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Biomedical subjects

I Husain

Publications and source records attributed to I Husain.

60 records · Page 4Linked to original sources
PubMed1972

Influence of nutrition on the morphology of a strain of Bifidobacterium bifidum.

A mucoid variant of Bifidobacterium bifidum was converted from its normal curved rod or bifid form to a highly branched form when grown in a chemically defined minimal medium. Branching could be prevented by the addition of a mixture of dl-alanine, dl-aspartic acid, l(+)-glutamic acid, and dl-serine, but not when any one of these four amino acids was omitted. Although sodium chloride induced pleomorphism, calcium ions were ineffective in suppressing the appearance of these pleomorphic forms. None of the cell wall precursors tested, viz., N-acetyl-d-glucosamine, alpha-epsilon-diaminopimelic acid, and muramic acid, inhibited branching.

Acetates↗
PubMedDate not supplied

Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins.

The ABC excision nuclease of Escherichia coli is an ATP-dependent DNA repair enzyme composed of three protein subunits, UvrA, UvrB and UvrC. The DNA sequences of all three genes have been reported. UvrA, the component that binds directly to the DNA, and UvrB, which attaches itself to the UvrA-DNA complex, both contain consensus sequences though to be diagnostic of ATP-binding sites, although the UvrC sequence does not. We now report that a computer analysis of the UvrA sequence has revealed an unusual series of internal duplications centering around putative metal-binding sites which may be involved in the interaction with DNA. We also find a strong evolutionary relationship to a family of prokaryotic membrane-associated active-transport proteins.

Amino Acid Sequence↗
PubMedDate not supplied

Epinephrine and fentanyl as adjuvants to 0.5% bupivacaine for epidural analgesia.

The analgesic and respiratory effects of epidural 0.5% bupivacaine were studied in four groups of patients (n = 170) undergoing percutaneous nephrolithotripsy (PNL). Group 1 patients (n = 42) received 0.5% bupivacaine. Group 2 patients (n = 42) received 0.5% bupivacaine with 1:200,000 epinephrine. Group 3 patients (n = 42) received 0.5% bupivacaine plus 100 micrograms epidural fentanyl, and Group 4 patients (n = 44) received 0.5% bupivacaine plus fentanyl plus epinephrine. Intraoperative pain occurred in anatomically adequate segmental blockade in 15 patients from Groups 1 and 2, but in none from Groups 3 and 4. Analgesia was restored by 100 micrograms epidural fentanyl, and motor block appeared to be enhanced. The addition of epinephrine increased the intensity of motor block but not analgesia. Respiratory frequency was slower and respiratory rhythm more irregular in Group 3 than within the other groups. SaO2 decreased to 89% or lower in eight cases, and oxygen supplementation was required. It is concluded that PNL may be safely conducted under epidural analgesia when reinforced by epidural fentanyl while breathing air, provided SaO2 is monitored continuously; otherwise, oxygen supplementation should be provided.

Adjuvants, Anesthesia↗