc proteins.

Lysates of [35S]methionine-labelled quail cells transformed by MC31 virus were immunoprecipitated with anti-myc or anti-gag serum and analysed in SDS-PAGE. A protein with a molecular weight 110K was found in both immunoprecipitates. Thus, the product of the MC31 genome is a gag-myc fusion protein with the molecular weight as the product of the MC29 genome. Comparison by tryptic peptide mapping and p15 cleavage analysis showed no differences between both P110 proteins.

Animals↗