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Biomedical subjects

I Cernak

Publications and source records attributed to I Cernak.

44 records · Page 3Linked to original sources
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[The effect of indobufen on patency in femoro-popliteal/crural bypass using artificial grafts].

The aim of this clinical prospective study was to determine the effect of indobufen upon synthetic graft patency in femoral-popliteal/crural position. 15 operated patients were observed during the three-month period. One day prior to operation patients were given 400 mg of indobufen perorally. The same daily dose was continued on the first postoperative day as well as during the following three months. Blood levels of 6-keto-prostaglandin (PG) F1alpha (stable metabolite of PGI2) and thromboxane (Tx) B2 (stable metabolite of TxA2) were determined by RIA before indobufen administration, i.e., one day and three months postoperatively. The three-month patency of grafts was achieved in 86% of cases. Plasma levels in all observed time periods showed significantly reduced TxB2, increased 6-keto-PGF1alpha, and higher PGI2 levels compared with TxA2 that could suggest the normalization of aggregation/antiaggregation process.

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Brain PGD2 and PGE2 changes during posthaemorrhagic hypovolemia in rats.

This study examined the changes of prostaglandins (PGs) E2 and D2 in brain structures of conscious rats during one hour period following acute bleeding. The analyses were performed in brain structures which are important for cardiovascular homeostasis (medulla oblongata, hypothalamus) and these which are not directly involved in central homeostatic mechanisms but are selectively vulnerable to low-flow states (hippocampus, cortex). Haemorrhage was induced by gradual withdrawal of blood from the cannulated right femoral artery (18 ml/kg, LD30) during a 3-min interval. The brain prostanoid contents were measured by radioimmunoassays. The bleeding induced significant changes in brain prostanoid content which were expressed to the highest extent in medulla oblongata and hypothalamus. The time course of the alterations in PGD2 and PGE2 contents was different in m. oblongata and hypothalamus from that in cortex and hippocampus. It was observed that PGE2 and PGD2 levels rose significantly in brain structures which are important for cardiovascular homeostasis and that their alterations were less expressed with different time course in structures which are selectively vulnerable to ischemia. Therefore, the authors suppose that the prostanoid changes in m. oblongata and hypothalamus are induced by the activation of central homeostatic mechanisms rather than by cerebral ischemia produced by haemorrhagic hypotension.

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PubMedDate not supplied

Do prostaglandins E2, F2 alpha and D2 have a role in the development of idiopathic trigeminal neuralgia?

Idiopathic trigeminal neuralgia (ITN), resistant to the drug treatment was diagnosed in 20 patients using following parameters: clinical feature, usage of Bell-McGill questionnaire, as well as X-ray and neurophysiologic examinations. Peripheral neurectomy was performed in all of them as a palliative therapeutic procedure. In removed nerves of patients (n. infraorbitalis, n. alveolaris inferior), as well as in the same nerves of cadavers, the concentrations of prostaglandins (PGs) E2, F2 alpha and D2 were determined using commercial radioimmunoassay. Significant increase in PGE2 and PGF2 alpha concentration and simultaneous decrease in PGD2 concentration in the nerves of the patients with ITN was noticed. Results demonstrated that the increase in the concentration of hyperalgesic PGE2 and PGF2 alpha as well as the decrease in hypoalgesic PGD2 concentration had an important role in the onset of neuralgic pain in patients with ITN.

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Influence of low level laser irradiation on biochemical processes in brainstem and cortex of intact rabbits.

The influence of low level laser (LLL) irradiation at wavelength at 660 and 904 nm on oxidative stress (lipid peroxidation activity-LP, production of superoxide anion radicals-NBT reduction), activity of enzymes of antioxidative defense (superoxide dismutase-SOD, glutathione reductase-GR) and functional activity of sodium pump (Na+K+ ATPase) in relation with applied wavelength of LLL was investigated. The investigation was performed at the adult rabbits (n = 21) classificated in three groups: control group (C), the group of rabbits irradiated with LLL wavelength 904 nm (CL1) and the group of rabbits irradiated with LLL wavelength 660 nm (CL2). The irradiation was performed in the upper cervical region in the anatomical projection of the brainstem. It was established that LLL induced oxidative stress in the brainstem and the cortex of treated rabbits, independently of applied wavelength of laser beams. The registrated changes in functional activity of sodium pump were dependent on the applied wavelength. The irradiation at 904 nm caused the significant increase of the substrate uptake rate of sodium pump in the brainstem tissue. The irradiation at 660 nm caused the "competitive inhibition" of the sodium pump. Decrease of norepinefrine content in the brainstem of treated rabbits pointed on the indirect mechanism of functional activity of sodium pump as well as the oxidative stress.

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PubMedDate not supplied

[Use of fibrin glue prepared from recycled cryoprecipitate in experimental liver surgery].

Fibrin glue (FG) is a two-component biologic system with adhesive, sealant and topical hemostatic properties, containing fibrinogen (Fg), factor XIII (FXIII), fibronectin (Fn), thrombin, some antifibrinolytic agent if needed and ionized calcium. In this study, FG component 1 was prepared by recycling cryoprecipitation from single-donor plasma. The mean concentrations of Fg, FXIII and Fn were: 54.2 +/- 19.9 g/l, 8.03 +/- 2.3 IU/ml and 3103.1 +/- 148.91 mg/l, respectively. Horizontal tensile strength of FG was 1.076 +/- 0.18 N/cm2 in the average. Using a rat model, the efficacy of the FG-treatment in liver surgery was evaluated on the basis of the 24 hour survival ratio and hematological parameters of the experimental animals and control group. Survival of rats subjected to partial and total lobectomy and FG-treated was significantly (p < 0.001) higher than in FG-non-treated animals. Survival of animals subjected to liver incision was not significantly different, although the differences in hematological parameters were significant (p < 0.001 to p < 0.09) in favor of FG-treated animals. Our findings confirmed that high quality FG can be prepared by recycling cryoprecipitation from single-donor plasma--with sufficient yield of fibrinogen, FXIII and fibronectin and with the risk of disease transmission not greater than with the use of single unit of blood or plasma--which have efficient hemostatic and therapeutic properties.

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