Biomedical subjects
Publications and source records attributed to I Iuchi.
Explore the source record for details and available documents.
A quadruplicated gamma-globin gene as a (5'-G gamma-gamma-gamma-A gamma-3') was detected in an adult Chinese during a survey designed to detect G gamma- and A gamma-globin genes in Japanese. Five triplicated (-G gamma-gamma-A gamma-) and two single (-gamma-) haplotypes were also detected in 103 healthy adult Japanese. All of the unusual chromosomes appeared to reflect an unequal but homologous crossover between G gamma- and A gamma-globin genes. A new Bgl II polymorphic site located around the 3' terminal region of the G gamma-globin gene was also discovered.
A fast-moving abnormal hemoglobin was detected in a heterozygous condition from a patient with benign goiter. The hemoglobin has a slight but significant high oxygen affinity. The patient does not show any hematologic abnormality. Structural analysis indicated that a lysine residue normally present at alpha 11 was substituted by an asparagine residue. This substitution which has not been described previously was discovered coincidentally by another laboratory (1). The new variant has been named Hb Albany-Suma to denote the origin of the variant in both the United States and Japan.
In the rainbow trout, Salmo gairdneri irideus, round, disc-like erythrocytes in the embryonic circulation (Ery L) are replaced by small, elliptical, disc-like erythrocytes (Ery ImA) after hatching. In the peripheral blood of alevins, Ery ImAs grow into mature, adult erythrocytes (Ery A) of large elliptical, disc-like shape (Iuchi, '73b; Yamamoto and Iuchi, '75). Ery L and Ery A have larval and adult hemoglobins, respectively (Iuchi, '73a). The ontogenetic sequence of hemoglobin switching and erythrocyte replacement during erythropoiesis was examined by o-dianisidine histochemistry as well as immunohistochemistry using FITC-antibody probe specific to adult hemoglobins. The first phase of embryonic erythropoiesis (for Ery L) occurs in the intraembryonic "intermediate cell mass" as well as the extraembryonic blood islands on the yolk sac. This phase of erythropoiesis is transient, continuing during the 7th to 12th day after fertilization and ceasing by the 15th day (5th day before hatching). There is a mixed population of Ery L, ImA, and A in the peripheral blood of posthatching alevins. Ery ImA and A showed immunofluorescence with FITC anti-Hb A antibody but Ery L did not. Erythroid cells, stainable with FITC anti-Hb A antibody, were observed in the kidney and the spleen 1 day before hatching and thereafter, but not in the liver throughout all stages examined. Therefore, we conclude that new erythropoiesis (erythropoiesis for Ery ImA and Ery A) began in the kidney and the spleen 1 day before hatching. These findings indicate that hemoglobin switching during the ontogeny of rainbow trout is based on erythrocyte replacement, correlated with a shift in the site of erythropoiesis from one organ to another.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Two brothers with diffuse palmoplantar keratoderma (Thost-Unna type) also were deaf. Of the 38 members of the patients' family, five had a similar disorder and ten had only hearing loss. The mode of inheritance of the dermatosis is regarded as autosomal dominant and is diagnostically distinguished from the other dermatoses associated with the disturbance of keratinization and deafness. To our knowledge, this is the second report of diffuse palmoplantar keratoderma (Thost-Unna type) with deafness, which is considered to be a new variant of the keratodermatoses.
Explore the source record for details and available documents.
An electrophoretically slowly moving hemoglobin variant, Hb Yusa, was found in a 48-year-old man, his younger sister and mother. No clinical or hematological abnormalities were seen in the individuals heterozygous for this mutant gene. Structural studies of this abnormal hemoglobin showed a new type substitution of beta 21 (B3) Asp leads to Tyr. The percentage of the abnormal hemoglobin in the hemolysate was 40.8. The hemoglobin had neither instability nor functional abnormality.
Explore the source record for details and available documents.
A survey of hemoglobinopathies which was carried out in the Takamatsu district during the period from January to August 1979 detected six families with abnormal hemoglobins. Approximately 6010 inhabitants were screened. Three of these families had the same new Hb variant (Hb Takamatsu beta 120 Lys leads to Gln) that has not been previously reported. Existence of a blood relationship among these three families could not be established even after careful family studies. This abnormal hemoglobin was not associated with adverse symptoms and gave normal hematologic findings in the carriers. The isopropranol test was negative, oxygen affinity was within the normal range, and biosynthetic ratio in reticulocytes was around 1.0. One of the difficulties in the structural analysis of this hemoglobin was related to complete superposition of abnormal beta XT-12b,13 on a beta T-8,9 peptide in the fingerprint of the trypsin digest of aminoethylated aberrant beta X chain. This was overcome by collection of abnormal tryptic beta core (beta XT-10-13) from unmodified beta X chain, and subsequent digestion by chymotrypsin. Edman analysis of the chymotryptic peptides thus obtained successfully confirmed the substitution to be beta 120 Lys leads to Gln.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
An electrophoretically G-like hemoglobin variant, Hb Hoshida, was detected from a ten year old girl and her mother. No clinical and hematological abnormalities were seen in the individuals heterozygous for this mutant gene. Structural studies of this abnormal hemoglobin demonstrated a previously undescribed substitution of beta43 (CD-2) Glu leads to Gln. The proportion of the abnormal hemoglobin in hemolysate was 42.1 per cent. The hemoglobin revealed neither instabilities nor functional abnormalities.
Explore the source record for details and available documents.
Explore the source record for details and available documents.