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Biomedical subjects

I Caorsi

Publications and source records attributed to I Caorsi.

At least 37 records · Page 2Linked to original sources
PubMed1984

Immunoreactive kallikrein localization in the rat kidney: an immuno-electron-microscopic study.

The cellular and subcellular localization of immunoreactive kallikrein was studied in the rat kidney using the peroxidase-antiperoxidase (PAP) method for the electron microscope. The effect of various tissue-processing protocols on ultrastructural preservation and immunocytochemical staining was evaluated by fixing kidneys with four different mixtures. The tissues were immunostained and further stained with OsO4 or silver methenamine. The best ultrastructural and immunocytochemical staining was obtained with Zamboni's-glutaraldehyde fixative. The kallikrein-immunoreactive cell type was identified, according to its localization and ultrastructural features, as the connecting-tubule cell. Immunoreactive kallikrein was concentrated mainly in the upper one-third of the cell and at both sides of the nuclei, and to a less extent was associated with the plasma membranes and basolateral infoldings. The immunoreactivity was related to free polyribosomes, the rough endoplasmic reticulum (RER), and the Golgi complex, suggesting that kallikrein is actively synthesized in this particular type of cell.

Animals↗
PubMed1984

Langerhans cells in squamous exocervical carcinoma: a quantitative and ultrastructural study.

The gross morphology, density, distribution, and ultrastructure of the Langerhans cell (LC) in the human exocervix in cases of cervical intraepithelial neoplasia (CIN) and incipient carcinoma were investigated. Two zinc-iodide-osmium (ZIO) procedures were applied to epithelial sheets and to tissue sections for light and electron microscopy. The ZIO methods as well as conventional electron microscopy revealed the presence of LCs in CIN, in the invasive prongs, and in the lymphoid infiltrate of the chorion. The epithelial sheets demonstrated a LC density 3.5 times higher than we have previously reported for the normal exocervix. The region around the external os displayed the highest LC density and the most advanced grades of CIN. The LCs were classified into six types according to the number of processes and secondary branches. The most ramified types were more abundant in the neoplastic exocervix than in the normal exocervix. The LC in all locations of the exocervix with carcinoma showed hypertrophy of the rough and smooth endoplasmic reticula and a large number of filaments. The RER displayed dilated cisternae containing electron-dense material. Specialized contacts between LCs and lymphocytes were an outstanding finding. These findings suggest that the LC might play an important role in the neoplastic process.

Adult↗
PubMed1980

Ultrastructural and morphometric study of the Langerhans cell in the normal human exocervix.

The gross morphology, density, distribution and ultrastructure of the Langerhans cell of the normal human exocervix were investigated. Two standardized zinc-iodide-osmium (ZIO) procedures were applied to epithelial sheets as well as to tissue samples processed for both light and electron microscopy. Conventional electron microscopical techniques were also used. The epithelial sheet preparation allowed the visualization of the whole profile of the Langerhans cell. It was found that the number of cell processes and the degree of their branching varied greatly from one cell to another. The cells were tentatively grouped into five types and it is suggested that they represent different degrees of cell activity. The cells appeared unevenly distributed, but with a preferential location around the external os. In the three cases studied the cell density averaged 8.3 LC/mm2. The ultrastructural study revealed the classical fine structure of the Langerhans cell. The cell processes and their branches contained all the organelles found in the perikaryon, including Golgi complexes and Langerhans cell granules. The two ZIO procedures revealed that the complex inner organization of the granule does not differ from that in the epidermal Langerhans cell. It is concluded that the Langerhans cell is a constant component of the normal human exocervix.

Adult↗