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Biomedical subjects

I Gurevich

Publications and source records attributed to I Gurevich.

At least 73 records · Page 4Linked to original sources

Viral hepatitis.

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Cross Infection↗

Excessive levels of gram-negative bacteria in hemodialysis machines because of inadequate cleaning guidelines.

The possibility of sepsis or pyrogenic reaction increases in hemodialysis patients as coliform or other gram-negative counts in the dialysis water reach levels greater than or equal to 1000 colonies/ml. After the introduction of Cobe Century II dialysis machine (Cobe, Lakewood, Colorado), routine testing of dialysis water samples revealed gram-negative bacteria in concentrations up to 45,000 colonies/ml. An epidemiologic investigation was performed to determine the source of the bacterial contamination. The amounts of disinfectant solution were increased, but bacterial counts remained unacceptably high. A study of the fluid path within the machines revealed that part of that path was not reached at all by the disinfectant. In addition, the disinfectant was automatically and improperly diluted. Therefore, increased bacterial counts could not be reduced satisfactorily by using the manufacturer's disinfection guidelines.

Disinfection↗

Presumed autoclave failure due to false-positive spore strip tests.

The sterilizing processes in autoclaves and ethylene oxide sterilizers are challenged on a regular basis with a controlled inoculum of spores from two Bacillus species. Within a two-day period in March 1980, the seven autoclaves at this 550-bed hospital appeared to have failed in their function of killing spores on 18 out of 46 test strips. A shut-down of the autoclaves and a massive investigation failed to identify any mechanical, physical, or human failures. However, after 48 hours, it was found that the broth used as growth medium contained a contaminant, Bacillus coagulans, that resulted in broth turbidity at 55 degree C. Incubating an uninoculated tube of trypticase soy broth (TSB) for quality control at 55 degree C in addition to the usual 37 degree C quality check is a recommended safeguard against such occurrences.

Bacillus↗