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Biomedical subjects

I Greenwald

Publications and source records attributed to I Greenwald.

At least 73 records · Page 4Linked to original sources
PubMed1987

Correlation of the physical and genetic maps in the lin-12 region of Caenorhabditis elegans.

We describe the assembly of a set of overlapping clones from the lin-12 III chromosomal region that spans approximately 600 kb, and the identification of two restriction fragment length polymorphisms, eP6 and eP7, that flank the lin-12 locus. A comparison of the physical map and the genetic map yields approximate measurements of 930 kb/map unit for the eP6--lin-12 interval and 830 kb/map unit for the lin-12--eP7 interval. We interpret these values as supporting the proposal that the apparent clustering of genes observed for C. elegans autosomes results from decreased recombination frequency in clusters and not from nonrandom distribution of genes on the physical map.

Animals↗
PubMed1986

A visible allele of the muscle gene sup-10X of C. elegans.

In this paper, we extend our previous analyses of a set of genes in Caenorhabditis elegans that are involved in muscle structure and function: unc-93 III, sup-9 II, sup-10 X and sup-11 I. We describe an unusual, visible allele of sup-10, examine how this allele interacts genetically with mutations in other genes of this set and propose that the wild-type products of the unc-93 and sup-10 loci may be components of a protein complex. We also describe a new gene of this set, sup-18 III, and the interaction of sup-18 alleles with mutations in the other genes.

Alleles↗
PubMed1985

lin-12, a nematode homeotic gene, is homologous to a set of mammalian proteins that includes epidermal growth factor.

The lin-12 gene of the nematode Caenorhabditis elegans controls certain binary decisions during development. The lin-12 locus was cloned by means of Tc1 transposon tagging: spontaneous lin-12 null alleles were isolated in a genetic background permissive for Tc1 transposition, and seven independently isolated mutations were found to be associated with Tc1 insertion events. All of these Tc1-induced mutations mapped to a single 2.9 kb restriction fragment within a 50 kb region examined. The DNA sequence of this fragment revealed that, although it does not contain the entire gene, it does include three complete exons. These three exons together encode 11 peptide units that are homologous to one another. The repeated peptide motif is also homologous to a set of mammalian proteins that includes epidermal growth factor.

Alleles↗