[The species composition of associations of causative agents in odontogenic infection and the prospects for antibiotic therapy].
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Biomedical subjects
Publications and source records attributed to I I Oleĭnik.
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Fitness of various commercial nutrient media, manufactured in the USSR, for isolation and cultivation of fermentation-producing actinomyces was under study. The media were tested in two stages. Reference and newly isolated actinomyces cultures were examined at stage 1, clinical material at stage 2. Hottinger's blood agar was found the best for maintaining the growth of facultative anaerobic actinomyces and possessed the highest differentiating characteristics. This medium maintained sufficiently intensive growth of anaerobic actinomyces but only in the presence of meat extract. A liquid nutrient medium was designed, based on Hottinger's hydrolysate, that may be used for studies of these microorganisms' physiologic parameters.
In this work the results obtained in the study of the morphology of 208 Actinomyces strains isolated from the oral cavity and a wide spectrum of their enzymatic activity are presented. The identification of these strains was carried out on the basis of chemotaxonomic criteria. Bacteria belonging to the same taxonomic group were found to have considerable similarity in their morphological and physiological features. On the basis of the data obtained in this study a simplified scheme of the tentative identification of fermentative Actinomyces, suitable for use by a wide circle of researchers, is presented.
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Mixed microbial associations from purulent foci of 30 patients with odontogenic inflammatory diseases were studied. 18 specimens of the clinical material were subjected to three-fold investigation. Bacteroids, propionic bacteria and pentococci predominated in the mixed cultures. Nonpathogenic Neisseria, streptococci of the mitis-salivarius group and epidermal staphylococci were isolated from the facultative anaerobic flora. The anaerobic species were the most sensitive to the antibiotics used in the treatment of the patients.
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No less than 4-fold increase in the antibody titres to Pseudomonas aeruginosa during the infectious process served as laboratory confirmation of its participation in the infectious process. In 49 of 91 patients hemagglutining titre exceeded the diagnostic one (1:640). In 9 patients (chiefly in infants) hemagglutinin titre remained low, but there was a rise of antibody level to Pseudomonas aeruginosa during the disease. High hemagglutinin titres were noted in 12 patients on admission to the clinic, with reduction of the antibody titres at the late periods of the disease. Antibody titres remained unchanged during the disease in 15 patients. In 3 cases the indirect hemagglutination test was assesed as negative. In the rest of the patients hemagglutinin titres varied within the range of the diagnostic titre. Thus, the indirect hemagglutination test with erythrocytic diagnostic agent permitting to determine antibodies to Pseudomonas aeruginosa could be used at the clinic in combination with bacteriological and other investigations for establishing etiology of the destructive process.
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