Search PubMed⌕ Search

Biomedical subjects

I H Kim

Publications and source records attributed to I H Kim.

At least 73 records · Page 4Linked to original sources
PubMed1999

Involvement of ArcA and Fnr in expression of Escherichia coli thiol peroxidase gene.

To explore the oxygen response regulators involved in thiol peroxidase gene (tpx) expression in Escherichia coli, we constructed a single-copy tpx-lacZ operon fusion and monitored tpx-lacZ expression in various genetic backgrounds. Expression of the tpx-lacZ fusion was increased 4-fold by aerobic growth. Anaerobic expression of tpx-lacZ in either (delta)arcA or delta(fnr) strains was 2.5-fold depressed compared with that of the wild-type strain. The results of immunoblotting experiments also demonstrated that ArcA and Fnr regulatory proteins repressed thiol peroxidase gene expression during anaerobic growth. Inspection of the tpx promoter region revealed putative binding sites for ArcA and Fnr. It thus appears that ArcA and Fnr function as repressors by blocking the binding of RNA polymerase to the tpx promoter in E. coli under anaerobic growth conditions.

Anaerobiosis↗
PubMed1999

Effects of omitting vitamin and trace mineral premixes and(or) reducing inorganic phosphorus additions on growth performance, carcass characteristics, and muscle quality in finishing pigs.

Three experiments were conducted to determine the effects of omitting vitamin and trace mineral premixes and(or) reducing inorganic phosphorus additions to finishing diets on growth performance, carcass characteristics, and muscle quality in pigs. In Exp. 1, a corn-soybean meal-based diet (.70% lysine, .65% Ca, and .55% P) was used as the control. Pigs (n = 128; average initial BW of 85.7 kg) were fed the control diet or the control diet without 1) the vitamin premix, 2) the trace mineral premix, or 3) both premixes. Omitting the premixes had no effect on ADG (P>.39); gain/feed (P>.17); carcass backfat thickness (P>.42); and marbling, color, and firmness of the longissimus muscle (P>.11). In Exp. 2, pigs (n = 128; average initial BW of 86.2 kg) were fed the control diet (.65% Ca and .53% P) used in Exp. 1 and the control diet without 1/3 (.56% Ca and .46% P), 2/3 (.51% Ca and .40% P), or all (.47% Ca and .31% P) of the added monocalcium phosphate (MCP). Omitting up to 2/3 of the MCP increased ADG (quadratic effect, P<.02) and had no effect on meat quality (P>.12), but backfat thickness increased slightly (quadratic effect, P<.02). In Exp. 3, pigs (n = 160; average initial BW of 86.6 kg) were fed the control diet used in Exp. 1 or the control without 1) the vitamin and trace mineral premixes, 2) 2/3 of the MCP, or 3) the premixes and 2/3 of the MCP. Treatment had no effects on ADG (P>.23), gain/feed (P>.94), stomach lesions (P>.37), or serum gamma globulins (P>.08). In conclusion, vitamin and trace mineral premixes and up to 2/3 of the supplemental MCP can be omitted during late finishing (i.e., approximately the final 30 d) to reduce nutrient excesses that increase cost of feeding and nutrients excreted in waste material.

Animal Feed↗
PubMed1999

Survival improvement with combined radio-chemotherapy in the primary central nervous system lymphomas.

The benefits of radio-chemotherapy in HIV-negative primary central nervous system (CNS) lymphomas were analyzed in 40 patients, who received radiotherapy to the brain or craniospinal axis with the total dose of 4460-5940 cGy to the primary tumor. Radiotherapy was followed by systemic chemotherapy, mainly with the cyclophosphamide, doxorubicin, vincristine and prednisolone (CHOP) regimen, in 16 of the patients. Follow-up ranged from four to 95 months with a median of 15 months. The relapse rate was 72.5%, and 83% of the relapses occurred within the radiation field. Median survival was 19 months and the two-year survival rate was 41%. Survival was significantly influenced by treatment method and radiation dose when measured by univariate analysis; median survival and the two-year survival rate was 29 months and 63% after radio-chemotherapy, while 13.5 month and 29% after radiotherapy alone (p= 0.027), and 22 months and 49% with doses of 50 Gy or more, but 12.5 months and 13% with doses less than 50 Gy (p=0.009). However, statistical significance was lost in multivariate analysis. These results might suggest the short-term efficacy of radio-chemotherapy, however, cautious observation is needed to confirm long-term effects.

Adolescent↗
PubMed1999

Gastric malignant stromal tumor with long stalk impacted into duodenum.

Most GIST have traditionally been classified as submucosal origin by upper gastrointestinal series (UGIS) or endoscopy. However, we experienced a gastrointestinal mesenchymal origin tumor expressed by polypoid mass with a long stalk. The gastric outlet was obstructed by the polypoid mass on the duodenum. The microscopic findings of the resected mass showed a stromal cell component by immunohistochemical stain. We experienced gastric stromal tumor found as a polypoid mass with long stalk which was easily detected by endoscopy. It would not have been suspected as a stromal tumor as a result of endoscopic findings only.

Aged↗
PubMed1998

Nucleolus contains signal molecules that constitute membrane-nucleolus linked pathway.

Since there have been very few studies on nucleolar signaling, an attempt was made to establish nucleolar signal pathways which link the cell membrane to the nucleolus for the transfer of extracellular signals. Two pathways were studied. One was the G alpha s mediated cAMP pathway where two signal molecules were yielded, including RII and protein kinase A. The other was the G alpha q mediated DAG/IP3 pathway which yields two signals including protein kinase C and IP3/Ca2+. By the studying isolated nucleoli from resting liver, regenerating liver or weak carcinogen thioacetamide treated liver, it was possible to detect protein kinase A (PKA), protein kinase C (PKC) and RII subunits. In addition, CK2 was detected. It was found that external signals transmitted through G protein coupled receptors could reach into the nucleolus and that physical translocation of signal molecules was an integral step involved in membrane-nucleolus linked pathways. When an in vitro assay of the above signal molecules was carried out using [gamma-32P]-ATP, most kinase dependent phosphorylation was via the major CK2 (more than 95%). Therefore, it is suggested that the major CK2 dependent pathway is involved in 'house keeping' for nucleolar integrity and the minor pathways, dependent on PKA, PKC and others, are involved in subtle regulatory mechanisms such as 'extra-house-keeping' activities by nucleolar chromosomal remodeling.

Animals↗
PubMed1998

Calyculin A modulates activation of the NADPH-oxidase in Me2SO-differentiated HL-60 cells.

Human promyelocytic leukemia cells (HL-60) have been used as a model system in which to study the effects of protein phosphatase inhibitors on NADPH-oxidase activation. Since O2- is generated by NADPH-oxidase, we examined the effect of calyculin A pretreatment on oxidase activation in response to various agonists. When Me2SO-differentiated HL-60 cells were treated with calyculin A prior to the addition of phorbol 12-myristate 13-acetate (PMA), O2- production was inhibited; however, calyculin A enhanced O2- production by N-formyl-methionyl-leucyl-phenylalanine (FMLP). The decreased O2- production seen with calyculin A pretreatment followed by PMA may be due to diminished translocation of the p47-phox and p67-phox, cytosolic components of the oxidase, and inhibition of arachidonic acid release. Interestingly calyculin A pretreatment followed by either agonist significantly enhanced mitogen-activated-protein kinase (MAPK) activity. The differential effects of pretreatment with calyculin A on subsequent oxidase stimulation elicited by FMLP or PMA provide further evidence for substantial heterogeneity in the activation of the respiratory burst.

Arachidonic Acid↗
PubMed1998

Transcriptional control of the glnD gene is not dependent on nitrogen availability in Escherichia coli.

Glutamine synthetase (GS) is one of the most important enzymes in the assimilation of nitrogenous compounds in Escherichia coli and related bacteria. For the control of its activity and biosynthesis, tricyclic cascades of uridylylation/deuridylylation of PII protein, adenylylation/deadenylylation of glutamine synthetase, and phosphorylation/dephosphorylation of Ntr1 are operating, where the regulation of uridylylation/deuridylylation by uridylyl transferase-uridylyl removing enzyme (UT-UR) (the product of the glnD gene) would play the ultimate nitrogen sensing role. However, the possible nitrogen-regulatable element in the upstream of the glnD gene has been debated. In the present experiment, we have cloned and sequenced the four minute regions of the Escherichia coli chromosome, where rpsB, map, glnD, and dapD genes have been identified in sequence. We could localize the transcriptional start site at seven nucleotides upstream of the translation initiation codon by primer extension analysis. The nitrogen dependency of the glnD gene has been analyzed by Northern blot, RNase protection, and promoter-luciferase activity assays. These data suggested a constitutive expression of the glnD gene independent of nitrogen availability. From these results, it could be concluded that the ultimate nitrogen sensing device for the bacteria should be the UT-UR itself, through modulation of its activity in response to the nitrogen status rather than its biosynthetic mechanism.

Amino Acid Sequence↗
PubMed1998

Bacteria in semen used for IVF affect embryo viability but can be removed by stripping cumulus cells by vortexing.

Bacterial contamination of in vitro vs in vivo produced embryos presents a particular danger because of the alteration of the zona pellucida and the use of various biological products during culture. Our objective was to investigate the effects of semen contaminated with bacteria on IVF of bovine oocytes and to determine if removal of cumulus cells by vortexing as opposed to pipetting would reduce contamination and improve subsequent embryonic development. Semen from 5 bulls of the Native Korean breed (Bulls A, B, C, D, E) was used for IVF of matured oocytes. Preliminary studies had shown that the semen from Bulls A, B, D and E but not Bull C was contaminated with various species of common bacteria. After IVF, the cumulus cells surrounding the oocytes were removed either by pipetting or vortexing. Viability and cleavage rates of the resulting zygotes was assessed after 44 h in culture. When cumulus cells were removed by pipetting, only zygotes derived from oocytes that were fertilized with uncontaminated semen from Bull C developed to morula and blastocyst stages; zygotes derived from oocytes that were fertilized with contaminated semen from Bulls A, B, D and E started to degenerate, and the culture media became noticeably turbid. When cumulus cells were removed by vortexing, zygotes derived from oocytes fertilized with either contaminated or uncontaminated semen showed good rates of development (16 to 32%) to morula or blastocyst stages. From these results it can be concluded that the bacteria introduced with the semen contaminated the in vitro system and severely reduced the viability of the embryos. In contrast, complete removal of the cumulus cells with vortexing, as opposed to pipetting, reduced the contamination of the culture medium, allowing embryonic development to take place.

Animals↗
PubMed1998

Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai.

The immunopharmacological characteristics of angelan, a polysaccharide purified from Angelica gigas Nakai, were investigated in relation to the specificity to immune cells. The treatment of angelan increased the expression of IL-2, IL-4, IL-6, and IFN-gamma. The expression of IL-6 and IFN-gamma was rapidly augmented but that of IL-2 responded later. In the case of IL-4, angelan stimulated at early time after exposure but down-regulated thereafter. These results suggested that macrophages and natural killer cells involved in nonspecific immunity were primarily activated and helper T cells were secondarily affected by angelan. Angelan also had lympho-proliferative potential to B cells, specifically. The specificity of angelan was also elucidated in a cell fractionation experiment. The activated B cells by angelan also increased antibody production. The direct activation of B cells, macrophages, and accessory cells and the indirect activation of helper T cells coordinately increased immune functions such as in vitro and in vivo T-dependent immunization and antibody production. The experiment of host resistance to syngeneic tumors also showed that angelan potentiated the immune functions. In conclusion, angelan, a purified polysaccharide from an oriental herbal drug, showed characteristic immunostimulation, which was different from clinically used polysaccharides such as lentinan and PSK.

Adjuvants, Immunologic↗
PubMed1998

Corosolic acid isolated from the fruit of Crataegus pinnatifida var. psilosa is a protein kinase C inhibitor as well as a cytotoxic agent.

Corosolic acid isolated from the fruit of Cratoegus pinnatifida var. psilosa was tested for anticancer activity. Corosolic acid displayed about the same potent cytotoxic activity as ursolic acid against several human cancer cell lines. In addition, the compound displayed antagonistic activity against the phorbol ester-induced morphological modification of K-562 leukemic cells, indicating the suppression of protein kinase C (PKC) activity by the cytotoxic compound. The compound showed PKC inhibition with dose-dependent pattern in an in vitro PKC assay.

Antineoplastic Agents, Phytogenic↗
PubMed1998

The effects of extrusion processing of carbohydrate sources on weanling pig performance.

Three experiments were conducted to study the effects of extrusion processing on growth performance of weanling pigs. In Exp. 1, 350 weanling pigs (initially 4.4 +/- 1.0 kg BW and 10 +/- 2 d of age) were used to study the effects of various carbohydrate sources (corn, cornstarch, broken rice, wheat flour, and grain sorghum), with or without moist extrusion processing, on growth performance in a 5 x 2 factorial arrangement of treatments. No carbohydrate source x extrusion processing interactions were observed (P > .10). Growth performance was not affected by extrusion processing; however, pigs fed corn had poorer growth performance (P < .05) than those fed other carbohydrate sources. In Exp. 2, 360 weanling pigs (initially 5.0 +/- .5 kg BW and 10 +/- 2 d of age) were used to determine the interactive effects of ingredient processing and diet complexity on growth performance. Three processing combinations were used with either a simple or complex diet formulation in a 3 x 2 factorial arrangement of treatments. The three processing conditions were 1) pelleted only (control); 2) corn that was moist-extruded and then the complete diet was pelleted (extruded); or 3) the complete diet was expanded and then pelleted (expanded). Pigs fed extruded diets had a greater improvement in ADG as diet complexity increased than those fed other diets (processing x diet complexity interaction, P < .10). Pigs fed moist-extruded corn had the best growth performance (P < .01). In Exp. 3, 210 weanling pigs (initially 6.8 +/- 1.5 kg BW and 21 +/- 2 d of age) were fed pelleted diets containing nonextruded corn (14.5% gelatinization; control) or corn extruded to provide 38.7, 52.7, 64.4, or 89.3% gelatinization. Average daily gain and ADFI decreased and then increased (P < .05), but apparent digestibility of DM, CP, and energy (P < .01) increased and then decreased with increasing gelatinization. These results indicate that moist extrusion processing of carbohydrate sources has variable effects on growth performance of early-weaned pigs and that the degree of gelatinization does not seem to be a major factor in explaining this variation.

Animal Feed↗
PubMed1998

Influence of dietary lysine on growth performance and carcass characteristics of late-finishing gilts.

We conducted two experiments evaluating the lysine requirement of 91- to 113-kg gilts. Gilts (PIC L326 x C-22) with initial body weights of 42 (Exp. 1) or 39 kg (Exp. 2) were blocked by weight in a randomized complete block design. Experiment 1 included 105 gilts, with seven gilts per pen and five replications (pens) per treatment. Experiment 2 included 125 gilts, with eight gilts per pen and four replications (pens) per treatment. Gilts were fed a corn-soybean meal diet (no crystalline lysine) containing 1.0% total lysine from 39 to 68 kg and .80% total lysine from 68 to 91 kg. In Exp. 1, when the average weight of gilts within a block reached 91 kg, gilts were fed corn-soybean meal diets containing .40, .55, or .70% total lysine. Increasing dietary lysine increased (linear, P < .05) ADG, daily lysine intake (11.3, 15.1, and 19.8 g/d), gain/feed, plasma urea N, and carcass lean percentage but decreased 10th rib fat depth. Because of the linear responses in Exp. 1, Exp. 2 was conducted with corn-soybean meal diets containing .60, .70, .80, or .90% total lysine. Increasing total dietary lysine had no effect on ADG, gain/feed, 10th rib fat depth, or carcass lean percentage (P > .10). Daily lysine intake increased (linear, P < .01) with increasing dietary lysine (18.1, 21.1, 23.9, 26.5 g/d). Based on these results, the total dietary lysine requirement for 91- to 113-kg gilts is approximately .60% total lysine, which corresponds to approximately 18 g/d lysine intake.

Animal Nutritional Physiological Phenomena↗
PubMed1998

Influence of dietary total sulfur amino acids and methionine on growth performance and carcass characteristics of finishing gilts.

We conducted three experiments to determine the sulfur amino acid (SAA) and methionine requirements of finishing gilts. Gilts (PIC Line 326 x C-15, Exp. 1; Line 326 x C-22, Exp. 2 and 3) were blocked by initial weight in randomized complete block designs. In Exp. 1, 64 gilts (initially 54 kg) were fed diets containing either .56 or .44% apparent digestible lysine with increasing SAA levels (63, 70, and 77% of apparent digestible lysine) in a 2 x 3 factorial. A lysine x SAA interaction (P < .10) was observed for ADG and ADFI. Increasing SAA:lysine ratios from 63 to 70% in diets containing .56% apparent digestible lysine increased ADG and ADFI; however, increasing the SAA:lysine ratio in diets containing .44% apparent digestible lysine decreased ADG and ADFI. Pigs fed .56% apparent digestible lysine had higher (P < .05) ADG and gain:feed ratio (G/F) and lower 10th rib fat depth than pigs fed .44% apparent digestible lysine. Increasing the SAA:lysine ratio had no effect on G/F or carcass characteristics. In Exp. 2, 80 gilts (initially 74 kg) were fed diets containing .225, .25, .275, .30, or .325% apparent digestible SAA (45, 50, 55, 60, or 65% of .50% apparent digestible lysine, respectively). Increasing SAA concentrations decreased ADG and G/F (linear, P < .06). In Exp. 3, 105 gilts (initially 72 kg) were fed diets containing .20% apparent digestible cystine and .10, .125, or .15% apparent digestible methionine (20, 25, or 30% of .50% apparent digestible lysine). Increasing digestible methionine increased ADG, ADFI, plasma methionine concentrations (linear, P < .01), and G/F (quadratic, P < .03). The greatest increases in ADG and G/F were observed when apparent digestible methionine was increased from .10 to .125%. Based on these results, the apparent digestible methionine requirement is no greater than 25% of apparent digestible lysine, in diets containing excess cystine. This equates to an apparent digestible SAA:lysine ratio that is no greater than 50%.

Adipose Tissue↗
PubMed1997

In vivo footprinting using N-ethyl,N-nitrosourea: improved resolution of the DNA-protein interactions in the human gamma-globin gene promoter region.

N-Ethyl, N-nitrosourea (ENU) was used as a probing agent in conjunction with a modified ligation-mediated polymerase chain reaction in a new in vivo footprinting procedure. In the present work, we examined the promoter region of the human gamma-globin gene under both uninduced and hemin-induced conditions in K562 cells. In the course of comparing this method with the standard dimethyl sulfate (DMS) in vivo method and previously reported results, we were able to verify our new method. However, discrepancies between these methods were observed at the stage selector element, -50 region, of gamma-globin promoter. Our in vivo footprinting result showed DNA-protein interaction at this region under the hemin-induced condition which was not revealed by the conventional DMS in vivo footprinting method. This approach, using ENU-modified in vivo footprinting, is now being applied to clarify the mechanism of cytotoxic drug-induced fetal hemoglobin augmentation.

DNA↗
PubMed1997

Central neurocytoma: the role of radiation therapy and long term outcome.

BACKGROUND: It has been suggested that the biologic behavior of central neurocytoma is indolent, although little is known regarding the role of radiation therapy and long term outcome. To clarify the role of radiation therapy and long term outcome, the authors retrospectively analyzed 15 cases of central neurocytoma. METHODS: Clinical records and radiologic findings of 15 cases of central neurocytoma diagnosed at Seoul National University Hospital between January 1982 and February 1995 were carefully reviewed. The duration of follow-up was from 18-168 months (mean, 52 months); follow-up images were reviewed and the patient's Karnofsky performance scale (KPS) was assessed up to the time of last follow-up. RESULTS: The age of the patients ranged from 15 to 60 years (mean, 25 years) and the male to female ratio was 11:4. Macroscopic total resection of the tumor was performed in seven patients, two of whom received fractionated radiation therapy. Subtotal removal of the tumor was performed in eight patients, five of whom received this same therapy. In 2 of the 5 patients who did not receive radiation therapy after macroscopic total resection, the tumors recurred 8 and 21 months after surgery, respectively; in contrast, recurrence was not detected in the 2 patients who received fractionated radiation therapy after macroscopic total resection. In all five patients who received fractionated radiation therapy after subtotal resection, the tumor shrank (n = 3) or disappeared (n = 2) during the postsurgical follow-up period ranging from 27-113 months; the tumor began to shrink from 6 months to 2 years after radiation. No change was found in the three patients who did not receive radiation therapy after subtotal resection. At the time of last follow-up, the KPS was greater than 90 in all patients except one who required assistance in his daily activities because of complications from radiation therapy. CONCLUSIONS: It is suggested that even if the biologic behavior of a central neurocytoma is benign, radiation therapy appears to have an effect on tumor control. However, it should be carefully decided whether to use radiation therapy for a residual tumor because radiation can cause delayed complications and the clinical course of subtotally resected patients who do not receive radiation therapy is extremely benign.

Activities of Daily Living↗
PubMed1997

Decursinol angelate: a cytotoxic and protein kinase C activating agent from the root of Angelica gigas.

A cytotoxic compound was purified from the root of Angelica gigas Nakai by normal phase HPLC. As a result of the structure analysis by mass, IR, 1H-NMR, and 13C-NMR spectrometry, the compound was identified as decursinol angelate, a structural isomer of decursin, and characterized originally from Sesei grandivittatum. Decursinol angelate showed in vitro cytotoxicity and protein kinase C activating activities like decursin.

Antineoplastic Agents, Phytogenic↗