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Biomedical subjects

I Fujii

Publications and source records attributed to I Fujii.

At least 145 records · Page 8Linked to original sources
PubMed1977

Induction of rectal carcinoma in mice by local x-irradiation.

The pelvic region of random bred female ICR-JCL mice and CF1 mice was irradiated with various doses of X-rays at 1-week intervals to determine the relationship between the X-ray dose and induction of rectal carcinoma. The incidence of rectal carcinoma in ICR mice was zero after a single dose of 2,000 rad of X-rays, but 31% after a single dose of 3,000 rad, 6% after 2 doses of 1,500 rad, 25% after 3 doses of 1,500 rad, 42% after 2 doses of 2,000 rad, and 95% after 3 doses of 2,000 rad. This cancer developed in 70% of CF1 mice exposed to 2 doses of 2,000 rad. No case of this tumor was observed in the control animals not exposed to X-rays. The development of this tumor was found to depend on the X-ray exposure dose. Local X-irradiation of the pelvic region is one of the effective methods for inducing rectal carcinoma in mice. Rectal cancers induced by X-irradiation were adenocarcinoma of the tubular, papillary, and mucinous type, and frequently showed invasive growth into the deep layers of the rectal wall.

Adenocarcinoma↗
PubMed1968

Double-stranded Ribonucleic Acid from Cytoplasmic Polyhedrosis Virus of the Silkworm.

Ribonucleic acid (RNA) was extracted by phenol treatment from cytoplasmic polyhedrosis virus isolated from the midgut of infected silkworms. This RNA appears as threads when precipitated in alcohol. Two components having different sedimentation constants were observed. The molecular weight of the RNA preparation obtained by sedimentation coefficient (weight-averaged) and intrinsic viscosity was about 2 x 10(6) to 3 x 10(6). It was one-half to one-third the size of the calculated molecular weight for an entire RNA molecule in a virion. Electron micrographs of this RNA preparation showed two peaks in the distribution of contour length, at 0.4 and 1.3 mum, which would correspond to molecular weights of 10(6) and 3 x 10(6), respectively. The extracted RNA seemed to split into segments at a preferential breaking point. This RNA was soluble in concentrated salt solution, differing from single stranded high-molecular-weight RNA. The base composition of this RNA was complementary in the ratios of adenosine to uridine and guanosine to cytosine. It contained 43% guanosine plus cytosine. Based on its filamentous appearance by electron microscopy, typical pattern of optical rotatory dispersion and circular dichroism, sharp transition of the optical properties on heating, great hyperchromicity on degradation, nonreactivity with formaldehyde, and resistance to ribonucleases, it is concluded that this RNA is double-stranded and has regular base pairings of guanosine-cytosine and adenosine-uridine.

Journal Article↗