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Biomedical subjects

I Brook

Publications and source records attributed to I Brook.

494 records · Page 28Linked to original sources
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[The primary screening of bifidobacteria and lactobacilli strains to develop effective probiotic preparations based on them].

10 Bifidobacterium strains and 10 Lactobacillus strains were studied for their antagonistic activity with respect to Escherichia coli, Klebsiella ozaenae, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and for their sensitivity to antibiotics, widely used in clinical practice. L. acidophilus strain 5/4, L. acidophilus strain 18/4, B. adolescentis strain UX, B. longum strain 44 exhibited the highest antagonistic activity and the highest degree of antibiotic resistance. The restriction analysis of the chromosomal DNA of these strains was then made and their plasmid content was studied, making it possible to recognize these strains in future in the course of in vivo experiments.

Animals↗
PubMedDate not supplied

[Effect of bifidobacteria and lactobacillus and antibiotics in combination with common gnotobiological isolation on the survival of mice with acute radiation sickness].

The influence of the combined use of bacterial preparations (Bifidobacterium and Lactobacillus) and antibiotics (ciprofloxacin, lomefloxacin and amikacin) on the survival rate of irradiated mice placed under the conditions of general gnotobiological isolation was studied. Bacterial strains used in combination with quinolones (ciprofloxacin, lomefloxacin) significantly increased the mean survival time of the animals (p < 0.05) when introduced in a dose of 1.0 x 10(9) microbial cells per mouse on days 1, 3, 5, 7, 9, 11, 13 and 15 after irradiation. At the same time a short course of treatment with bacterial preparations (two injections on days 5 and 7 after irradiation) proved to be insufficient for increasing the survival rate of the animals. The mean survival time of the irradiated mice was higher after the use of bacterial preparations in combination with lomefloxacin or ciprofloxacin than after their use with amikacin.

Acute Disease↗
PubMedDate not supplied

Long-term follow-up of implant-stabilised overdentures.

The aim of this retrospective study was to assess the long-term outcome of patients provided with implant-stabilised overdentures. Patients who had been entered into a previous trial were identified; the records were available for nineteen patients restored with conventional complete upper dentures and implant-stabilised lower overdentures. The mean follow-up period was 11.5 years (range 5.25-13.5 years). One implant was lost and all patients have successfully worn their prostheses, although most have experienced problems: loosening, loss and fracture of retaining clips, soft tissue complications and fractured bars. Nine patients subsequently had bars replaced with ball attachments. This conversion significantly reduced requirements for maintenance and professional care. Implant-stabilised lower overdentures supported on two implants provide a simple, predictable solution for patients with otherwise poorly retained and unstable lower dentures.

Adult↗
PubMedDate not supplied

Pathogenicity of the Bacteroides fragilis group.

The Bacteroides fragilis group is one of the most important pathogens in polymicrobial infections. The distribution of the different members of the B. fragilis group in clinical infections varies. Bacteroides fragilis accounts for 63 percent of all the group isolates, Bacteroides thetaiotaomicron for 14 percent, Bacteroides vulgatus and Bacteroides ovatus for seven percent each, Bacteroides distasonis for six percent and Bacteroides uniformis for two percent. All members of the group induced bacteremia that was associated with an average mortality of 27 percent. The B. fragilis group resist beta lactam antibiotics by producing the enzyme beta-lactamase. This enzyme can be detected in abscess fluid, and can interfere with the eradication of other bacteria that are susceptible to penicillins and cephalosporins. All members of the B. fragilis group can become encapsulated during an inflammatory process as was demonstrated in a subcutaneous abscess model in mice. Non-encapsulated strains can become encapsulated with the assistance of their aerobic counterparts. These encapsulated strains are more virulent to the host than non-encapsulated strains. This increased virulence can be demonstrated by a higher rate of induction of bacteremia, and a greater enhancement of growth of other bacteria in mixed infection. Antimicrobial therapy directed only at the eradication of the aerobic bacteria did not prevent encapsulation, or reduce the number of Bacteroides species. The virulence of all members of the B. fragilis group highlights the need to direct antimicrobial therapy against all members of this group.

Abscess↗
PubMedDate not supplied

Lactic acid levels in cerebrospinal fluid from patients with systemic lupus erythematosus.

Cerebrospinal fluid (CSF) samples from 22 patients with systemic lupus erythematosus (SLE) and 20 samples from normal controls were studied for lactic acid levels. Ten samples were obtained from SLE patients with active central nervous system (CNS) involvement and 12 from SLE patients with inactive CNS disease. The mean CSF lactic acid level in patients with SLE was 17.24 +/- 1.25 mg/dl (range 31-10.2 mg/dl) which was not statistically different to the normal control mean of 14.75 +/- 0.9 mg/dl (range 9-28 mg/dl). Thus, lactic acid levels in active or inactive CNS disease of SLE appear to be normal. Due to the fact that lactic acid levels are elevated in the CSF of patients with bacterial meningitis, lactic acid levels can be of potential value in the differentiation between bacterial meningitis and CNS involvement of SLE.

Humans↗
PubMedDate not supplied

Frontal sinusitis.

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Bacteria, Anaerobic↗
PubMedDate not supplied

Microbiology of necrotizing fasciitis associated with omphalitis in the newborn infant.

OBJECTIVE: The purpose of this study was to report the aerobic and anaerobic microbiology of periumbilical necrotizing fasciitis in newborn infants. STUDY DESIGN: Retrospective review was done of the author's 20-year experience. RESULTS: Specimens obtained from 11 newborn infants with periumbilical necrotizing fasciitis were cultured for aerobic and anaerobic bacteria. A total of 38 bacterial isolates was recovered: 21 aerobic and facultative and 17 anaerobic. Aerobic or facultative bacteria only were present in 1 specimen (9%), anaerobes only in 2 (18%), and mixed aerobic and anaerobic flora in 8 (73%). Multiple organisms were recovered from all instances and the number of isolates varied from two to six (average 3.5 isolates per specimen). The predominant isolates were Peptostreptococcus sp. (7 isolates); Bacteroides fragilis group (6); streptococcus group B (4); and Staphylococcus aureus, streptococcus group D, Escherichia coli, and Proteus mirabilis (3 each). All patients underwent extensive debridement and resection, and they received supportive and antimicrobial therapy. Six (55%) of the patients died. CONCLUSIONS: These findings illustrate the polymicrobial aerobic-anaerobic flora of periumbilical necrotizing fasciitis.

Anti-Bacterial Agents↗