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Biomedical subjects

I Becker

Publications and source records attributed to I Becker.

82 records · Page 5Linked to original sources
PubMedDate not supplied

Laser-assisted preparation of single cells from stained histological slides for gene analysis.

Individual cells are prepared from histological tissue sections of routinely formalin-fixed and paraffin-embedded tissues using an ultraviolet laser micromanipulator. This technology, in combination with polymerase chain reaction (PCR)-based gene analysis, will enable researchers to routinely detect a variety of nucleic acid abnormalities underlying cancer, infection, and genetic disease with previously unknown sensitivity: at the single cell level. The utility of this technique is demonstrated by PCR amplification and sequencing of the E-cadherin gene, which codes for a homophilic cell-to-cell adhesion molecule, in early gastric carcinomas of the diffuse type of Lauren's classification. The main characteristics of the laser-assisted microdissection technique are high precision without contamination and easy application. The assignment of individual gene sequences to single cells will now provide a direct link between molecular biology on the one hand and histology and pathology on the other.

Cadherins↗
PubMedDate not supplied

[Ependymoma of the thoracic spine. Cervico-brachialgia as the first symptom--a case report].

Most frequently the ependymomas of the central nervous system affect the Conus medullaris and Filum terminale. Ependymomas of the thoracic spine with association of a tumor-caused syringomyelia are extremely rare. The reported 50 years old patient with a thoracic ependymoma realized first symptoms as a shoulder-arm pain caused by the tumor-syrinx. The case report indicates that MRI is the superior imaging modality for primary tumors of the central nervous system. All relevant diagnostic and therapeutic informations derived from the MRI. The article describes the clinical manifestations, the diagnostic way and therapeutic procedure in the problem region thoracic spine.

Diagnosis, Differential↗
PubMedDate not supplied

Pediatric-onset bipolar disorder: a neglected clinical and public health problem.

Bipolar disorder (BPD), probably the most prevalent psychotic disorder in adults, has been relatively neglected or controversial in children and adolescents over the past century. We reviewed the literature on early-onset BPD. Estimates of prevalence, particularly before puberty, are limited by historical biases against pediatric mood disorders and by formidable diagnostic complexity and comorbidity. Although clinical features of pediatric and adult BPD have similarities, pediatric cases probably cannot be defined solely by features characteristic of adult cases. Onset was before age 20 years in at least 25% of reported BPD cases, with some increase in this incidence over the past century. Pediatric BPD is familial more often than is adult-onset BPD, may be associated with a premorbid cyclothymic or hyperthymic temperament, and can be precipitated by antidepressant treatment. Pediatric BPD episodes frequently include irritability, dysphoria, or psychotic symptoms; they are commonly chronic and carry high risks of substance abuse and suicide. BPD is often recognized in adolescents, but the syndrome or its antecedents are almost certainly underrecognized and undertreated in children. Controlled studies of short- and long-term treatment, course, and outcome in this disorder remain strikingly limited, and the syndrome urgently requires increased clinical and scientific interest.

Adolescent↗
PubMedDate not supplied

Invasive amebiasis in a spider monkey (Ateles geoffroyi). Case report and a short review of the literature of amebiasis in non-human primates.

A spider monkey (Ateles geoffroyi) studied at the San Juan de Aragon Zoo died with symptoms of amoebic dysentery verified by coprologic investigation of the parasite, his female partner was cured with conventional antiamoebic treatment. At autopsy widespread ulcerations in the colon and multiple liver abscesses were found, containing trophozoites of Entamoeba histolytica. Both lesions were identical to those observed in human amoebiasis. A short review of the literature in nonhuman primates infections points out that the so-called spontaneous amoebiasis seen in colonies of captive monkeys, and transmission to their newborns within the same species of monkeys, have been in contact with humans. It is proposed that amoebiasis in monkeys should be considered as an excellent model for experimental studies on amoebiasis because other phylogenetically distant species have shown different pathology or are resistant to the infection. Also, it should raise concern that monkeys may become carriers of cysts and trophozoites of virulent strains of E. histolytica. Entamoeba histolytica DNA hybridization techniques should be considered for comparing genomic similarities with other protozoa, including the genera Amoeba to establish its pattern of evolution.

Animals↗