The influence of single (4 mg/mouse) and multiple (1 mg/mouse per day for 5 consecutive days) injections of PGM on some hepatic enzymes, lipid peroxide generation in serum and liver, sialic acid concentration in serum and spleen and hepatic lysosomal membrane permeability was investigated. The studies performed showed that a single injection of PGM in vivo changed temporarily the permeability of lysosomal membranes, lipid peroxidation products and sialic acid concentration, and when administered in vitro modulated superoxide anion production and did not affect the activity of lysosomal membrane enzymes. Multiple injections of PGM did not cause significant changes in the examined parameters. Although the metabolic changes were time-limited and from the toxicological point of view, provoked transient effects, the results obtained may be of importance when using PGM in combined chemo-immunotherapy.

Acetylmuramyl-Alanyl-Isoglutamine↗

PubMedDate not supplied

Influence of methionine-enkephalin on stress-induced parameters.

This study examines the influence of methionine-enkephalin (MENK) on stress-induced oxidative damage (lipid peroxidation; LPO) in mice liver homogenate, plasma corticosterone concentration (CS) and phagocytic activity of mouse splenocytes. The LPO value increased in the mice subjected to restraint stress and had no correlation to stress duration, while MENK had no effect. The CS concentration was enhanced after 6 h of stress and 6 h after injection of a low (2.5 mg/kg bw) dose of MENK. However, MENK and stress are adjunct modulators of LPO and corticosterone in vivo. LPO was additionally elevated when MENK (10 mg/kg bw) proceeded for 2 h after the onset of stress. However, corticosterone concentration seems to be regulated differently by the same dose of MENK depending on the duration of stress i.e. elevated in cases involving short periods of stress (2 h) and decreased in cases involving prolonged periods of stress (6 h). This modulation of LPO and corticosterone by 10 mg/kg bw of MENK and 2 h of restraint stress was paralleled with elevated phagocytosis.

Animals↗