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Biomedical subjects

I Hiroto

Publications and source records attributed to I Hiroto.

36 records · Page 2Linked to original sources
PubMedDate not supplied

Establishment of a new cell line from maxillary sinus carcinoma.

We succeeded in deriving a long-term tissue culture cell line from human maxillary sinus carcinoma. This cell line, designated as MC, was passaged 100 times in vitro over a period of 18 months. The cells are globular in shape, grow as single cells in the culture medium, and the mean population doubling time is about 12 hours. The plating efficiency rate in soft agar is 78% and chromosomal analysis revealed the modal chromosome number to be between 47 and 51. These MC cells were transplanted into five nude mice, all of which developed a tumor after a latency period of 5 to 8 days and died within 39 days. Complete autopsy of all mice revealed no metastasis. Histopathological findings of the original and the transplanted tumor tissues showed a remarkable similarity.

Aged↗
PubMedDate not supplied

Immunopathological study of the larynx. IgA distribution and secretory activity.

Using immunofluorescent and histopathological techniques, we studied the distribution and secretory activity of the glands of the larynx from 55 humans. The cavity of the larynx was covered with secretory glandular cells with the exception of the true vocal cords, aryepiglottic folds and upper part of the laryngeal surface of the epiglottis. Immunofluorescent study revealed the presence of IgG and other immunoglobulins throughout the connective tissue ground in each laryngeal mucosa. The most striking was seen in the staining pattern of IgA. Diffuse fluorescence for IgA was observed not only in the submucosal or periglandular connective tissue area but also in the intraeptithelial or submucosa glands. Secretory component synthesis was found mainly in each serous-type glandular epithelium or acinus. In the submucosa or periglandular area, IgA producing plasma cells were numerous. Our observations suggest that IgA, produced locally, is secreted via the glandular cells as secretory component--SIgA. Secretory activity of SIgA in these glandular tissues indicates that the larynx is possibly an immunologically active organ in the upper respiratory tract.

Adolescent↗