Migration and AIDS.
Explore the source record for details and available documents.
Acquired Immunodeficiency Syndrome↗
Biomedical subjects
I Fernandez
Publications and source records attributed to I Fernandez.
Population study of 3 STR loci in the Basque Country (northern Spain).
The tetrameric STRs, HUMTH01, HUMVWA31A and HUMFES/FPS, were studied in a population from the Basque Country (northern Spain) for their frequency distribution and applicability to identity and paternity testing. All systems conformed to Hardy-Weinberg equilibrium; pairwise comparisons demonstrated the allelic independence between loci, and furthermore, all systems seemed to be in agreement with expectations from the Stepwise Mutation Model (SMM) of the mutation-drift theory, which indicates the homogeneity of the population and suggests a replication slippage mechanism as a possible model for generating alleles. A comparison with other population groups appeared to indicate that frequencies are well conserved in Caucasians, but differ from other racial groups. The calculated parameters "a priori probability of exclusion" (PEX) and "index of discrimination" (ID), show the informativeness of these loci for the determination of identity and relatedness of individuals.
Alleles↗
Infectious complications following pancreatic transplantation: incidence, microbiological and clinical characteristics, and outcome.
The infectious complications following pancreatic transplantation in 34 consecutive recipients were analyzed during a mean follow-up of 39 months. Twenty-seven recipients (79%) developed a mean of 2.1 serious infectious complications. Three of the six deaths (overall mortality, 18%) were infection related. Thirty-three percent of severe infectious episodes were caused by bacteria (72% by gram-positive cocci) and 26% by fungi (87% by Candida species); severe cytomegalovirus (CMV) infection accounted for 33% of infectious complications. CMV disease and organ involvement occurred most frequently in the donor-seropositive/recipient-seronegative group (36%), followed by the donor-seronegative/recipient-seropositive group (25%). In four patients (12%) Epstein-Barr virus (EBV)-related posttransplantation lymphoproliferative disease (PTLD) developed, directly resulting in two deaths. PTLD developed in two of the three EBV-seronegative and two of the 31 EBV-seropositive recipients. Infections due to herpes simplex and zoster viruses and Pneumocystis carinii (2, 3, and 1, respectively) developed in 6 patients. The use of OKT3 for rejection therapy was associated with symptomatic CMV disease and EBV-related PTLD. In summary, severe infectious complications are the main cause of morbidity and death among patients who undergo pancreas transplantation. Aggressive antimicrobial prophylactic regimens are required to decrease the effects of such complications.
Adolescent↗
Median nodule of the upper lip: an autosomal dominant trait.
We describe a total of 18 individuals, in 3 families, with a median nodule of the upper lip. In family 1, the proposita, an 8-month-old infant girl, was otherwise phenotypically normal except for a median nodule of the upper lip. The proposita's elder brother and mother, both phenotypically normal, also had the similar nodule of the upper lip. On the mother's side, the proposita's greatgrandmother, greatgrandaunt, grandfather, greataunt, two aunts, and one female cousin all had a median nodule of the upper lip. In family 2, the proposita, proposita's mother and maternal grandfather had a median nodule of the upper lip. In family 3, the proposita, proposita's father, paternal grandfather, paternal uncle, and cousin had a median nodule of the upper lip. Analysis of 3 families indicates that the condition is an autosomal dominant trait.
Adult↗
Congenital heart defects in Aarskog syndrome.
We report on 10 Japanese individuals from 3 families affected with Aarskog syndrome. Pulmonary stenosis and ventricular septal defect with spontaneous closure were detected respectively, in 2 of them as an uncommon finding. A review documented 169 non-Japanese cases (2 with congenital heart defects), while of 30 Japanese individuals reported till now, 4 (including ours) had cardiac anomalies. We propose that this combination is not coincidental and that in all cases of Aarskog syndrome a cardiac evaluation is indicated.
Abnormalities, Multiple↗
A randomized study of mitoxantrone plus cytarabine versus daunomycin plus cytarabine in the treatment of previously untreated adult patients with acute nonlymphocytic leukemia.
Between May 1985 and November 1988, 143 adult patients with previously untreated acute nonlymphocytic leukemia were randomized to receive mitoxantrone and cytarabine (MTT+Ara-C) or daunomycin and cytarabine (DNM+Ara-C) in order to compare the efficacy and acute and chronic toxicities. Therapy consisted of 3 days of MTT 12 mg/m2/i.v. or DNM 45 mg/m2/i.v.; both groups received Ara-C 100 mg/m2 daily by continuous infusion (CI) for 7 days. Those who failed to achieve a complete remission after one induction course received a second induction course for 2 and 5 days at the same doses. All the patients who achieved complete remission received two consolidations of 2 days of MTT or DNM and 5 days of Ara-C in CI at the same dose as for induction. Of the 72 patients on MTT+Ara-C, 38 (53%) achieved complete remission, compared with 29 (43%) of 67 treated with DNM+Ara-C. Three and 5 patients had partial remission, 7 and 18 failed to respond, 24 and 15 died in the first 21 days of induction, of those treated with MTT+Ara-C or DNM+Ara-C, respectively (p = 0.34). Median duration of complete remission and survival was 185 and 103 days or 165 and 160 days, respectively (p = 0.85). More early deaths were observed with MTT+Ara-C due to greater myelosuppression, and a higher incidence of failure with DNM+Ara-C. No significant differences between treatment groups were observed in 21 categories of adverse events. The results demonstrate similar incidence of complete response, length of duration of complete remission, overall survival, and toxicity with MTT+Ara-C and DNM+Ara-C.
Adult↗
Familial hyperextensible proximal interphalangeal joints.
Hyperextensibility of the proximal interphalangeal joints was noted in 3 males and 6 females in 3 generations of a Japanese family. The proposita, a 14-year-old girl, had hyperextensible proximal interphalangeal joints of the 2nd, 3rd, 4th, and 5th fingers giving "swan-neck" appearance during the extension. She was asymptomatic and had no other features of skin involvement. Her father, younger sister, grandmother, two aunts, and three cousins on the father's side had hyperextensible proximal interphalangeal joints. No instance of male to male transmission was present. The condition was thus inherited as an autosomal or X-linked dominant trait.
Adolescent↗
Avian infectious bronchitis: specific lachrymal IgA level and resistance against challenge.
Since circulating antibody titres against infectious bronchitis virus (IBV) often correlate poorly with protection against re-infection, the lachrymal specific IgA level of IBV vaccinated chickens was evaluated as an indicator of resistance against challenge. For this purpose, the post-vaccination (pv) IBV specific lachrymal IgA response was monitored in 30 chickens at 3-day intervals until a rise of this specific isotype was detected. On day 19 pv, all birds (vaccinated group and non-vaccinated controls) were challenged with the moderately attenuated H-52 IBV strain via the ocular route. Protection was assessed by viral reisolation attempts in SPF embryonated chicken eggs (ECE) from tracheal swabs 4 days after challenge. Virus was detected in all control birds by the second passage in ECE. IBV was reisolated from 27% (eight birds) of all H-120-primed birds after five passages in ECE. These results demonstrate that lachrymal-fluid-specific IgA levels in chickens are associated with resistance against IBV reinfection and are therefore a suitable source of antibodies that could be used for flock profiling.
Animals↗
Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
The presence in the New World of a variant strain of Plasmodium vivax (VK247) containing a unique circumsporozoite (CS) repeat domain was determined by the detection of antibodies to the variant CS protein and by genetic analysis of the CS gene from field isolates. Whole blood specimens were collected on filter paper from patients infected with P. vivax in Mexico and Peru. Plasmodium vivax DNA was eluted from filter paper samples and the CS gene was amplified by the polymerase chain reaction (PCR) and analyzed for the presence of VK247 or VK210 DNA by oligoprobe hybridization. Sera eluted from a companion filter paper sample were screened for antibodies reactive with the predominant and variant repeat peptides by enzyme-linked immunosorbent assays (ELISA) and with sporozoites by the immunofluorescent antibody (IFA) test. All 24 patients were positive by PCR and oligoprobe hybridization for either VK210 (16 of 24), VK247 (3 of 24), or both (5 of 24). Mixed infections were common (5 of 7) in Peru, but were not observed in the Mexican isolates (0 of 17). All three VK247 infections from Mexico occurred in residents of the foothills above Tapachula (P = 0.02). Of patients with smear-positive P. vivax infection, 42% (10 of 24) had detectable antibodies eluted from dried blood dots that were reactive with the CS protein by IFA or ELISA. These findings establish the widespread distribution of the P. vivax variant CS protein in the New World and indicate that dried blood filter paper samples represent a valuable source of material for the serologic and molecular analysis of plasmodial infections.
Animals↗
A delayed-type hypersensitivity-inducing T-cell epitope of Semliki Forest virus mediates effective T-helper activity for antibody production.
The rational development of peptide vaccines requires the identification of both B- and T-cell epitopes. In this study, potential T-helper cell epitopes of Semliki Forest virus (SFV) were identified on the basis of their ability to induce delayed-type hypersensitivity (DTH) in mice using recombinant SFV fragments produced as hybrid proteins with beta-galactosidase in Escherichia coli and synthetic peptides coupled to beta-galactosidase. Although the tested fragments spanned almost the entire amino acid sequence of the structural proteins of SFV, only one DTH-inducing region (located between amino acid 137 and 151 of the SFV E2 membrane protein) was identified. Peptides containing this E2 region stimulated lymph node cells from SFV-primed mice in vitro. The ability of the identified T-cell epitope to induce a specific T-helper response in mice was evaluated using synthetic peptides that contained combinations of the DTH-inducing region and different previously identified linear B-cell epitopes of E2. These peptides proved able to induce an antipeptide IgG response in mice in an H-2d-restricted fashion. One of the peptides was also able to induce high titres of IgG reactive with SFV-infected cells and protected 70-100% of the peptide-immunized mice after challenge with virulent SFV. Our findings suggest that DTH and T-helper activity are mediated by different doses of the same T-cell epitope.
Animals↗
An analysis of panic symptoms during hypercarbia compared to hypocarbia in patients with panic attacks.
Twenty panic disorder patients underwent a 35% CO2 challenge test and a hyperventilation provocation test. CO2-induced anxiety proved to correlate significantly with respiratory symptoms. These symptoms appeared to be considerably more severe during CO2 inhalation than during the hyperventilation provocation test, which induced no significant anxiety.
Administration, Inhalation↗
A method for culturing rat oviduct gamma-aminobutyric acid cells.
A technique for the culture of rat oviduct gamma-aminobutyric acid (GABA) cells is described. The technique involves first explaining the fimbria and preampulla, which are the oviduct divisions with the highest density of GABA cells. The explanted tissue is cultured in a serum-free medium, to propagate the outgrowing cells. Under the experimental conditions we describe, the majority of the cells maintain GABA expression, as determined by immunostaining with a GABA antiserum.
Animals↗
Higher lifetime prevalence of respiratory diseases in panic disorder?
Current and past frequencies of respiratory diseases were assessed in 30 patients with panic disorder, 30 patients with obsessive-compulsive disorder, and 30 patients with eating disorders. Lifetime prevalence of respiratory disorders was significantly higher in patients with panic disorder (47%) than in patients with either obsessive-compulsive disorder (13%) or eating disorder (13%). Point prevalences showed no differences.
Adult↗
[Contribution to the pharmacodynamic study of a ketosulphoxide derivative (omega-methylsulphinyl alpha-acetothiophene). Activity on the CNS].
In order to establish the pharmacological profile of a series of ketosulphoxide derivatives which have been synthesized in the pharmaceutic and organic chemistry department of the Sevilla Pharmacy Faculty, a study of omega-methylsulphinyl alpha-acetothiophene has been initiated. Experiments were carried out on male Swiss mice, using a dosage of 100 and 200 mg/kg. High toxicity tests have been undertaken, followed by an investigation of activity at CNS levels. The results obtained show that the composite mentioned has a marked depressant activity lacking in any significant toxic effects.
Animals↗
Analysis of lymphocyte and oral mucosa cell micronuclei in Cuban paint industry workers.
Twenty-one workers exposed to complex mixtures in the paint industry were compared with a control group of 19 persons from a blood bank in regard to micronuclei frequency in peripheral blood lymphocytes and in oral mucosa cells. The exposed persons showed significantly increased levels of micronuclei frequencies in both types of cells.
Adult↗
Orbital hydatid cyst of Echinococcus oligarthrus in a human in Venezuela.
This study reports the first known case of human hydatid disease caused by the larval stage of Echinococcus oligarthrus. The patient, a native Venezuelan female, presented a single cyst localized intraorbitally behind the left eye (retroocular), which was discovered by computed tomography. The cyst was removed by surgery, and after parasitological studies it was identified as an E. oligarthrus hydatid cyst. This is also the first case of intraorbital hydatid cyst in humans in Venezuela.
Animals↗
Ultrastructure of the oocyst wall formation in Eimeria? beauchampi Leger and Duboscq, 1917, a coccidian parasite of Glossobalanus minutus (Kow.) (Enteropneusta, Hemichordata).
The ultrastructure of the developing oocyst of Eimeria? beauchampi Leger and Duboscq, 1917 was studied from the ventral digestive epithelium of the hepatic region of Glossobalanus minutus (Enteropneusta). A possible building mechanism of the oocyst wall is deduced and discussed from the present data and the available literature. During oocyst wall formation a total of 5 membranes were observed at or near the surface of the parasite among which some wall-forming materials will be stored. The origin and fate of such wall-forming materials are discussed and compared with data from other coccidians. The apparently full-formed wall is made up of an outer, dense layer, a median layer showing a labyrinthic-tubular lattice substructure, and an inner, homogeneous and osmiophilic layer. A micropyle measuring 0.35 micron in diameter is also described.
Animals↗
[Frequently committed errors in reevaluation of patients treated for marginal adult periodontitis].
Explore the source record for details and available documents.
Adult↗