[Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260].
Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.