Search PubMed⌕ Search

Biomedical subjects

I Beck

Publications and source records attributed to I Beck.

43 records · Page 3Linked to original sources
PubMed1980

Changes in maternal peripheral leucocytes around delivery.

Changes in leucocyte populations and lymphocyte transformation with PHA were measured serially in 12 pregnant women, before and after delivery. There was a significant increase in the total numbers of leucocytes and neutrophils at delivery and for the 24 hours post partum. Lymphocyte counts increased, but not at significant levels, at or following delivery. Lymphocyte transformation rates were significantly higher before delivery and at 24 hours post partum, than at delivery or 2 hours post partum. Although plasma suppressor factors were found before and after delivery, plasma taken at delivery or 2 hours post partum was significantly more suppressive for nonpregnant lymphocytes. It is recommended that maternal blood samples should be taken before delivery for assays of maternal immunocompetence or maternofetal mixed leucocyte reactions.

Blood Specimen Collection↗
PubMedDate not supplied

Detection and quantification of multiple drug resistance mutations in HIV-1 reverse transcriptase by an oligonucleotide ligation assay.

OBJECTIVES: To develop an assay for the early detection and quantification of minor human immunodeficiency virus-1 populations bearing multiple drug resistance (MDR) mutations. STUDY DESIGN/METHODS: The oligonucleotide ligation assay (OLA) is based on ligation of probe and detector oligonucleotides annealed to a polymerase chain reaction amplicon strand with detection by an enzyme immunoassay. In OLA-MDR, oligonucleotides were designed to detect MDR mutations. The method was validated with wild-type and MDR mutant clones mixed at different proportions. RESULTS: K103N mutants were detected as minor populations (5%-30%) by OLA in 6 of 18 samples from patients treated with nonnucleoside reverse transcription inhibitors and classified as wild type by sequencing. In one patient, the kinetics of the increase of MDR mutants could be followed in sequential samples, with K103N being detected earlier by OLA than by sequencing. Q151M mutants were detected as minor populations (13%-24%) by OLA but not by sequencing in 4 samples. CONCLUSIONS: Oligonucleotide ligation assay MDR exhibits higher sensitivity than sequencing for detection of minor MDR mutant populations.

Drug Resistance, Multiple, Viral↗