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I Iu Safronova

Publications and source records attributed to I Iu Safronova.

3 recordsLinked to original sources

[Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260].

Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.

Alcaligenes↗

[Use of maleic acid by mixed cultures of microorganisms].

In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.

Alcaligenes↗

[Intercellular matrix of Bacillus subtilis 271: polymeric composition and function].

It was found that the intercellular matrix of Bacillus subtilis 271 mainly consists of alpha-1,4-glucan (65% of dry matter), branched at some C-3 residues of the polymeric chain, and poly-D-glutamic acid (19% of dry matter). The kinematic viscosity of aqueous solutions of the native matrix was four times as great as that of deproteinized matrix. Increasing the pH of aqueous solutions of the native matrix from 4 to 6 or decreasing their temperature from 38.5 to 11.5 degrees C caused a three- and sevenfold increase in the viscosity of these solutions, respectively. The integrity of the matrix appears to be essential for maintaining the homeostasis of Bacillus subtilis populations.

Bacillus subtilis↗