Search PubMed⌕ Search

Biomedical subjects

I Hayakawa

Publications and source records attributed to I Hayakawa.

At least 55 records · Page 3Linked to original sources
PubMed1989

Rerupture of intracranial aneurysms during angiography.

We analyzed 70 patients (64 from the literature and 6 of our own cases) who had suffered from rerupture of their aneurysms during angiography. When these cases are compared with those who had suffered rupture of their aneurysms only once and a rerupture, which did not coincide with angiography, they were clinically distinguished by a higher Hunt-Hess grade, a higher rate of IC aneurysms, less operability, far miserable outcome and concentration of aneurysmal rerupture within three hours after the initial subarachnoid haemorrhage. It is suggested waiting at least 3 hours after SAH before performing angiography and to use digital subtraction angiography in order to prevent aneurysmal rerupture during angiography.

Adult↗
PubMed1989

Nephritogenic glycoprotein. XIII. Isolation and its immunochemical characterization of the antigenic substance of membranous glomerulonephritis induced by a single injection of crude nephritogenoside in homologous animals.

Studies on the immunochemical characterization were made on the antigenic substance of membranous glomerulonephritis (MG) that is induced, in homologous animals, by a single footpad injection of rat crude nephritogenoside (CNG). In rats injected with rat CNG, typical MG with immunofluorescent granular pattern (immune complex type nephritis) was produced 3-4 months after the injection. Since CNG nephritis is a model which induces MG only in homologous animals, the development of MG in this model should be explained by antigen-autoantibody immune complex mechanism. Thus, the features of the antigenic substance contained in CNG was immunochemically investigated, utilizing the serum (autoantibody) of rats with MG as well as rabbit antiserum to rat tubular brush border antigen (FXIA) (heteroantibody). As a result, two different fractions (fr. 31 and fr. 36) were separated from the antigenic substance contained in CNG: that is, fr. 31 reacts only with rabbit antiserum to rat tubular brush border antigen (FXIA) (heteroantibody) but does not react with the serum (autoantibody) of rats with MG. Fr. 36 does not react with rabbit antiserum to FXIA (heteroantibody) and reacts only with the serum (autoantibody) of rats with MG (which was induced by a single footpad injection of CNG or soluble FXIA). These results suggest immunologically that fr. 36 is the main antigen responsible for the development of MG which is induced in homologous animals by a single footpad injection of CNG.

Animals↗
PubMed1989

[Development of a light curing denture base resin].

We developed the light cured direct relining material and used it clinically. In this study, a partial change in its components was made to improve both its strength and manipulability with the aim of using it as a denture base material. The physical properties of the new material, bonding to resin teeth, fit, as well as procedure were discussed. As for physical properties, the bending strength of the new material was increased by improving its organic filler, it becoming comparable to that of heat curing resin. The water absorption of the new material was the lowest of compared materials. The bonding strength of this material to resin teeth was increased by treatment with a light cured bonding agent and the fit of the new material to a test model was comparable to that of heat curing resin. Thus, the new material seems to be quite clinically effective as a denture base resin.

Composite Resins↗
PubMed1989

Role of dipeptidyl peptidase IV (gp 108) in passive Heymann nephritis. Use of dipeptidyl peptidase IV-deficient rats.

Injection of antibodies to renal tubular membrane (Fx1A) into Lewis rats induces granular deposits of IgG in glomeruli and proteinuria (passive Heymann nephritis, PHN), and similar lesions are also induced by antibody to one of the antigens in Fx1A, dipeptidyl peptidase IV (DPP IV, gp 108). In this study, the role of DPP IV in PHN was investigated using DPP IV-deficient F344 rats. The amount of DPP IV found in F344 rat kidneys was less than 0.05% of that present in Wistar rats, and injection of anti-DPP IV antibody into F344 rats did not induce proteinuria. Injection of anti-F344 Fx1A rabbit antibodies that contain no detectable anti-DPP IV antibody into Lewis or F344 rats induced PHN, characterized by granular deposits of rabbit IgG in glomeruli and massive proteinuria, although the appearance of proteinuria was delayed in comparison with that occurring in response to injection of anti-Wistar Fx1A antibodies. These results indicate that DPP IV may contribute to, but is not essential for, the induction of PHN.

Animals↗
PubMed1989

[Craniopharyngioma with malignant transformation--a report of two cases].

Two cases of a craniopharyngioma with malignant transformation are reported. Case 1 involved a 3-year-old male who had received a partial resection and radiotherapy for a suprasellar tumor. Histologically, a biopsy specimen showed craniopharyngioma. Eight years later, the child died of an intracerebral and nasopharyngeal invasion of the recurrent tumor. Case 2 involved a 9-year-old male who initially had been diagnosed as having a craniopharyngioma in the suprasellar region. Five years after the first operation, he died from growth of the tumor in spite of radiotherapy and a partial resection. The pathological examinations of these two cases showed an apparent transition of the craniopharyngioma into a squamous cell carcinoma.

Ameloblastoma↗
PubMed1987

Inhibition of DNA gyrase by optically active ofloxacin.

Inhibition of DNA gyrase activity by optically active ofloxacins was studied and compared with the inhibition of norfloxacin and ciprofloxacin. The (-)-isomer of ofloxacin inhibited the supercoiling activity of gyrase from Micrococcus luteus more effectively than did the (+)-isomer. The 50% inhibitory concentrations of (-)-, (+/-)-, and (+)-ofloxacin; norfloxacin; and ciprofloxacin for gyrase from Escherichia coli were 0.78, 0.98, 7.24, 0.78, and 1.15 microgram/ml, respectively. These values correlated well with the antibacterial activity of each compound against intact bacterial cells.

Anti-Bacterial Agents↗
PubMed1987

Heymann nephritis in rats induced by human renal tubular antigens: characterization of antigen and antibody specificities.

An injection of human renal tubular fraction, Fx1A, with Freund's complete adjuvant in rats induces Heymann nephritis (HN) with clinical and morphological features similar to rat Fx1A-induced HN. The present study was undertaken to characterize antibody specificities and the corresponding antigen involved in human Fx1A-induced HN. In sera of rats immunized with human Fx1A, not only anti-human Fx1A antibody but also antirat Fx1A antibody was detected by enzyme immunoassay. Eluted antibody from the glomeruli also showed both activities. In an inhibition study of enzyme immunoassay, binding of the eluted antibody to human Fx1A was inhibited by both human and rat Fx1A and also by partially purified gp330, but not by purified gp108. Furthermore, binding of the eluted antibody to rat Fx1A was inhibited by both rat and human Fx1A. On immunoblot, the eluted antibody mainly reacted with a 440 kD glycoprotein of human Fx1A which showed the same mobility as rat gp330. The antibody also reacted with a band of gp330 of rat Fx1A. These results indicate that the 440 kD glycoprotein in humans is a counterpart of rat gp330 and that the eluted antibody has dual specificities to both human 440 kD glycoprotein and rat gp330, suggesting that the cross-reactive antibody is responsible for human Fx1A-induced HN.

Animals↗
PubMed1987

Identification of gp108, a pathogenic antigen of passive Heymann nephritis, as dipeptidyl peptidase IV.

Our previous paper showed that the glycoprotein of Mr 108,000 (gp108) is one of the major components of rat renal tubular brush border antigens and that an injection of anti-gp108 antiserum in rats induces passive Heymann nephritis with acute and severe proteinuria. In this study, gp108 was identified as a monomer of dipeptidyl peptidase IV (DPP IV). The anti-gp108 antibody was shown to immunoprecipitate DPP IV from Triton-extract of renal tubular membrane fractions. DPP IV was co-purified with gp108 from the Triton-extract by columns of DEAE-cellulose and Bio-gel A-1.5 m. The ratio of DPP IV activity and gp108 content was nearly constant throughout the purification steps. The final purified gp108 showed a high specific activity of DPP IV, comparable to that reported for purified rat DPP IV. These results indicate that gp108 is a monomer of DPP IV.

Animals↗
PubMed1987

The detection and characterization of renal brush border antigen (gp108) in various rat tissues.

We recently reported that an injection of antibody raised against renal brush border glycoprotein, gp108, in rats induced passive Heymann nephritis with acute and severe proteinuria. In this study, the distribution of gp108 in various rat tissues was investigated. On enzyme immunoassay, anti-gp108 antibody reacted to extracts of small intestine, lung, spleen, thymus, liver, epididymis, stomach, pancreas and heart. It also reacted to sera and extracts of peripheral blood cells, especially lymphocytes. These antigens, which are cross-reactive to kidney gp108 have similar biochemical properties: a molecular weight of about 108,000 and an isoelectric point of 4.8-5.4. These results show that immunologically and biochemically related antigens to renal glycoprotein, gp108, are present in various rat tissues.

Animals↗
PubMed1986

Synthesis and antibacterial activities of optically active ofloxacin.

Two optically active (100% enantiomeric excess) isomers of ofloxacin [(+/-)-ofloxacin; DL-8280; (+/-)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7 H-pyrido[1,2,3-de] [1,4] benzoxazine-6-carboxylic acid] were prepared by use of their optically resolved synthetic intermediates. One of the isomers, (-)-ofloxacin, was 8 to 128 times more potent in inhibiting the multiplication of gram-positive and gram-negative bacteria than the other, (+)-ofloxacin, and approximately two times more active than the racemate, (+/-)-ofloxacin.

Chemical Phenomena↗
PubMed1986

Passive Heymann nephritis with acute and severe proteinuria induced by heterologous antibody against renal tubular brush border glycoprotein gp108.

Passive Heymann nephritis with acute and severe proteinuria was produced in rats by a single injection of heterologous antibody against a purified glycoprotein which consisted of homologous subunits with a molecular weight of 108,000 (gp108). Gp108 was identified as one of the major antigens in rat renal tubular fraction (FX1A) on immunoblotting assay by using total proteins of FX1A and rabbit antiserum against FX1A. A band of gp330, which was identified as a pathogenic antigen of Heymann nephritis by Kerjaschki D and Farquhar MG (Proc Natl Acad Sci USA 79:5557, 1982) was detected as another band by Coomassie blue staining and immunoblotting. Autoantibodies in the sera of FX1A-injected (active Heymann nephritis) rats reacted to the band of gp330 but not to gp108. These results indicate that gp108 is a different glycoprotein from both gp330 and its degradation products. GP108 was subsequently purified to near homogeneity by extraction with Triton X-100, and then DEAE-cellulose and Bio-Gel A-1.5m column chromatographies. On gel permeation chromatography, the purified antigen showed a molecular weight of 310,000, suggesting that it consists of dimer or trimer of gp108. Rabbits immunized with gp108 produced an antibody which showed monospecific binding to gp108. The antibody stained with brush border of proximal renal tubules in addition to the capillary loops in rat glomeruli by indirect immunofluorescence. Injection of rabbit antiserum against gp108 in rats induced severe proteinuria within 2 days. On the 2nd day after the injection, the glomeruli of the animals showed granular immune deposits along the capillary loops in addition to dominant staining of the brush border of the proximal tubules by immunofluorescence. These results indicate that gp108 is a pathogenic antigen in passive Heymann nephritis and that an antibody against gp108 has a nephritogenic and proteinuria-inducing activity.

Acute Disease↗
PubMed1986

[Cerebral ischemic lesions in children and primary cerebral angiitis: report of two cases].

Case 1. A seven-year-old boy was admitted to our hospital shortly after developing right hemiplegia and motor aphasia during rope-jumping play. Neither cervical trauma nor signs of cervical and pharyngeal inflammation were present. The physical examination was negative. The carotid angiogram on the day of admission gave poor visualization of the middle cerebral artery group, although the computerized tomographic finding was not remarkable. His neurological status gradually got better, while the routine laboratory studies gave only a slight increase of ESR (26 mm/hour) and positive CRP(I+). There were no particular findings of CSF from lumbar tap or of serological studies. The second computerized tomographic scan 42 hours after clinical onset showed a wide low dense area with obscure margin in the left parieto-temporal region, which was enhanced variously with intravenous contrast media. In about ten days his clinical state was remarkably improved. The second carotid angiogram one month after admission showed a poor filling of the middle cerebral artery territory and beaded appearance of the artery. The patient was discharged on the 46th day with a minimum right hemiparesis. Case 2. A ten-year-old boy was admitted with complaint of right hemiparesis and motor aphasia developing at night. The physical examination was negative. The laboratory studies were also negative. The computerized tomographic scan five days after onset showed a low dense area in size of 2 x 1.5 x 2 cm in the region of the left Nucleus lentiformis. No enhancement effect was seen. The left carotid angiogram on the same day revealed stenotic lesions at the M1 and the posterior temporal artery.(ABSTRACT TRUNCATED AT 250 WORDS)

Arteritis↗
PubMed1985

[Traumatic delayed intracerebral hematoma--clinical aspects].

In order to seek the occasion and the management of traumatic delayed intracerebral hematoma, we selected 17 cases (4.2%) of traumatic delayed intracerebral hematoma (secondary lesion) from 401 cases of head injury, and compared them with other 11 acute traumatic intracranial multiple lesion (immediate lesion). The age ranged from 15 to 78 (except for a newborn hit on the cranium by his mother) and the average was 49 in secondary lesion and 58 in immediate lesion. Sex distribution, 15 males and 2 females in secondary lesion, and 10 males and 1 female in immediate lesion. Though both lesions were supposed to be resulted from almost same severe loads, secondary lesion took worse prognosis than the other: misery outcome (poor and fatal) of secondary lesion was 59% and that of immediate lesion 27%. Of 8 hypertensive cases who were all fatal, 7 were of secondary lesion. Secondary lesion could be expected in only 59% (10/17) by the initial X-CT performed 5.4 +/- 6.2 hr. after trauma. The predicted secondary lesion were classified into three types: salt and pepper like high density lesion (Lanksh's II type), low density lesion (Lanksh's I type) and isodensity mass lesion. The shortest interval between injury and the X-CT detection of secondary lesions was 10 hours and the longest interval was 69 hours (40 hours mean) in conservatively treated patients. In operated patients, the shortest and longest intervals were 8 and 84 hours, respectively (33 hours mean, except for one case who was not examined for 10 days after operation).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1985

[Study of ruptured intracranial aneurysms with intracerebral hematomas--with special reference to operative indication].

We reviewed 40 cases of intracranial ruptured aneurysm with intracerebral hematoma (An-ICH) and compared those with 177 cases of intracranial single ruptured aneurysm causing only subarachnoid hemorrhage (An-SAH). An-ICH accounted 11.6% of the intracranial aneurysm, ruptured at the mean age of 54 years, and occurred equally in both sexes. The localization of An-ICH was 50% in the middle cerebral artery (MC), 43% in the anterior cerebral artery (AC) and 8% in the internal carotid arterial region. Forty percent of An-ICH were treated conservatively and the outcome was very misery (no useful life and 94% was poor or dead). Sixty percent of An-ICH were treated surgically (neck-clipping and removal of the hematoma) and the outcome was poor (17% useful life and 46% poor and dead). Especially in MC aneurysm, operability was 85% and post-operative useful life was 12% and post-operative poor or dead was 53%. In AC aneurysm, operability was 35% and post-operative useful life was 33% and poor or dead was 33%. These miserable results raised a question of operative indication for An-ICH. The five clinical indices (decerebration, hypertension greater than or equal to 200 mmHg, Hunt-Hess grade V, anisocoria in AC, severe shift of midline structure on X-CT) were selected, because any patients who presented with even only one of them became fatal or vegetative state regardless of conservative or surgical treatment. Assuming them as the non-operative standards, operability decreased in 35% MC, 29% AC, useful life outcome came up to 20% MC, 40% AC, and misery outcome came down to 20% MC, 20% AC.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1985

[Clinical study of spinal intradural arachnoid cyst].

Spinal intradural arachnoid cysts (S-IAC) are rarely found and only 80 cases have been reported since 1915. We report two of them. A 68-year-old male had slowly progressive dull pain in both thighs for about 6 years prior to admission. Neurological examination and plain x-ray on admission showed no abnormality. Myodil myelography showed a S-IAC at T4 vertebral level. Surgical treatment of cyst wall excision resulted in success. The other case, a 39-year-old male suddenly noticed monoparesis and sensory disturbance of left leg in the morning. On admission were observed spastic monoparesis of the left leg, analgesia at right T2-3 cord segment and sensory disturbance (hypesthesia, dysesthesia, paresthesia) below T3-4 cord segment on the left side. Plain X-ray showed no abnormality. Myodil myelography revealed a S-IAC at T3-4 vertebral level. This patient was treated not surgically but conservatively because all neurological deficits but numbness in the left leg almost subsided after bed rest. The patient has experienced no neurological deterioration for five years since then. We also discuss the clinical feature, treatment and etiology of the S-IAC, by analyzing these 82 cases. There is a slight male preponderance. The age ranges between 3 and 66 years (mean 38 years). The duration from onset to admission is 2 days to 30 years. Sixty percent (48/80) of the cases were ill far longer than a year (mean 6.7 years). Of 82 cases, 83% suffered from motor disturbance, 70% had sensery disturbance, 62% local pain and 24% urinary with/without fecal disturbance.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗