The effects of juvenile hormone on the prothoracic glands in Galleria mellonella L. II. The JHa-induced changes at the light microscopic level.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
I Blazsek
Publications and source records attributed to I Blazsek.
Electroncytochemical investigation of activity and localization of several enzymes in differentiating myeloid cells.
Explore the source record for details and available documents.
Acid Phosphatase↗
A multifactorial system controlling myeloid cell differentiation and division.
It has been demonstrated that mature granulocytes contain two stimulatory and three inhibitory factors of cell proliferation. Consequently, beyond the negative feedback inhibitor (chalone), myeloid cells are controlled presumably by other negative (inhibitory) and positive (stimulatory) mechanisms, in addition.
Animals↗
Modulative effect of endogenous granuloid inhibitors on cell proliferation.
The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. larger than or equal to 70,000 approximately 11,500 and less than or equal to 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 microng/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 microng/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-2 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibis the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders or magnitude lower concentrations than does 1,2/5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.
Alkylating Agents↗